Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0009832 (hybA) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0009832 showshow history
Names
hybA JW2964 ECK2990 hydL hydC b2996
Product
hydrogenase 2 iron-sulfur protein
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 3144154 3145140 987 329
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR006311 (IPR006311 Twin-arginine translocation pathway, signal sequence) Apr 2008
Automated Process Approved IPR001450 (IPR001450 (DEPRECATED) 4Fe-4S binding domain) Apr 2008
Automated Process Approved IPR017896 (IPR017896 4Fe-4S ferredoxin-type, iron-sulphur binding domain) Jul 2018
Automated Process Approved IPR019546 (IPR019546 Twin-arginine translocation pathway, signal sequence, bacterial/archaeal) Jul 2018
Automated Process Approved PF13247 (PF13247 4Fe-4S dicluster domain) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0030288 outer membrane-bounded periplasmic space GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0051536 iron-sulfur cluster binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0009055 electron transfer activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name hybA Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym hydC Published Sequence Analysis - PubMed ID: 9729611 Aug 2003
Person Approved synonym JW2964 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym hydL Published Sequence Analysis - PubMed ID: 9729611 Aug 2003
Person Approved synonym ECK2990 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b2996 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product hydrogenase 2 iron-sulfur protein Published Annotation - EcoCyc Accession Number: EG11799
Reference: Escherichia coli K-12 MG1655 		Oct 2021
Person Approved alternate product name hydrogenase 2 4Fe-4S ferredoxin-type component Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved alternate product name hydrogenase-2 small subunit Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Person Uncurated alternate product name putative oxidoreductase, Fe-S protein Published Annotation - Database Name: GenProtEC Jan 2006
Protein Family
Automated Process Approved protein family IPR006311 Twin-arginine translocation pathway, signal sequence Protein Sequence Similarity - InterPro Domain: IPR006311
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR017896 4Fe-4S ferredoxin-type, iron-sulphur binding domain Protein Sequence Similarity - InterPro Domain: IPR017896
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF13247 4Fe-4S dicluster domain Protein Sequence Similarity - PFAM Model Name: PF13247
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR019546 Twin-arginine translocation pathway, signal sequence, bacterial/archaeal Protein Sequence Similarity - InterPro Domain: IPR019546
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR001450 (DEPRECATED) 4Fe-4S binding domain Protein Sequence Similarity - InterPro Domain: IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0009832 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG11799 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P0AAJ8 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:1789370 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:944842 Published Annotation - Entrez Gene Database ID: 944842
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Person Approved db xref UniProtKB:P0AAJ8 Published Annotation - Swiss-Prot Accession Number: P0AAJ8 Jan 2006
Person Approved function enzyme; Energy metabolism, carbon: Anaerobic respiration Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note hydrogenase-2 small subunit Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC76032.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Periplasmic Predicted by PSORTb - PSORTb Final_Score: 10.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0030288 outer membrane-bounded periplasmic space GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0009055 electron transfer activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0051536 iron-sulfur cluster binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001450
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0002840 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002778 hybA CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002825 hybA CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0008412 hybA CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0002674 hybA CDS
Ortholog
Automated Process Uncurated Go view Brenneria salicis ATCC 15712 [T] AMK-0001732 CDS
Automated Process Uncurated Go view Brenneria sp. EniD312 AKG-0001302 CDS
Automated Process Approved Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0000748 hybA CDS
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0004571 hybA CDS
Automated Process Uncurated Go view Dickeya chrysanthemi Ech1591 AFK-0006066 CDS
Person Approved Go view Dickeya dadantii 3937 ABF-0017348 hybA CDS
Automated Process Uncurated Go view Dickeya parazeae Ech586 AGC-0002807 CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0002497 hybA CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0005763 hybA CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0003112 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0008122 hybA CDS
Person Approved Go view Escherichia coli APEC O1 (APEC) AEG-0003072 hybA CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0001192 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0006155 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0005571 hybA CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0007842 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0079904 hybA CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0003450 CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0004062 CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0005657 hybA CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0005536 hybA CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0003500 hybA CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0007899 hybA CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0000266 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0007648 hybA CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0006165 hybA CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0008424 hybA CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0005676 hybA CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0009144 hybA CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0006020 hybA CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0006948 hybA CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0005929 hybA CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0006443 hybA CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0005752 hybA CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0005335 hybA CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0006180 hybA CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0028023 hybA CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0007645 fdnH_2 CDS
