Escherichia coli K-12 MG1655
Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0010307 (kbaY) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0010307 showshow history
Names
kbaY kba yraC agaY JW3106 ECK3125 b3137
Product
tagatose-1,6-bisphosphate aldolase 1
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 3283143 3284003 861 287
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR000771 (IPR000771 Fructose-bisphosphate aldolase, class-II) Apr 2008
Automated Process Approved IPR011288 (IPR011288 D-tagatose-bisphosphate aldolase, class II, subunit KbaY/GatY) Apr 2008
Automated Process Approved IPR013785 (IPR013785 Aldolase-type TIM barrel) Apr 2008
Automated Process Approved PF01116 (PF01116 Fructose-bisphosphate aldolase class-II) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Uncurated GO biological process GO:0005975 carbohydrate metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR000771, IPR011288
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Approved GO biological process GO:0008152 metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR013785
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Approved GO biological process GO:0019512 lactose catabolic process via tagatose-6-phosphate GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR011288
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Approved GO biological process GO:0006096 glycolytic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000771
Reference: Escherichia coli K-12 MG1655
Apr 2008
Person Uncurated GO biological process GO:0016052 carbohydrate catabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655
Feb 2008
Automated Process Uncurated GO molecular function GO:0009025 tagatose-bisphosphate aldolase activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR011288
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Approved GO molecular function GO:0003824 catalytic activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR013785
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Approved GO molecular function GO:0016832 aldehyde-lyase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR011288
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Approved GO molecular function GO:0008270 zinc ion binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000771
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Approved GO molecular function GO:0004332 fructose-bisphosphate aldolase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000771
Reference: Escherichia coli K-12 MG1655
Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name kbaY Published Annotation - PubMed ID: 16397293 Jan 2006
Automated Process Approved synonym yraC Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved synonym kba Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Person Approved synonym ECK3125 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym agaY Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655
Jan 2006
Person Approved synonym JW3106 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b3137 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product tagatose-1,6-bisphosphate aldolase 1 Published Annotation - EcoCyc Accession Number: EG12768
Reference: Escherichia coli K-12 MG1655
Oct 2021
Person Approved alternate product name tagatose-bisphosphate aldolase 2 Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655
Jan 2006
Person Uncurated alternate product name tagatose 6-phosphate aldolase 1, subunit together with AgaZ Published Annotation - Database Name: GenProtEC Jan 2006
Person Approved alternate product name tagatose 6-phosphate aldolase 1, kbaY subunit Published Annotation - PubMed ID: 16397293 Jan 2006
Protein Family
Automated Process Approved protein family PF01116 Fructose-bisphosphate aldolase class-II Protein Sequence Similarity - PFAM Model Name: PF01116
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Approved protein family IPR013785 Aldolase-type TIM barrel Protein Sequence Similarity - InterPro Domain: IPR013785
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Approved protein family IPR000771 Fructose-bisphosphate aldolase, class-II Protein Sequence Similarity - InterPro Domain: IPR000771
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Approved protein family IPR011288 D-tagatose-bisphosphate aldolase, class II, subunit KbaY/GatY Protein Sequence Similarity - InterPro Domain: IPR011288
Reference: Escherichia coli K-12 MG1655
Apr 2008
Other
Person Approved EC number 4.1.2.- Published Annotation - Swiss-Prot Accession Number: P0AB74 Feb 2006
Person Uncurated MultiFun 1.1.1 (metabolism; carbon utilization; carbon compounds) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref GI:1789526 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref EcoGene:EG12768 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref ASAP:ABE-0010307 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P0AB74 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref GeneID:947644 Published Annotation - Entrez Gene Database ID: 947644
Reference: Escherichia coli K-12 MG1655
Jun 2009
Person Approved db xref UniProtKB:P0AB74 Published Annotation - Swiss-Prot Accession Number: P0AB74 Jan 2006
Person Approved function enzyme; Central intermediary metabolism: Pool, multipurpose conversions Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655
Oct 1998
Automated Process Approved note tagatose-bisphosphate aldolase 2; GO_process: GO:0016052 - carbohydrate catabolic process Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved protein id AAC76171.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Predicted by PSORTb - PSORTb Final_Score: 8.96
Reference: Escherichia coli K-12 MG1655
Feb 2008
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655
Mar 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655
Jan 2009
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655
Feb 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0008152 metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR013785
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Approved GO biological process GO:0019512 lactose catabolic process via tagatose-6-phosphate GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR011288
Reference: Escherichia coli K-12 MG1655
Apr 2008
Person Uncurated GO biological process GO:0016052 carbohydrate catabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0006096 glycolytic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000771
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Uncurated GO biological process GO:0005975 carbohydrate metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR000771, IPR011288
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655
Feb 2008
Automated Process Approved GO molecular function GO:0008270 zinc ion binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000771
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Uncurated GO molecular function GO:0009025 tagatose-bisphosphate aldolase activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR011288
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Approved GO molecular function GO:0016832 aldehyde-lyase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR011288
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Approved GO molecular function GO:0003824 catalytic activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR013785
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Approved GO molecular function GO:0004332 fructose-bisphosphate aldolase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000771
Reference: Escherichia coli K-12 MG1655
Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Homolog
