Alignments
Protein Domains
ASAP ID
Names
hycE
hevE
JW2691
ECK2716
b2721
Product
formate hydrogenlyase subunit
Type
CDS
Location
#
Contig
Strand
Left
Right
Length (b.p.)
Length (a.a.)
1
Chromosome
complement
2844762
2846471
1710
570
Sequence
Context
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Protein Families
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Family
Annotated
IPR001268 (IPR001268 NADH:ubiquinone oxidoreductase, 30kDa subunit)
Apr 2008
IPR014364 (IPR014364 (DEPRECATED) [NiFe]-hydrogenase-3-type complex, fused large subunit/NADH:quinone oxidoreductase, subunit NuoCD)
Apr 2008
IPR014656 (IPR014656 (DEPRECATED) [NiFe]-hydrogenase-3-type complex, fused large subunit)
Apr 2008
IPR014029 (IPR014029 NADH:ubiquinone oxidoreductase, 49kDa subunit, conserved site)
Apr 2008
IPR001501 (IPR001501 Nickel-dependent hydrogenase, large subunit)
Apr 2008
IPR001135 (IPR001135 NADH-quinone oxidoreductase, subunit D)
Apr 2008
IPR037232 (IPR037232 NADH:ubiquinone oxidoreductase, 30kDa subunit superfamily)
Jul 2018
IPR029014 (IPR029014 [NiFe]-hydrogenase, large subunit)
Jul 2018
PF00329 (PF00329 Respiratory-chain NADH dehydrogenase, 30 Kd subunit)
Jul 2018
PF00374 (PF00374 Nickel-dependent hydrogenase)
Jul 2018
PF00346 (PF00346 Respiratory-chain NADH dehydrogenase, 49 Kd subunit)
Jul 2018
Gene Ontology
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Annotations
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Type
Annotation
Evidence
Annotated
name
hycE
Published Annotation -
GenBank Accession Number: U00096
Reference: Escherichia coli MG1655
Oct 1998
synonym
JW2691
Published Annotation -
PubMed ID: 16397293
Jan 2006
synonym
ECK2716
Published Annotation -
PubMed ID: 16397293
Jan 2006
synonym
hevE
Published Annotation -
GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
locus tag
b2721
Unique Identifier -
Origin: Guy Plunkett III, guy@genome.wisc.edu
May 2004
product
formate hydrogenlyase subunit
Published Annotation -
EcoCyc Accession Number: EG10478
Reference: Escherichia coli K-12 MG1655
Oct 2021
alternate product name
hydrogenase 3, large subunit
Published Annotation -
PubMed ID: 16397293
Jan 2006
alternate product name
hydrogenase 3, large subunit (part of FHL complex)
Published Annotation -
Database Name: GenProtEC
Jan 2006
alternate product name
large subunit of hydrogenase 3 (part of FHL complex)
Published Annotation -
GenBank Accession Number: U00096
Reference: Escherichia coli MG1655
Jan 2006
protein family
IPR014364 (DEPRECATED) [NiFe]-hydrogenase-3-type complex, fused large subunit/NADH:quinone oxidoreductase, subunit NuoCD
Protein Sequence Similarity -
InterPro Domain: IPR014364
Reference: Escherichia coli K-12 MG1655
Apr 2008
protein family
IPR001268 NADH:ubiquinone oxidoreductase, 30kDa subunit
Protein Sequence Similarity -
InterPro Domain: IPR001268
Reference: Escherichia coli K-12 MG1655
Apr 2008
protein family
PF00329 Respiratory-chain NADH dehydrogenase, 30 Kd subunit
Protein Sequence Similarity -
PFAM Model Name: PF00329
Reference: Escherichia coli K-12 MG1655
Jul 2018
protein family
PF00374 Nickel-dependent hydrogenase
Protein Sequence Similarity -
PFAM Model Name: PF00374
Reference: Escherichia coli K-12 MG1655
Jul 2018
protein family
PF00346 Respiratory-chain NADH dehydrogenase, 49 Kd subunit
Protein Sequence Similarity -
PFAM Model Name: PF00346
Reference: Escherichia coli K-12 MG1655
Jul 2018
protein family
IPR029014 [NiFe]-hydrogenase, large subunit
Protein Sequence Similarity -
InterPro Domain: IPR029014
Reference: Escherichia coli K-12 MG1655
Jul 2018
protein family
IPR037232 NADH:ubiquinone oxidoreductase, 30kDa subunit superfamily
Protein Sequence Similarity -
InterPro Domain: IPR037232
Reference: Escherichia coli K-12 MG1655
Jul 2018
protein family
IPR014656 (DEPRECATED) [NiFe]-hydrogenase-3-type complex, fused large subunit
Protein Sequence Similarity -
InterPro Domain: IPR014656
Reference: Escherichia coli K-12 MG1655
Apr 2008
protein family
IPR001501 Nickel-dependent hydrogenase, large subunit
Protein Sequence Similarity -
InterPro Domain: IPR001501
Reference: Escherichia coli K-12 MG1655
Apr 2008
protein family