Automated Process Approved Go view Escherichia coli HS ADR-0007858 hybA CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0003511 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0002797 hybA CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0003067 hybA CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0003631 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0003374 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0003542 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0003448 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0177618 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0003343 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0003280 hybA CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0133938 hybA CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0005836 CDS
Person Approved Go view Escherichia coli UTI89 (UPEC) AEA-0003427 hybA CDS
Person Uncurated Go view Hafnia alvei ATCC 13337 [T] AMD-0003467 CDS
Automated Process Uncurated Go view Musicola paradisiaca Ech703 AGA-0007379 CDS
Person Approved Go view Pectobacterium atrosepticum SCRI1043 ABL-0061472 hybA CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0007489 hybA CDS
Automated Process Uncurated Go view Salmonella Arizonae CDC 346-86 BCD-0004700 CDS
Person Approved Go view Salmonella Choleraesuis SC-B67 ADB-0003612 hybA CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0007748 hybA CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0009757 hybA CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0009227 hybA CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0007356 hybA CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0007755 hybA CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0006715 hybA CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0005713 hybA CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0006177 hybA CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0006876 hybA CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0007760 hybA CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0005312 hybA CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0003049 CDS
Person Approved Go view Salmonella Paratyphi A ATCC 9150 ADA-0003016 hybA CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0003946 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0007914 hybA CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0007157 hybA CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0007402 hybA CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0007323 hybA CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0112058 hybA CDS
Person Approved Go view Salmonella Typhi Ty2 ABS-0084789 hybA CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0003819 hybA CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0099808 hybA CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0006087 hybA CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0006172 hybA CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0004723 hybA CDS
Person Approved Go view Shigella boydii 227 ADV-0003150 hybA CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0008837 hybA CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0004000 hybA CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0003513 hybA CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0074426 hybA CDS
Person Approved Go view Shigella flexneri 301 ABT-0089290 hybA CDS
Person Approved Go view Shigella flexneri 8401 BAG-0003250 hybA CDS
Person Approved Go view Shigella sonnei 046 ADD-0003432 hybA CDS
Person Uncurated Go view Yersinia bercovieri ATCC 43970 [T] AEK-0001702 hybA CDS
Person Uncurated Go view Yersinia enterocolitica 8081 BAH-0010356 hybA CDS
Person Uncurated Go view Yersinia frederiksenii ATCC 33641 [T] AEI-0002049 hybA CDS
Person Uncurated Go view Yersinia intermedia ATCC 29909 [T] AEH-0001857 hybA CDS
Person Uncurated Go view Yersinia mollaretii ATCC 43969 [T] AEJ-0002948 hybA CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0012871 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0004021 CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB21007mutantTn5 InsertionRequest the StrainABE-0009832hybAhybA::Tn5KAN-I-SceI at position 342 in Minus orientation ,- pKD465Blattner Laboratory
FB21008mutantTn5 InsertionRequest the StrainABE-0009832hybAhybA::Tn5KAN-I-SceI at position 342 in Minus orientation ,+ pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 3139154 to 3150140
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0009812yghUCDSforward31387273139593Downstream
ABE-0285568repeat_regionforward31396213139703Downstream
ABE-0009816hybGCDScomplement31397163139964Downstream
ABE-0009818hybFCDScomplement31399773140318Downstream
ABE-0009824hybECDScomplement31403113140799Downstream
ABE-0009826hybDCDScomplement31407923141286Downstream
ABE-0009828hybCCDScomplement31412863142989Downstream
ABE-0009830hybBCDScomplement31429863144164Overlaps Downstream
ABE-0009832hybACDScomplement31441543145140Matches
ABE-0009834hybOCDScomplement31451433146261Upstream
ABE-0009839yghWCDScomplement31464503146737Upstream
ABE-0009842yghX_3CDSpcomplement31468563147266Upstream
ABE-0285143yghXpseudogenecomplement31468563147740Upstream
ABE-0009844yghX_2CDSpcomplement31472663147619Upstream
ABE-0285762yqhICDSpcomplement31475973147740Upstream
ABE-0009848gprCDSforward31478973148937Upstream
ABE-0009850yqhACDScomplement31489773149471Upstream
ABE-0009859yghACDSforward31496623150546Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.