Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0010508 (lptC) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0010508 showshow history
Names
lptC yrbK JW3166 ECK3188 b3199
Product
lipopolysaccharide transport protein
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 3342836 3343411 576 192
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Person Approved ADX-0001182
EnteroFam0010508: Trusted threshold=426.6; Members of this family are defined by searches with an hmm constructed from an alignment of putative orthologous proteins from eight genomes (E. coli MG1655, E. coli EDL933, S. enterica Typhimurium LT2, S. enterica Typhi CT18, Y. pestis CO92, Y. pseudotuberculosis 32953, E. chrysanthemi 3937 and E. carotovora atroseptica SCRI1043). 		Jan 2006
Automated Process Approved IPR010664 (IPR010664 Lipopolysaccharide assembly, LptC-related) Apr 2008
Automated Process Approved IPR026265 (IPR026265 Lipopolysaccharide export system protein LptC) Jul 2018
Automated Process Approved PF06835 (PF06835 Lipopolysaccharide-assembly, LptC-related) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Uncurated GO biological process GO:0015920 lipopolysaccharide transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR026265
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated GO cellular component GO:0005887 integral component of plasma membrane GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR026265
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated GO molecular function GO:0015221 lipopolysaccharide transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR026265
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Uncurated name lptC Published Annotation - PubMed ID: 18424520
Reference: Escherichia coli K-12 		Mar 2009
Person Approved synonym ECK3188 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW3166 Published Annotation - PubMed ID: 16397293 Jan 2006
Automated Process Approved synonym yrbK Published Annotation - PubMed ID: 16397293
Reference: Escherichia coli K-12 MG1655 		Sep 2007
Person Approved locus tag b3199 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product lipopolysaccharide transport protein Experimental - PubMed ID: 18424520
Reference: Escherichia coli K-12 		Mar 2009
Automated Process Approved alternate product name lipopolysaccharide export, IM-tethered periplasmic protein of the LptBFGC export complex Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Protein Family
Person Approved EnteroFam EnteroFam0010508: Trusted threshold=426.6; Members of this family are defined by searches with an hmm constructed from an alignment of putative orthologous proteins from eight genomes (E. coli MG1655, E. coli EDL933, S. enterica Typhimurium LT2, S. enterica Typhi CT18, Y. pestis CO92, Y. pseudotuberculosis 32953, E. chrysanthemi 3937 and E. carotovora atroseptica SCRI1043). Protein Sequence Similarity - ASAP FeatureID: ADX-0001182
Reference: EnteroFams 		Jan 2006
Automated Process Approved protein family IPR010664 Lipopolysaccharide assembly, LptC-related Protein Sequence Similarity - InterPro Domain: IPR010664
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR026265 Lipopolysaccharide export system protein LptC Protein Sequence Similarity - InterPro Domain: IPR026265
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF06835 Lipopolysaccharide-assembly, LptC-related Protein Sequence Similarity - PFAM Model Name: PF06835
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG12806 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:1789591 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:947722 Published Annotation - Entrez Gene Database ID: 947722
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Person Approved db xref UniProtKB:P0ADV9 Published Annotation - Swiss-Prot Accession Number: P0ADV9 Jan 2006
Automated Process Approved db xref ASAP:ABE-0010508 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P0ADV9 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC76231.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Unknown Predicted by PSORTb - PSORTb Final_Score: 2.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Uncurated GO biological process GO:0015920 lipopolysaccharide transport GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR026265
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated GO cellular component GO:0005887 integral component of plasma membrane GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR026265
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated GO molecular function GO:0015221 lipopolysaccharide transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR026265
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Person Approved Go view EnteroFams ADX-0001182 lptC CDS
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0001206 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0001512 lptC CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0001217 lptC CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0000142 lptC CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0001373 lptC CDS
Ortholog
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0003883 lptC CDS
Automated Process Uncurated Go view Cedecea davisae ATCC 33431 [T] ALZ-0000069 CDS
