Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0008950 (hycB) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0008950 showshow history
Names
hycB hevB JW2694 ECK2719 b2724
Product
formate hydrogenlyase subunit
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 2849238 2849849 612 204
Sequence

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View sequence with flanking regions: bases upstream and bases downstream

Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR001450 (IPR001450 (DEPRECATED) 4Fe-4S binding domain) Apr 2008
Automated Process Approved IPR017896 (IPR017896 4Fe-4S ferredoxin-type, iron-sulphur binding domain) Jul 2018
Automated Process Approved PF13247 (PF13247 4Fe-4S dicluster domain) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Uncurated GO biological process GO:0006113 fermentation Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO biological process GO:0009061 anaerobic respiration Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0051536 iron-sulfur cluster binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0009055 electron transfer activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name hycB Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym JW2694 Published Annotation - PubMed ID: 16397293 Jan 2006
Automated Process Approved synonym hevB Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved synonym ECK2719 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b2724 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product formate hydrogenlyase subunit Published Annotation - EcoCyc Accession Number: EG10475
Reference: Escherichia coli K-12 MG1655 		Oct 2021
Person Uncurated alternate product name hydrogenase-3, Fe-S subunit (part of FHL complex) Published Annotation - Database Name: GenProtEC Jan 2006
Person Approved alternate product name probable small subunit of hydrogenase-3, iron-sulfur protein (part of formate hydrogenlyase (FHL) complex) Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Person Approved alternate product name hydrogenase 3, Fe-S subunit Published Annotation - PubMed ID: 16397293 Jan 2006
Protein Family
Automated Process Approved protein family PF13247 4Fe-4S dicluster domain Protein Sequence Similarity - PFAM Model Name: PF13247
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR001450 (DEPRECATED) 4Fe-4S binding domain Protein Sequence Similarity - InterPro Domain: IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR017896 4Fe-4S ferredoxin-type, iron-sulphur binding domain Protein Sequence Similarity - InterPro Domain: IPR017896
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Automated Process Approved EC number 1.12.7.2 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Uncurated MultiFun 1.3.5 (metabolism; energy metabolism, carbon; fermentation) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 1.3.7 (metabolism; energy metabolism, carbon; anaerobic respiration) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG10475 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P0AAK1 Published Annotation - Swiss-Prot Accession Number: P0AAK1 Jan 2006
Automated Process Approved db xref GeneID:948002 Published Annotation - Entrez Gene Database ID: 948002
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref UniProtKB/Swiss-Prot:P0AAK1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:1789079 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0008950 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved function putative enzyme; Energy metabolism, carbon: Fermentation Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note probable small subunit of hydrogenase-3, iron-sulfur protein (part of formate hydrogenlyase (FHL) complex); GO_process: GO:0006113 - fermentation; GO_process: GO:0009061 - anaerobic respiration Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC75766.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Predicted by PSORTb - PSORTb Final_Score: 9.97
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Person Uncurated GO biological process GO:0009061 anaerobic respiration Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Person Uncurated GO biological process GO:0006113 fermentation Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0051536 iron-sulfur cluster binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0009055 electron transfer activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0002654 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002830 hycB CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0001718 hyfA CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0004091 hyfA CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0002732 hycB CDS
Ortholog
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0004191 hyfA CDS
Automated Process Uncurated Go view Cedecea davisae ATCC 33431 [T] ALZ-0004204 CDS
Automated Process Approved Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0003548 hycB CDS
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0004276 hycB CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0004940 CDS
Person Uncurated Go view Enterobacter sp. 638 AEL-0003897 hycB CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0002828 hycB CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0008263 hycB CDS
Person Approved Go view Escherichia coli 536 (UPEC) AEF-0002716 hycB CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0008439 CDS
Person Approved Go view Escherichia coli APEC O1 (APEC) AEG-0002723 hycB CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0001569 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0006496 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0007427 hycB CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0007012 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0079440 hycB CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0003111 CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0003619 CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0008156 hycB CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0008828 hycB CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0003182 hycB CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0007449 hycB CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0001291 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0007314 hycB CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0005835 hycB CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0005310 hycB CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0008063 hycB CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0008811 hycB CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0007387 hycB CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0006605 hycB CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0007429 hycB CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0008247 hycB CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0008462 hycB CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0007911 hycB CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0008282 hycB CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0027702 hycB CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0006369 hyfA_1 CDS
Automated Process Approved Go view Escherichia coli HS ADR-0007535 hycB CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0003196 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0002553 hycB CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0002793 hycB CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0003569 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0003207 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0003136 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0003040 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0177288 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0003029 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0002847 hycB CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0133616 hycB CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0000872 CDS
Person Approved Go view Escherichia coli UTI89 (UPEC) AEA-0003096 hycB CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0003070 hycB CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0001394 CDS
Automated Process Uncurated Go view Pantoea sp. At-9b AGM-0007654 CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0007140 hycB CDS
Automated Process Uncurated Go view Salmonella Arizonae CDC 346-86 BCD-0000123 CDS
Person Approved Go view Salmonella Choleraesuis SC-B67 ADB-0003262 hycB CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0007418 hycB CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0008815 hycB CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0008360 hycB CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0006550 hycB CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0007399 hycB CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0006357 hycB CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0005397 hycB CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0005414 hycB CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0006522 hycB CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0007414 hycB CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0005645 hycB CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0002738 CDS
Person Approved Go view Salmonella Paratyphi A ATCC 9150 ADA-0002709 hycB CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0003561 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0007578 hycB CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0005646 hycB CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0007076 hycB CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0005285 hycB CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0111414 hycB CDS
Person Approved Go view Salmonella Typhi Ty2 ABS-0084471 hycB CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0003451 hycB CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0098929 hycB CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0006446 hycB CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0006791 hycB CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0008230 hycB CDS
Person Uncurated Go view Serratia proteamaculans 568 AEN-0008967 hycB CDS
Automated Process Uncurated Go view Shigella boydii BS512 ADJ-0008564 hycB CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0003340 hycB CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0074099 hycB CDS
Person Approved Go view Shigella flexneri 301 ABT-0088994 hycB CDS
Person Approved Go view Shigella flexneri 8401 BAG-0002970 hycB CDS
Person Approved Go view Shigella sonnei 046 ADD-0003140 hycB CDS
Automated Process Uncurated Go view Yersinia enterocolitica 8081 BAH-0008051 hyfA CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB20907mutantTn5 InsertionRequest the StrainABE-0008950hycBhycB::Tn5KAN-I-SceI at position 412 in Plus orientation ,+ pKD460Blattner Laboratory
FB20908mutantTn5 InsertionRequest the StrainABE-0008950hycBhycB::Tn5KAN-I-SceI at position 412 in Plus orientation ,- pKD465Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 2844238 to 2854849
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0008942hycFCDScomplement28442102844752Downstream
ABE-0008944hycECDScomplement28447622846471Downstream
ABE-0008946hycDCDScomplement28464892847412Downstream
ABE-0008948hycCCDScomplement28474152849241Overlaps Downstream
ABE-0008950hycBCDScomplement28492382849849Matches
ABE-0008952hycACDScomplement28499742850435Upstream
ABE-0008958hypACDSforward28506472850997Upstream
ABE-0008960hypBCDSforward28510012851873Upstream
ABE-0008962hypCCDSforward28518642852136Upstream
ABE-0008965hypDCDSforward28521362853257Upstream
ABE-0008967hypECDSforward28532542854264Upstream
ABE-0008971fhlACDSforward28543382856416Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.