Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0010079 (nfeF) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0010079 showshow history
Names
nfeF JW3041 ECK3060 yqjH b3070
Product
NADPH-dependent ferric-chelate reductase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 3215727 3216491 765 255
Sequence

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View sequence with flanking regions: bases upstream and bases downstream

Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR013113 (IPR013113 FAD-binding 9, siderophore-interacting) Apr 2008
Automated Process Approved IPR007037 (IPR007037 Siderophore-interacting protein, C-terminal domain) Apr 2008
Automated Process Approved IPR039374 (IPR039374 Siderophore-interacting protein) Jul 2018
Automated Process Approved IPR017938 (IPR017938 Riboflavin synthase-like beta-barrel) Jul 2018
Automated Process Approved PF04954 (PF04954 Siderophore-interacting protein) Jul 2018
Automated Process Approved PF08021 (PF08021 Siderophore-interacting FAD-binding domain) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name nfeF Experimental - PubMed ID: 29723740
Reference: Escherichia coli 		Apr 2020
Person Approved synonym ECK3060 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym yqjH Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym JW3041 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b3070 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product NADPH-dependent ferric-chelate reductase Experimental - PubMed ID: 22098718
Reference: Escherichia coli K-12 		Feb 2021
Person Uncurated alternate product name putative ferric siderophore reductase Experimental - PubMed ID: 21097627
Reference: Escherichia coli K-12 MG1655 		Feb 2021
Person Uncurated alternate product name Ni-responsive Fe-uptake flavoprotein Experimental - PubMed ID: 29723740
Reference: Escherichia coli K-12 		Feb 2021
Person Uncurated alternate product name putative enzyme, ferredoxin reductase-like, FAD-linked, NADP-linked Published Annotation - Database Name: GenProtEC Jan 2006
Protein Family
Automated Process Approved protein family PF04954 Siderophore-interacting protein Protein Sequence Similarity - PFAM Model Name: PF04954
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR017938 Riboflavin synthase-like beta-barrel Protein Sequence Similarity - InterPro Domain: IPR017938
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF08021 Siderophore-interacting FAD-binding domain Protein Sequence Similarity - PFAM Model Name: PF08021
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR007037 Siderophore-interacting protein, C-terminal domain Protein Sequence Similarity - InterPro Domain: IPR007037
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR039374 Siderophore-interacting protein Protein Sequence Similarity - InterPro Domain: IPR039374
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR013113 FAD-binding 9, siderophore-interacting Protein Sequence Similarity - InterPro Domain: IPR013113
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:1789450 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:Q46871 Published Annotation - Swiss-Prot Accession Number: Q46871 Jan 2006
Automated Process Approved db xref GeneID:947582 Published Annotation - Entrez Gene Database ID: 947582
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref ASAP:ABE-0010079 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG12953 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC76105.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Predicted by PSORTb - PSORTb Final_Score: 8.96
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0002893 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002381 yqjH CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002228 yqjH CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0002319 yqjH CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0001975 nfeF CDS
Ortholog
Automated Process Uncurated Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0000668 yqjH CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0004555 CDS
Automated Process Uncurated Go view Erwinia tasmaniensis Et1/99 [T] AFI-0001420 yqjH CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0002381 CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0009165 CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0003190 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0008043 CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0003152 yqjH CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0001109 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0006072 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0007225 CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0007090 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0079995 nfeF CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0003533 CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0004158 CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0005610 CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0003584 yqjH CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0007969 CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0000342 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0007723 CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0006240 CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0007771 CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0007461 CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0009219 CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0005946 CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0007025 CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0006005 CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0008909 CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0007603 CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0005411 CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0006256 CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0028097 yqjH CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0007324 yqjH CDS
Automated Process Approved Go view Escherichia coli HS ADR-0007934 CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0003595 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0002866 yqjH CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0003139 yqjH CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0003709 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0003288 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0003462 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0003538 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0177708 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0003414 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0003362 CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0134010 yqjH CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0005912 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0003517 yqjH CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0003546 yqjH CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0001805 CDS
Person Uncurated Go view Pseudomonas syringae pv. tomato DC3000 ADG-0004266 CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0007567 CDS
Automated Process Uncurated Go view Salmonella Arizonae CDC 346-86 BCD-0004630 CDS
Person Uncurated Go view Salmonella Choleraesuis SC-B67 ADB-0003691 yqjH CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0007821 CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0009973 yqjH CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0009436 yqjH CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0007244 CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0007867 CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0006787 CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0005786 CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0006249 CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0006957 CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0007832 CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0005240 CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0003115 CDS
Person Uncurated Go view Salmonella Paratyphi A ATCC 9150 ADA-0003081 yqjH CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0004026 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0007987 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0008344 CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0007475 CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0007147 CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0112180 CDS
Person Uncurated Go view Salmonella Typhi Ty2 ABS-0084853 CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0003897 yqjH CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0099993 yqjH CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0006015 CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0006096 CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0004652 CDS
Person Uncurated Go view Shigella boydii 227 ADV-0003217 yqjH CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0008923 CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0004062 CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0003707 yqjH CDS
Person Uncurated Go view Shigella flexneri 2457T ABQ-0074506 yqjH pseudogene
Person Approved Go view Shigella sonnei 046 ADD-0003502 yqjH CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0006873 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0002656 CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB22515mutantTn5 InsertionRequest the StrainABE-0010079nfeFyqjH::Tn5KAN-I-SceI at position 154 in Minus orientation ,+ pKD460Blattner Laboratory
FB23284mutantTn5 InsertionRequest the StrainABE-0010079nfeFyqjH::Tn5KAN-I-SceI at position 154 in Minus orientation ,- pKD465Tim Durfee (durf@genome.wisc.edu)
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 3210727 to 3221491
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0010063rpsUCDSforward32107813210996Downstream
ABE-0010065dnaGCDSforward32111073212852Downstream
ABE-0010070rpoDCDSforward32130473214888Downstream
ABE-0285575repeat_regionforward32148923214924Downstream
ABE-0010073mugCDScomplement32149673215473Downstream
ABE-0010076ileXtRNAforward32155983215673Downstream
ABE-0010079nfeFCDScomplement32157273216491Matches
ABE-0285576repeat_regionforward32166013216678Upstream
ABE-0010084nfeRCDSforward32167793217402Upstream
ABE-0285577repeat_regionforward32174343217522Upstream
ABE-0010087aerCDScomplement32175563219076Upstream
ABE-0010092patACDSforward32194943220873Upstream
ABE-0285578repeat_regionforward32208803220910Upstream
ABE-0010094ygjHCDScomplement32209153221247Upstream
ABE-0010099ebgRCDSforward32214663222449Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.