Automated Process Approved Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0000548 lptC CDS
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0000054 lptC CDS
Automated Process Uncurated Go view Dickeya chrysanthemi Ech1591 AFK-0008951 CDS
Person Approved Go view Dickeya dadantii 3937 ABF-0015678 lptC CDS
Automated Process Uncurated Go view Dickeya parazeae Ech586 AGC-0000299 CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0004429 CDS
Automated Process Uncurated Go view Erwinia amylovora ATCC 49946 ACW-0305918 CDS
Automated Process Uncurated Go view Erwinia amylovora ATCC BAA-2158 APN-0000404 yrbK CDS
Automated Process Uncurated Go view Erwinia tasmaniensis Et1/99 [T] AFI-0000351 yrbK CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0002238 CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0008496 CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0003319 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0007899 CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0003264 CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0000994 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0005913 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0008786 CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0009052 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0080133 yrbK CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0003677 lptC CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0004297 yrbK CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0008908 CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0009177 CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0003706 yrbK CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0008119 CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0002517 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0007861 CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0006376 CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0007908 CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0009713 CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0009357 CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0009822 CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0007166 CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0006145 CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0008504 CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0009294 CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0008626 CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0006395 CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0028232 yrbK CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0009807 lptC CDS
Automated Process Approved Go view Escherichia coli HS ADR-0008079 CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0003712 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0002986 yrbK CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0003268 yrbK CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0003847 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0003881 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0003685 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0003673 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0177830 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0003548 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0003493 CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0134142 yrbK CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0006049 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0003644 yrbK CDS
Automated Process Uncurated Go view Ewingella americana ATCC 33852 [T] AMA-0000070 CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0003656 yrbK CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0001913 CDS
Automated Process Uncurated Go view Musicola paradisiaca Ech703 AGA-0008713 CDS
Automated Process Uncurated Go view Pantoea agglomerans IG1 APM-0003498 CDS
Automated Process Uncurated Go view Pantoea agglomerans SL1-M5 ALY-0002235 CDS
Automated Process Uncurated Go view Pantoea ananatis AJ13355 AOV-0003670 yrbK CDS
Automated Process Uncurated Go view Pantoea ananatis B1-9 APL-0004489 CDS
Automated Process Uncurated Go view Pantoea ananatis LMG 20103 AKB-0000451 yrbK CDS
Automated Process Approved Go view Pantoea ananatis PA13 APK-0003835 CDS
Automated Process Uncurated Go view Pantoea sp. At-9b AGM-0008231 CDS
Automated Process Approved Go view Pantoea stewartii DC283 ACV-0289082 CDS
Automated Process Uncurated Go view Pantoea vagans C9-1 ALK-0007395 yrbK CDS
Person Approved Go view Pectobacterium atrosepticum SCRI1043 ABL-0060509 yrbK CDS
Automated Process Uncurated Go view Pectobacterium brasiliensis 1692 AED-0000207 CDS
Person Uncurated Go view Pectobacterium carotovorum WPP14 ADT-0002386 CDS
Automated Process Uncurated Go view Pectobacterium parmentieri WPP163 AFJ-0000056 yrbK CDS
Automated Process Uncurated Go view Pectobacterium sp. PC1 AFL-0004777 CDS
Person Uncurated Go view Photorhabdus luminescens subsp. laumondii TTO1 [T] ACD-0146026 CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0007674 CDS
Automated Process Uncurated Go view Salmonella Arizonae CDC 346-86 BCD-0004509 CDS
