Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0009728 (nupG) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0009728 showshow history
Names
nupG JW2932 ECK2959 b2964
Product
nucleoside transporter
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 3105714 3106970 1257 419
Sequence

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View sequence with flanking regions: bases upstream and bases downstream

Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR007114 (IPR007114 (DEPRECATED) Major facilitator superfamily) Apr 2008
Automated Process Approved IPR004740 (IPR004740 Nucleoside:H+ symporter) Apr 2008
Automated Process Approved IPR036259 (IPR036259 MFS transporter superfamily) Jul 2018
Automated Process Approved PF03825 (PF03825 Nucleoside H+ symporter) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Uncurated GO biological process GO:0015949 nucleobase-containing small molecule interconversion Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0015858 nucleoside transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004740
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0006810 transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR007114
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO cellular component GO:0016021 integral component of membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR007114
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0005337 nucleoside transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004740
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0005215 transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR007114
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name nupG Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym ECK2959 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW2932 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b2964 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product nucleoside transporter Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved alternate product name transport of nucleosides, permease protein Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Person Uncurated alternate product name nucleoside transport protein (MFS family) Published Annotation - Database Name: GenProtEC Jan 2006
Protein Family
Automated Process Approved protein family IPR007114 (DEPRECATED) Major facilitator superfamily Protein Sequence Similarity - InterPro Domain: IPR007114
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR036259 MFS transporter superfamily Protein Sequence Similarity - InterPro Domain: IPR036259
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF03825 Nucleoside H+ symporter Protein Sequence Similarity - PFAM Model Name: PF03825
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR004740 Nucleoside:H+ symporter Protein Sequence Similarity - InterPro Domain: IPR004740
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Person Uncurated MultiFun 1.7.33 (metabolism; central intermediary metabolism; nucleotide and nucleoside conversions) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 4.S.147 (transport; substrate; nucleoside/H+) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 4.2.A.1 (transport; Electrochemical potential driven transporters; Porters (Uni-, Sym- and Antiporters); The Major Facilitator Superfamily (MFS)) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 6.1 (cell structure; membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 7.3 (location of gene products; inner membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG10664 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P0AFF4 Published Annotation - Swiss-Prot Accession Number: P0AFF4 Jan 2006
Automated Process Approved db xref GeneID:946282 Published Annotation - Entrez Gene Database ID: 946282
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref GI:87082192 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P0AFF4 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0009728 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved function transport; Transport of small molecules: Nucleosides, purines, pyrimidines Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note transport of nucleosides, permease protein; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0015949 - nucleobase, nucleoside and nucleotide interconversion Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC76001.2 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Membrane Predicted by PSORTb - PSORTb Final_Score: 10.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Person Uncurated GO biological process GO:0015949 nucleobase-containing small molecule interconversion Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0015858 nucleoside transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004740
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0006810 transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR007114
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO cellular component GO:0016021 integral component of membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR007114
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0005337 nucleoside transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004740
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0005215 transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR007114
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0006840 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0000386 xapB CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0001902 xapB CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0002903 nupG CDS
Ortholog
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0000729 CDS
Automated Process Approved Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0000786 nupG_1 CDS
Automated Process Uncurated Go view Citrobacter freundii ATCC 8090 [T] AVI-0004558 nupG_2 CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0004720 CDS
Automated Process Approved Go view Escherichia albertii TW07627 AFX-0002583 nupG CDS
Automated Process Approved Go view Escherichia coli 101-1 (EAEC) BAB-0005869 nupG CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0002988 CDS
Automated Process Approved Go view Escherichia coli 53638 (EIEC) ADP-0008175 nupG CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0002960 nupG CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0001310 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0006219 CDS
Automated Process Approved Go view Escherichia coli B171 (EPEC) ADN-0005664 nupG CDS
Automated Process Approved Go view Escherichia coli B7A (ETEC) ADK-0008279 CDS
