Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0010547 (gltD) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0010547 showshow history
Names
gltD JW3180 psiQ ECK3203 ossB aspB b3213
Product
glutamate synthase small subunit
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 3359198 3360616 1419 473
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR001327 (IPR001327 (DEPRECATED) Pyridine nucleotide-disulphide oxidoreductase, NAD-binding domain) Apr 2008
Automated Process Approved IPR006006 (IPR006006 Glutamate synthase NADPH small chain-like) Apr 2008
Automated Process Approved IPR013027 (IPR013027 (DEPRECATED) FAD-dependent pyridine nucleotide-disulphide oxidoreductase) Apr 2008
Automated Process Approved IPR009051 (IPR009051 Alpha-helical ferredoxin) Apr 2008
Automated Process Approved IPR000759 (IPR000759 (DEPRECATED) Adrenodoxin reductase) Apr 2008
Automated Process Approved IPR000103 (IPR000103 (DEPRECATED) Pyridine nucleotide-disulphide oxidoreductase, class-II) Apr 2008
Automated Process Approved IPR012285 (IPR012285 (DEPRECATED) Fumarate reductase, C-terminal) Apr 2008
Automated Process Approved IPR023753 (IPR023753 FAD/NAD(P)-binding domain) Jul 2018
Automated Process Approved IPR028261 (IPR028261 Dihydroprymidine dehydrogenase domain II) Jul 2018
Automated Process Approved PF07992 (PF07992 Pyridine nucleotide-disulphide oxidoreductase) Jul 2018
Automated Process Approved PF14691 (PF14691 Dihydroprymidine dehydrogenase domain II, 4Fe-4S cluster) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Uncurated GO biological process GO:0055114 (OBSOLETE) oxidation-reduction process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR023753
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Approved GO biological process GO:0006537 glutamate biosynthetic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000759
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0006537 glutamate biosynthetic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006006
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO biological process GO:0006807 nitrogen compound metabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR012285
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0051536 iron-sulfur cluster binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR012285
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0016639 oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006006
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0050660 flavin adenine dinucleotide binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001327
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0016491 oxidoreductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001327
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name gltD Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved synonym psiQ Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved synonym JW3180 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym ECK3203 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym ossB Published Sequence Analysis - PubMed ID: 9729611 Aug 2003
Person Approved synonym aspB Published Sequence Analysis - PubMed ID: 9729611 Aug 2003
Person Approved locus tag b3213 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product glutamate synthase small subunit Published Annotation - PubMed ID: 16397293
Reference: Escherichia coli K-12 		Oct 2021
Person Uncurated alternate product name glutamate synthase, small subunit, nucleotide-binding, 4Fe-4S protein Published Annotation - Database Name: GenProtEC Jan 2006
Person Approved alternate product name glutamate synthase, small subunit Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Protein Family
Automated Process Approved protein family IPR001327 (DEPRECATED) Pyridine nucleotide-disulphide oxidoreductase, NAD-binding domain Protein Sequence Similarity - InterPro Domain: IPR001327
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF14691 Dihydroprymidine dehydrogenase domain II, 4Fe-4S cluster Protein Sequence Similarity - PFAM Model Name: PF14691
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR012285 (DEPRECATED) Fumarate reductase, C-terminal Protein Sequence Similarity - InterPro Domain: IPR012285
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR023753 FAD/NAD(P)-binding domain Protein Sequence Similarity - InterPro Domain: IPR023753
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR028261 Dihydroprymidine dehydrogenase domain II Protein Sequence Similarity - InterPro Domain: IPR028261
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF07992 Pyridine nucleotide-disulphide oxidoreductase Protein Sequence Similarity - PFAM Model Name: PF07992
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR000103 (DEPRECATED) Pyridine nucleotide-disulphide oxidoreductase, class-II Protein Sequence Similarity - InterPro Domain: IPR000103
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR000759 (DEPRECATED) Adrenodoxin reductase Protein Sequence Similarity - InterPro Domain: IPR000759
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR006006 Glutamate synthase NADPH small chain-like Protein Sequence Similarity - InterPro Domain: IPR006006
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR013027 (DEPRECATED) FAD-dependent pyridine nucleotide-disulphide oxidoreductase Protein Sequence Similarity - InterPro Domain: IPR013027
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR009051 Alpha-helical ferredoxin Protein Sequence Similarity - InterPro Domain: IPR009051
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Automated Process Uncurated EC number 1.4.1.- Published Annotation - Database Name: EC2GO: GO:0016639
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated EC number 1.-.-.- Published Annotation - Database Name: EC2GO: GO:0016491
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Approved EC number 1.4.1.13 Published Annotation - Swiss-Prot Accession Number: P09832 Feb 2006