Person Uncurated Go view Shewanella oneidensis MR-1 ACY-0264488 fba CDS
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0002154 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0000059 kbaY CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0000109 kbaY CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0000101 kbaY CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0003230 kbaY CDS
Ortholog
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0000569 CDS
Automated Process Uncurated Go view Cedecea davisae ATCC 33431 [T] ALZ-0003754 CDS
Automated Process Uncurated Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0000609 kbaY_1 CDS
Automated Process Uncurated Go view Citrobacter freundii ATCC 8090 [T] AVI-0004796 kbaY_2 CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0004483 CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0002296 agaY CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0008072 agaY CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0003259 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0007968 agaY CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0003208 kbaY CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0001050 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0005991 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0007300 agaY CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0009712 pseudogene
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0080066 kbaY CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0003609 CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0004233 CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0005501 agaY CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0005685 agaY CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0003647 kbaY CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0008051 agaY CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0002816 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0007794 agaY CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0006310 agaY CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0007841 agaY CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0007389 agaY CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0009290 agaY CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0005874 agaY CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0007095 agaY CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0006076 agaY CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0008980 agaY CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0007673 agaY CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0005482 agaY CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0006327 agaY CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0028165 kbaY CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0007252 kbaY_1 CDS
Automated Process Approved Go view Escherichia coli HS ADR-0008014 agaY CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0003655 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0002927 kbaY CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0003205 kbaY CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0003786 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0003709 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0003746 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0003607 kbaY CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0177766 gatY CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0003486 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0003434 agaY CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0134076 kbaY CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0005984 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0003577 kbaY CDS
Automated Process Uncurated Go view Ewingella americana ATCC 33852 [T] AMA-0001877 CDS
Automated Process Uncurated Go view Pantoea sp. At-9b AGM-0009641 CDS
Automated Process Uncurated Go view Pectobacterium parmentieri WPP163 AFJ-0003380 CDS
Automated Process Uncurated Go view Salmonella Arizonae CDC 346-86 BCD-0004574 CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0006414 agaY CDS
Automated Process Uncurated Go view Yersinia frederiksenii ATCC 33641 [T] AEI-0003822 kbaY_2 CDS
Automated Process Uncurated Go view Yersinia intermedia ATCC 29909 [T] AEH-0003744 kbaY CDS
Person Uncurated Go view Yersinia pestis 91001 (biovar Microtus) ACQ-0242851 agaY CDS
Automated Process Uncurated Go view Yersinia pestis Angola (biovar Pestoides) ADI-0007486 CDS
Automated Process Uncurated Go view Yersinia pestis Antiqua (biovar Antiqua) BAE-0000527 agaY CDS
Automated Process Uncurated Go view Yersinia pestis B42003004 (biovar Antiqua) BAL-0006811 CDS
Automated Process Uncurated Go view Yersinia pestis CA88-4125 (biovar Orientalis) BAF-0002499 agaY CDS
Person Uncurated Go view Yersinia pestis CO92 (biovar Orientalis) ABY-0118095 agaY CDS
Automated Process Uncurated Go view Yersinia pestis E1979001 (biovar Antiqua) BAM-0006625 CDS
Automated Process Uncurated Go view Yersinia pestis F1991016 (biovar Orientalis) BAQ-0005004 CDS
Automated Process Uncurated Go view Yersinia pestis FV-1 (biovar Orientalis) BAK-0000800 CDS
Automated Process Uncurated Go view Yersinia pestis IP275 (biovar Orientalis) BAC-0006487 CDS
Automated Process Uncurated Go view Yersinia pestis K1973002 (biovar Mediaevalis) BAO-0006510 CDS
Person Uncurated Go view Yersinia pestis KIM (biovar Mediaevalis) ABI-0032995 agaY CDS
Automated Process Uncurated Go view Yersinia pestis MG05-1020 (biovar Orientalis) BAP-0004557 CDS
Automated Process Uncurated Go view Yersinia pestis Nepal516 (biovar Antiqua) BAD-0003664 agaY CDS
Automated Process Uncurated Go view Yersinia pestis Pestoides F (biovar Pestoides) BAI-0000795 CDS
Automated Process Uncurated Go view Yersinia pestis UG05-0454 (biovar Antiqua) BAN-0006158 CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis IP31758 ADS-0004875 CDS
Person Uncurated Go view Yersinia pseudotuberculosis IP32953 ACZ-0003194 agaY CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis PB1/+ AFV-0003838 CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis YPIII AFW-0001146 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0006612 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0002725 CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)

Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB21038mutantTn5 InsertionRequest the StrainABE-0010307kbaYagaY::Tn5KAN-I-SceI at position 191 in Minus orientation ,- pKD465Blattner Laboratory
FB21039mutantTn5 InsertionRequest the StrainABE-0010307kbaYagaY::Tn5KAN-I-SceI at position 191 in Minus orientation ,+ pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 3278143 to 3289003
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0010291agaRCDScomplement32778563278665Upstream
ABE-0010295kbaZCDSforward32789143280194Upstream
ABE-0010297agaVCDSforward32802173280690Upstream
ABE-0010299agaWpseudogeneforward32807013281076Upstream
ABE-0285164agaW_1CDSpforward32807013281102Upstream
ABE-0010302agaApseudogeneforward32810713281625Upstream
ABE-0285587repeat_regionforward32817023281783Upstream
ABE-0010305agaSCDSforward32819763283130Upstream
ABE-0010307kbaYCDSforward32831433284003Matches
ABE-0010310agaBCDSforward32841703284646Downstream
ABE-0010312agaCCDSforward32846853285488Downstream
ABE-0010314agaDCDSforward32854783286269Downstream
ABE-0010317agaICDSforward32862703287025Downstream
ABE-0010325yraHCDSforward32874263288010Downstream
ABE-0010331yraICDSforward32880903288785Downstream
ABE-0010333yraJCDSforward32888143291330Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.