IPR014029 NADH:ubiquinone oxidoreductase, 49kDa subunit, conserved site
Protein Sequence Similarity -
InterPro Domain: IPR014029
Reference: Escherichia coli K-12 MG1655
Apr 2008
protein family
IPR001135 NADH-quinone oxidoreductase, subunit D
Protein Sequence Similarity -
InterPro Domain: IPR001135
Reference: Escherichia coli K-12 MG1655
Apr 2008
EC number
1.12.7.2
Published Annotation -
GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
EC number
7.1.1.2
Published Annotation -
Database Name: EC2GO: GO:0008137 Reference: Escherichia coli K-12 MG1655
Dec 2019
EC number
1.12.-.-
Protein Sequence Similarity -
InterPro Domain: IPR001501
Reference: Escherichia coli K-12 MG1655
Dec 2008
EC number
1.6.-.-
Protein Sequence Similarity -
InterPro Domain: IPR014029
Reference: Escherichia coli K-12 MG1655
Dec 2008
MultiFun
6.1
(cell structure; membrane )
Published Annotation -
MultiFun Database Name: MultiFun
Feb 2002
MultiFun
7.3
(location of gene products; inner membrane )
Published Annotation -
MultiFun Database Name: MultiFun
Feb 2002
MultiFun
1.3.5
(metabolism; energy metabolism, carbon; fermentation )
Published Annotation -
MultiFun Database Name: MultiFun
Feb 2002
MultiFun
1.3.7
(metabolism; energy metabolism, carbon; anaerobic respiration )
Published Annotation -
MultiFun Database Name: MultiFun
Feb 2002
codon start
1
Published Annotation -
GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
db xref
UniProtKB:P16431
Published Annotation -
Swiss-Prot Accession Number: P16431
Jan 2005
db xref
GI:1789076
Published Annotation -
GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
db xref
UniProtKB/Swiss-Prot:P16431
Published Annotation -
GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
db xref
ASAP:ABE-0008944
Published Annotation -
GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
db xref
EcoGene:EG10478
Published Annotation -
GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
db xref
GeneID:947396
Published Annotation -
Entrez Gene Database ID: 947396
Reference: Escherichia coli K-12 MG1655
Jun 2009
function
enzyme; Energy metabolism, carbon: Fermentation
Published Annotation -
GenBank Accession Number: U00096
Reference: Escherichia coli MG1655
Oct 1998
note
large subunit of hydrogenase 3 (part of FHL complex); GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0006113 - fermentation; GO_process: GO:0009061 - anaerobic respiration
Published Annotation -
GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
protein id
AAC75763.1
Published Annotation -
GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
subcellular localization
Cytoplasmic
Predicted by PSORTb -
PSORTb Final_Score: 5.41 Reference: Escherichia coli K-12 MG1655
Feb 2008
subcellular localization
Periplasmic
Predicted by PSORTb -
PSORTb Final_Score: 4.48 Reference: Escherichia coli K-12 MG1655
Feb 2008
subsystem
component of in silico model iMC1010
Experimental -
PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655
Mar 2009
subsystem
component of in silico model iJR904
Experimental -
PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655
Feb 2009
subsystem
component of in silico model iAF1260
Experimental -
PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655
Jan 2009
transl table
11
Published Annotation -
GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
GO biological process
GO:0006113 fermentation
Ontology Mapping -
Source and Dest Ontology: MultiFun to GO
Mar 2004
GO biological process
GO:0006118 (OBSOLETE) electron transport
GO Inferred from Electronic Annotation -
Database Name:Identifier: INTERPRO:IPR014364 Reference: Escherichia coli K-12 MG1655
Apr 2008
GO biological process
GO:0055114 (OBSOLETE) oxidation-reduction process
GO Inferred from Electronic Annotation -
Database Name:Identifier: InterPro2GO: IPR001268, IPR001135 Reference: Escherichia coli K-12 MG1655
Jul 2018
GO biological process
GO:0009061 anaerobic respiration
Ontology Mapping -
Source and Dest Ontology: MultiFun to GO
Mar 2004
GO cellular component
GO:0005737 cytoplasm
GO Inferred from Electronic Annotation -
PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655
Feb 2008
GO cellular component
