Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0009478 (uacT) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0009478 showshow history
Names
uacT JW5470 ECK2883 ygfU b2888
Product
urate:H+ symporter
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 3031367 3032815 1449 483
Sequence

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View sequence with flanking regions: bases upstream and bases downstream

Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR006042 (IPR006042 Xanthine/uracil permease) Apr 2008
Automated Process Approved IPR006043 (IPR006043 Xanthine/uracil/vitamin C permease) Apr 2008
Automated Process Approved IPR017588 (IPR017588 Uric acid permease UacT-like) Jul 2018
Automated Process Approved PF00860 (PF00860 Permease family) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Approved GO biological process GO:0006810 transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006042
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO biological process GO:0055085 transmembrane transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR006043, IPR017588, IPR006042
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006042
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0016021 integral component of membrane GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR017588
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO molecular function GO:0022857 transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR006043, IPR017588
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0005215 transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006042
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Automated Process Approved name uacT Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved synonym JW5470 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym ECK2883 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym ygfU Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved locus tag b2888 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product urate:H+ symporter Published Annotation - EcoCyc Accession Number: G7507
Reference: Escherichia coli K-12 MG1655 		Jan 2021
Person Approved alternate product name putative permease Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Person Uncurated alternate product name putative transport protein (NCS2 family) Published Annotation - Database Name: GenProtEC Jan 2006
Automated Process Approved alternate product name uric acid permease Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved alternate product name predicted transporter Published Annotation - PubMed ID: 16397293 Jan 2006
Protein Family
Automated Process Approved protein family IPR017588 Uric acid permease UacT-like Protein Sequence Similarity - InterPro Domain: IPR017588
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR006043 Xanthine/uracil/vitamin C permease Protein Sequence Similarity - InterPro Domain: IPR006043
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR006042 Xanthine/uracil permease Protein Sequence Similarity - InterPro Domain: IPR006042
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF00860 Permease family Protein Sequence Similarity - PFAM Model Name: PF00860
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Person Uncurated MultiFun 6.1 (cell structure; membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 7.3 (location of gene products; inner membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 4.2.A.40 (transport; Electrochemical potential driven transporters; Porters (Uni-, Sym- and Antiporters); The Nucleobase:Cation Symporter-2 (NCS2) Family) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0009478 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:Q46821 Published Annotation - Swiss-Prot Accession Number: Q46821 Jan 2006
Automated Process Approved db xref GI:87082181 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:949017 Published Annotation - Entrez Gene Database ID: 949017
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref EcoGene:EG13071 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved function transport; Transport of small molecules: Nucleosides, purines, pyrimidines Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved note putative permease; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC75926.2 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Membrane Predicted by PSORTb - PSORTb Final_Score: 10.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Uncurated GO biological process GO:0055085 transmembrane transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR006043, IPR017588, IPR006042
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO biological process GO:0006810 transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006042
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006042
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0016021 integral component of membrane GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR017588
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO molecular function GO:0022857 transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR006043, IPR017588
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0005215 transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006042
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0005639 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0003534 ygfU CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0003547 ygfU CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0004276 uacT CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0003303 uacT CDS
Ortholog
Automated Process Approved Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0003767 ygfU CDS
Automated Process Uncurated Go view Erwinia amylovora ATCC 49946 ACW-0298852 CDS
Automated Process Uncurated Go view Erwinia tasmaniensis Et1/99 [T] AFI-0002697 CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0005959 CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0002911 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0008257 CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0002888 ygfU CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0001391 uacT CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0006302 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0005764 CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0006157 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0079625 ygfU CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0003312 ygfU CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0003822 pbuX CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0009172 CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0007182 CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0003343 ygfU CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0007649 CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0004258 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0007514 CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0006034 CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0005511 CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0005806 CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0009013 CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0008376 CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0006815 CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0005799 CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0006310 CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0005621 CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0007732 CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0006050 CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0027898 ygfU CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0008395 uacT CDS
Automated Process Approved Go view Escherichia coli HS ADR-0007726 CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0003368 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0002704 ygfU CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0002959 ygfU CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0003387 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0003528 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0003255 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0003214 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0003212 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0003018 CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0133802 ygfU CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0001055 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0003281 ygfU CDS
Person Uncurated Go view Hafnia alvei ATCC 13337 [T] AMD-0002292 pbuX CDS
Person Approved Go view Shigella boydii 227 ADV-0003398 ygfU CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0074249 ygfU CDS
Person Approved Go view Shigella flexneri 301 ABT-0089127 ygfU CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0003133 ygfU CDS
Person Approved Go view Shigella sonnei 046 ADD-0003324 ygfU CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0001532 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0000330 uacT CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB22497mutantTn5 InsertionRequest the StrainABE-0009478uacTygfU::Tn5KAN-I-SceI at position 536 in Plus orientation ,+ pKD460Blattner Laboratory
FB23238mutantTn5 InsertionRequest the StrainABE-0009478uacTygfU::Tn5KAN-I-SceI at position 536 in Plus orientation ,- pKD465Tim Durfee (durf@genome.wisc.edu)
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 3026367 to 3037815
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0009465guaDCDSforward30257663027085Upstream
ABE-0174113ghxQCDSforward30271213028488Upstream
ABE-0009471ygfSCDScomplement30285243029012Upstream
ABE-0009473ygfTCDScomplement30290123030931Upstream
ABE-0009478uacTCDSforward30313673032815Matches
ABE-0285227yqfGCDSforward30328173032942Downstream
ABE-0285740yqfHCDScomplement30329393033010Downstream
ABE-0009484idiCDSforward30330653033613Downstream
ABE-0009486lysSCDScomplement30336573035174Downstream
ABE-0009488prfBCDScomplement30351843036206(Interval #2) Downstream
ABE-0009488prfBCDScomplement30362083036282(Interval #1) Downstream
ABE-0009492recJCDScomplement30363733038106Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.