Person Approved Go view Salmonella Choleraesuis SC-B67 ADB-0003802 yrbK CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0007927 CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0010282 yrbK CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0009731 yrbK CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0007134 CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0007973 CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0006892 CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0008448 CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0006358 CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0007069 CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0007935 CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0005130 CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0003217 CDS
Person Approved Go view Salmonella Paratyphi A ATCC 9150 ADA-0003183 yrbK CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0004152 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0008097 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0007234 CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0007580 CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0007251 CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0112398 yrbK CDS
Person Approved Go view Salmonella Typhi Ty2 ABS-0084954 yrbK CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0004011 yrbK CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0100293 yrbK CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0005912 CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0005993 CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0008566 CDS
Automated Process Uncurated Go view Serratia marcescens subsp. marcescens ATCC 13880 [T] AMF-0001484 CDS
Person Uncurated Go view Shewanella oneidensis MR-1 ACY-0267601 CDS
Person Approved Go view Shigella boydii 227 ADV-0003482 yrbK CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0008988 CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0007322 CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0003845 yrbK CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0074656 yrbK CDS
Person Approved Go view Shigella flexneri 301 ABT-0089478 yrbK CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0003436 yrbK CDS
Person Approved Go view Shigella sonnei 046 ADD-0003655 yrbK CDS
Person Uncurated Go view Trabulsiella guamensis ATCC 49490 [T] APH-0004007 lptC CDS
Person Uncurated Go view Wigglesworthia morsitans AEQ-0000496 lptC CDS
Person Uncurated Go view Xenorhabdus nematophila ATCC 19061 [T] ADW-0007538 CDS
Automated Process Uncurated Go view Yersinia bercovieri ATCC 43970 [T] AEK-0002310 CDS
Automated Process Uncurated Go view Yersinia enterocolitica 8081 BAH-0010795 yrbK CDS
Automated Process Uncurated Go view Yersinia frederiksenii ATCC 33641 [T] AEI-0002736 lptC CDS
Automated Process Uncurated Go view Yersinia intermedia ATCC 29909 [T] AEH-0001385 yrbK CDS
Automated Process Uncurated Go view Yersinia mollaretii ATCC 43969 [T] AEJ-0000162 CDS
Person Approved Go view Yersinia pestis 91001 (biovar Microtus) ACQ-0243506 yrbK CDS
Automated Process Uncurated Go view Yersinia pestis Antiqua (biovar Antiqua) BAE-0004442 yrbK CDS
Automated Process Uncurated Go view Yersinia pestis CA88-4125 (biovar Orientalis) BAF-0003755 CDS
Person Approved Go view Yersinia pestis CO92 (biovar Orientalis) ABY-0127350 yrbK CDS
Automated Process Uncurated Go view Yersinia pestis FV-1 (biovar Orientalis) BAK-0001423 CDS
Person Approved Go view Yersinia pestis KIM (biovar Mediaevalis) ABI-0029740 yrbK CDS
Automated Process Uncurated Go view Yersinia pestis Nepal516 (biovar Antiqua) BAD-0004160 yrbK CDS
Automated Process Uncurated Go view Yersinia pestis Pestoides F (biovar Pestoides) BAI-0000370 CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis IP31758 ADS-0004383 CDS
Person Approved Go view Yersinia pseudotuberculosis IP32953 ACZ-0003635 yrbK CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis PB1/+ AFV-0004421 CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis YPIII AFW-0000590 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0006348 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0002784 CDS
Molecular Interactions to toptop
Author Approval Feature Name Technique Evidence Comment Annotated
Person Approved ABE-0010508 lptC lambda repressor assay Doodle - bnumber: b3199 Oct 2003
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 3337836 to 3348411
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0010489mlaCCDScomplement33372563337891Upstream
ABE-0010493mlaDCDScomplement33379103338461Upstream
ABE-0010495mlaECDScomplement33384663339248Upstream
ABE-0010498mlaFCDScomplement33392563340065Upstream
ABE-0010502yrbGCDSforward33402753341252Upstream
ABE-0010504kdsDCDSforward33412663342252Upstream
ABE-0010506kdsCCDSforward33422733342839Overlaps Upstream
ABE-0010508lptCCDSforward33428363343411Matches
ABE-0010510lptACDSforward33433803343937Overlaps Downstream
ABE-0010512lptBCDSforward33439443344669Downstream
ABE-0010514rpoNCDSforward33447173346150Downstream
ABE-0010516hpfCDSforward33461733346460Downstream
ABE-0010520ptsNCDSforward33465783347069Downstream
ABE-0010522rapZCDSforward33471153347969Downstream
ABE-0010524nprCDSforward33479663348238Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.