GO:0030288 outer membrane-bounded periplasmic space
GO Inferred from Electronic Annotation -
PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655
Feb 2008
GO cellular component
GO:0019866 organelle inner membrane
Ontology Mapping -
Source and Dest Ontology: MultiFun to GO
Mar 2004
GO molecular function
GO:0016491 oxidoreductase activity
GO Inferred from Electronic Annotation -
Database Name:Identifier: INTERPRO:IPR014656 Reference: Escherichia coli K-12 MG1655
Apr 2008
GO molecular function
GO:0008901 ferredoxin hydrogenase activity
GO Inferred from Electronic Annotation -
Database Name:Identifier: INTERPRO:IPR001501 Reference: Escherichia coli K-12 MG1655
Apr 2008
GO molecular function
GO:0008137 NADH dehydrogenase (ubiquinone) activity
GO Inferred from Electronic Annotation -
Database Name:Identifier: InterPro2GO: IPR001268 Reference: Escherichia coli K-12 MG1655
Jul 2018
GO molecular function
GO:0048038 quinone binding
GO Inferred from Electronic Annotation -
Database Name:Identifier: InterPro2GO: IPR001135 Reference: Escherichia coli K-12 MG1655
Jul 2018
GO molecular function
GO:0051287 NAD binding
GO Inferred from Electronic Annotation -
Database Name:Identifier: INTERPRO:IPR001135 Reference: Escherichia coli K-12 MG1655
Apr 2008
GO molecular function
GO:0016651 oxidoreductase activity, acting on NAD(P)H
GO Inferred from Electronic Annotation -
Database Name:Identifier: INTERPRO:IPR001135 Reference: Escherichia coli K-12 MG1655
Apr 2008
GO molecular function
GO:0016151 nickel cation binding
GO Inferred from Electronic Annotation -
Database Name:Identifier: INTERPRO:IPR001501 Reference: Escherichia coli K-12 MG1655
Apr 2008
GO molecular function
GO:0009055 electron transfer activity
GO Inferred from Electronic Annotation -
Database Name:Identifier: INTERPRO:IPR001135 Reference: Escherichia coli K-12 MG1655
Apr 2008
GO molecular function
GO:0051539 4 iron, 4 sulfur cluster binding
GO Inferred from Electronic Annotation -
Database Name:Identifier: INTERPRO:IPR014656 Reference: Escherichia coli K-12 MG1655
Apr 2008
Comparative
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Experimental
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Strains
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Strains with mutations in this feature.
Strain Name Strain Type Strain Information Strain Request FeatureID Name Mutation Details Experiments Using Strain Submitter FB20903 mutant Tn5 Insertion Request the Strain ABE-0008944 hycE hycE::Tn5KAN-I-SceI at position 790 in Minus orientation ,- pKD46 5 Blattner Laboratory
FB20904 mutant Tn5 Insertion Request the Strain ABE-0008944 hycE hycE::Tn5KAN-I-SceI at position 790 in Minus orientation ,+ pKD46 0 Blattner Laboratory
Context Table
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Listed below are features that lie nearby this feature.
Features encoded in multiple intervals may
appear more than once in the table. Features that span the origin
will have a left endpoint greater than the right endpoint.
Features overlapping the region 2839762 to 2851471
FeatureID Name Feature Type Strand Left End Right End Relationship ABE-0000001 source forward 1 4641652 Contains
ABE-0008926 ascF CDS forward 2839524 2840981 Downstream
ABE-0008928 ascB CDS forward 2840990 2842414 Downstream
ABE-0285541 repeat_region forward 2842423 2842501 Downstream
ABE-0008931 hycI CDS complement 2842573 2843043 Downstream
ABE-0008933 hycH CDS complement 2843036 2843446 Downstream
ABE-0008936 hycG CDS complement 2843443 2844210 Downstream
ABE-0008942 hycF CDS complement 2844210 2844752 Downstream
ABE-0008944 hycE CDS complement 2844762 2846471 Matches
ABE-0008946 hycD CDS complement 2846489 2847412 Upstream
ABE-0008948 hycC CDS complement 2847415 2849241 Upstream
ABE-0008950 hycB CDS complement 2849238 2849849 Upstream
ABE-0008952 hycA CDS complement 2849974 2850435 Upstream
ABE-0008958 hypA CDS forward 2850647 2850997 Upstream
ABE-0008960 hypB CDS forward 2851001 2851873 Upstream
Links download
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Release Notes
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All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.