Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0009800 (pitB) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0009800 showshow history
Names
pitB JW2955 ECK2981 b2987
Product
phosphate transporter
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 3134872 3136371 1500 500
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR001204 (IPR001204 Phosphate transporter) Apr 2008
Automated Process Approved PF01384 (PF01384 Phosphate transporter family) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Uncurated GO biological process GO:0006793 phosphorus metabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0006817 phosphate ion transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001204
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001204
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0005315 inorganic phosphate transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001204
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name pitB Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym JW2955 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym ECK2981 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b2987 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product phosphate transporter Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved alternate product name low-affinity phosphate transport Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Person Uncurated alternate product name low-affinity phosphate transport protein (PiT family) Published Annotation - Database Name: GenProtEC Jan 2006
Protein Family
Automated Process Approved protein family IPR001204 Phosphate transporter Protein Sequence Similarity - InterPro Domain: IPR001204
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF01384 Phosphate transporter family Protein Sequence Similarity - PFAM Model Name: PF01384
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Person Uncurated MultiFun 1.8.1 (metabolism; metabolism of other compounds; phosphorous metabolism) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 4.2.A.20 (transport; Electrochemical potential driven transporters; Porters (Uni-, Sym- and Antiporters); The Inorganic Phosphate Transporter (PiT) Family) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 7.3 (location of gene products; inner membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 4.S.155 (transport; substrate; phosphate) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 6.1 (cell structure; membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:947475 Published Annotation - Entrez Gene Database ID: 947475
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Person Approved db xref UniProtKB:P43676 Published Annotation - Swiss-Prot Accession Number: P43676 Jan 2005
Automated Process Approved db xref GI:1789360 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P43676 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG12883 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0009800 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved function transport; Transport of small molecules: Anions Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note low-affinity phosphate transport; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0006793 - phosphorus metabolic process Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC76023.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Membrane Predicted by PSORTb - PSORTb Final_Score: 10.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Person Uncurated GO biological process GO:0006793 phosphorus metabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0006817 phosphate ion transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001204
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001204
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0005315 inorganic phosphate transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001204
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Homolog
Person Uncurated Go view Shewanella oneidensis MR-1 ACY-0264607 pit CDS
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0000119 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0000047 pitB CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0000052 pitB CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0000162 pitB CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0000049 pitB CDS
Ortholog
Automated Process Uncurated Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0001827 pitA_2 CDS
Person Approved Go view Escherichia coli 536 (UPEC) AEF-0003102 CDS
Person Approved Go view Escherichia coli APEC O1 (APEC) AEG-0003064 pitB CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0001201 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0006165 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0079895 pitB CDS
Person Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0003441 pitB pseudogene
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0004050 CDS
Automated Process Uncurated Go view Escherichia coli E2348/69 (EPEC) BCI-0003491 pitB CDS
Person Approved Go view Escherichia coli E24377A (ETEC) ADO-0007888 pitB CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0028014 pitB pseudogene
Automated Process Uncurated Go view Escherichia coli F11 (UPEC) ADQ-0007654 pitA_1 CDS
Person Approved Go view Escherichia coli HS ADR-0007849 pitB CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0003502 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0002788 pitB CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0003058 pitB CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0003601 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0003383 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0003551 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0003439 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0177609 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0003334 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0003270 pitB CDS
Person Uncurated Go view Escherichia coli Sakai (EHEC) ACA-0133929 pitB pseudogene
Person Approved Go view Escherichia coli UTI89 (UPEC) AEA-0003418 pitB CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0003522 pitB CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0074416 pitB CDS
Person Approved Go view Shigella flexneri 301 ABT-0089281 pitB CDS
Person Uncurated Go view Shigella flexneri 8401 BAG-0003241 pitB pseudogene
Person Approved Go view Shigella sonnei 046 ADD-0003423 pitB CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB21000mutantTn5 InsertionRequest the StrainABE-0009800pitBpitB::Tn5KAN-I-SceI at position 225 in Plus orientation ,+ pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 3129872 to 3141371
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0009779yghOpseudogenecomplement31290433130145Downstream
ABE-0009781is_elementcomplement31301463131340Downstream
ABE-0285567mobile_elementforward31301463131340Downstream
ABE-0009783insH9CDSforward31302143131194Downstream
ABE-0009786yghQpseudogenecomplement31313403132408Downstream
ABE-0285163yghQ_1CDSpcomplement31313413132408Downstream
ABE-0009789yghRCDScomplement31324543133212Downstream
ABE-0009793yghSCDScomplement31332443133957Downstream
ABE-0009798yghTCDSforward31341313134823Downstream
ABE-0009800pitBCDScomplement31348723136371Matches
ABE-0009804gssCDScomplement31366633138522Upstream
ABE-0009812yghUCDSforward31387273139593Upstream
ABE-0285568repeat_regionforward31396213139703Upstream
ABE-0009816hybGCDScomplement31397163139964Upstream
ABE-0009818hybFCDScomplement31399773140318Upstream
ABE-0009824hybECDScomplement31403113140799Upstream
ABE-0009826hybDCDScomplement31407923141286Upstream
ABE-0009828hybCCDScomplement31412863142989Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.