Person Uncurated Go view Escherichia coli BL21(DE3) AHL-0005432 pseudogene
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0079713 nupG CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0003400 xapB CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0003923 CDS
Automated Process Approved Go view Escherichia coli E110019 (EPEC) ADM-0005705 nupG CDS
Automated Process Approved Go view Escherichia coli E22 (EPEC) ADL-0007284 nupG CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0003422 nupG CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0007746 nupG CDS
Automated Process Approved Go view Escherichia coli EC4024 (EHEC) BBI-0000225 CDS
Automated Process Approved Go view Escherichia coli EC4042 (EHEC) BBA-0007606 nupG CDS
Automated Process Approved Go view Escherichia coli EC4045 (EHEC) BBB-0006124 nupG CDS
Automated Process Approved Go view Escherichia coli EC4076 (EHEC) BAZ-0008382 nupG CDS
Automated Process Approved Go view Escherichia coli EC4113 (EHEC) BAY-0005716 nupG CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0009104 nupG CDS
Automated Process Approved Go view Escherichia coli EC4196 (EHEC) BAX-0006061 nupG CDS
Automated Process Approved Go view Escherichia coli EC4206 (EHEC) BAW-0006907 nupG CDS
Automated Process Approved Go view Escherichia coli EC4401 (EHEC) BAV-0005888 nupG CDS
Automated Process Approved Go view Escherichia coli EC4486 (EHEC) BAU-0006400 nupG CDS
Automated Process Approved Go view Escherichia coli EC4501 (EHEC) BAT-0005713 nupG CDS
Automated Process Approved Go view Escherichia coli EC508 (EHEC) BBJ-0005295 nupG CDS
Automated Process Approved Go view Escherichia coli EC869 (EHEC) BBC-0006140 nupG CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0027983 nupG CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0006886 nupG CDS
Automated Process Approved Go view Escherichia coli HS ADR-0007806 nupG CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0003447 CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0003035 nupG CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0003292 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0003423 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0003172 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0003296 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0177550 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0003294 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0003106 nupG CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0133883 nupG CDS
Automated Process Approved Go view Escherichia coli TW14588 (EHEC) BBK-0001141 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0003364 nupG CDS
Person Approved Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0003405 nupG CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0001601 CDS
Automated Process Approved Go view Salmonella Agona SL483 BBX-0007444 nupG CDS
Automated Process Approved Go view Salmonella Arizonae CDC 346-86 BCD-0004757 CDS
Person Approved Go view Salmonella Choleraesuis SC-B67 ADB-0003574 nupG CDS
Automated Process Approved Go view Salmonella Dublin CT_02021853 BCB-0007709 nupG CDS
Automated Process Approved Go view Salmonella Gallinarum 287/91 BCG-0009129 nupG CDS
Automated Process Approved Go view Salmonella Heidelberg SL476 BBV-0007713 nupG CDS
Automated Process Approved Go view Salmonella Javiana GA_MM04042433 BCA-0005670 nupG CDS
Automated Process Approved Go view Salmonella Kentucky CDC 191 BBZ-0008575 nupG CDS
Automated Process Approved Go view Salmonella Kentucky CVM29188 BBP-0006828 nupG CDS
Automated Process Approved Go view Salmonella Newport SL254 BBQ-0007720 nupG CDS
Automated Process Approved Go view Salmonella Newport SL317 BBR-0005351 nupG CDS
Automated Process Approved Go view Salmonella Paratyphi A AKU_12601 BCH-0003004 CDS
Person Approved Go view Salmonella Paratyphi A ATCC 9150 ADA-0002975 nupG CDS
Automated Process Approved Go view Salmonella Paratyphi B SPB7 BCC-0003896 CDS
Automated Process Approved Go view Salmonella Saintpaul SARA23 BBN-0007874 nupG CDS
Automated Process Approved Go view Salmonella Saintpaul SARA29 BBM-0007195 nupG CDS
Automated Process Approved Go view Salmonella Schwarzengrund CVM19633 BBO-0007359 nupG CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0111986 nupG CDS
Person Approved Go view Salmonella Typhi Ty2 ABS-0084745 nupG CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0099699 nupG CDS
Automated Process Approved Go view Salmonella Virchow SL491 BBS-0006140 nupG CDS
Automated Process Approved Go view Salmonella Weltevreden SL484 BBU-0006223 nupG CDS
Person Approved Go view Shigella boydii 227 ADV-0003319 nupG CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0008811 nupG CDS
Automated Process Approved Go view Shigella dysenteriae 1012 BAA-0003857 nupG CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0003547 nupG CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0074350 nupG CDS
Person Approved Go view Shigella flexneri 301 ABT-0089210 nupG CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0003222 nupG CDS
Person Approved Go view Shigella sonnei 046 ADD-0003532 nupG CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0007281 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0002466 CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB20994mutantTn5 InsertionRequest the StrainABE-0009728nupGnupG::Tn5KAN-I-SceI at position 723 in Minus orientation ,+ pKD460Blattner Laboratory
FB20995mutantTn5 InsertionRequest the StrainABE-0009728nupGnupG::Tn5KAN-I-SceI at position 723 in Minus orientation ,- pKD465Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 3100714 to 3111970
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0009700ansBCDScomplement30996823100728Upstream
ABE-0009706yggNCDScomplement31009043101623Upstream
ABE-0285564repeat_regionforward31016963101774Upstream
ABE-0009712yggLCDScomplement31018073102133Upstream
ABE-0009714trmBCDScomplement31021333102852Upstream
ABE-0009720mutYCDSforward31030133104065Upstream
ABE-0009722yggXCDSforward31040933104368Upstream
ABE-0009725mltCCDSforward31044333105512Upstream
ABE-0009728nupGCDSforward31057143106970Matches
ABE-0009730speCCDScomplement31070203109155Downstream
ABE-0285816yqgHCDScomplement31093173109400Downstream
ABE-0009736yqgACDSforward31095533110260Downstream
ABE-0009738pheVtRNAforward31103663110441Downstream
ABE-0009740yghDCDScomplement31105903111126Downstream
ABE-0009742yghEpseudogenecomplement31111283112092Downstream
ABE-0285801yqhJCDSforward31112663111325Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.