Person Uncurated MultiFun 1.8.3 (metabolism; metabolism of other compounds; nitrogen metabolism) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 1.5.1.1 (metabolism; building block biosynthesis; amino acids; glutamate) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:947723 Published Annotation - Entrez Gene Database ID: 947723
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Person Approved db xref UniProtKB:P09832 Published Annotation - Swiss-Prot Accession Number: P09832 Jan 2005
Automated Process Approved db xref EcoGene:EG10404 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:1789606 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0010547 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P09832 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved function enzyme; Central intermediary metabolism: Pool, multipurpose conversions Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note glutamate synthase, small subunit; GO_process: GO:0006537 - glutamate biosynthetic process; GO_process: GO:0006807 - nitrogen compound metabolic process Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC76245.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Predicted by PSORTb - PSORTb Final_Score: 10.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Uncurated GO biological process GO:0055114 (OBSOLETE) oxidation-reduction process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR023753
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Approved GO biological process GO:0006537 glutamate biosynthetic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0006118 (OBSOLETE) electron transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000759
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0006537 glutamate biosynthetic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006006
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO biological process GO:0006807 nitrogen compound metabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR012285
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0051536 iron-sulfur cluster binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR012285
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0016639 oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR006006
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0050660 flavin adenine dinucleotide binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001327
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0016491 oxidoreductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001327
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0000012 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0000016 ygfT CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0000019 aegA CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0000099 aegA CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0000009 aegA CDS
Ortholog
Automated Process Uncurated Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0000533 gltD_1 CDS
Automated Process Uncurated Go view Citrobacter freundii ATCC 8090 [T] AVI-0000068 gltD_1 CDS
Person Approved Go view Dickeya dadantii 3937 ABF-0015710 gltD CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0003334 CDS
Automated Process Uncurated Go view Escherichia coli APEC O1 (APEC) AEG-0003278 gltD CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0000979 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0005896 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0080148 gltD CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0003693 gltD CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0004312 CDS
Automated Process Uncurated Go view Escherichia coli E2348/69 (EPEC) BCI-0003721 gltD CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0008133 gltD CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0009372 gltD CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0028246 gltD CDS
Automated Process Uncurated Go view Escherichia coli F11 (UPEC) ADQ-0009736 gltD_3 CDS
Automated Process Approved Go view Escherichia coli HS ADR-0008094 gltD CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0003000 gltD CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0003283 gltD CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0003863 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0003865 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0003669 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0003688 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0177845 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0003563 CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0134156 gltD CDS
Person Approved Go view Escherichia coli UTI89 (UPEC) AEA-0003659 gltD CDS
Person Approved Go view Pseudomonas syringae pv. tomato DC3000 ADG-0005199 gltD CDS
Person Uncurated Go view Salmonella Choleraesuis SC-B67 ADB-0003816 gltD CDS
Person Uncurated Go view Salmonella Paratyphi A ATCC 9150 ADA-0003198 gltD CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0112432 gltD CDS
Person Uncurated Go view Salmonella Typhi Ty2 ABS-0084968 gltD CDS
Person Uncurated Go view Salmonella Typhimurium LT2 [T] ABU-0100338 gltD CDS
Person Uncurated Go view Shewanella oneidensis MR-1 ACY-0264890 gltD CDS
Person Approved Go view Shigella boydii 227 ADV-0003468 gltD CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0008973 gltD CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0003859 gltD CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0074670 gltD CDS
Person Approved Go view Shigella flexneri 301 ABT-0089491 gltD CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0003450 gltD CDS
Person Approved Go view Shigella sonnei 046 ADD-0003669 gltD CDS
Person Uncurated Go view Xenorhabdus nematophila ATCC 19061 [T] ADW-0007493 gltD CDS
Person Uncurated Go view Yersinia pestis 91001 (biovar Microtus) ACQ-0243472 gltD2 CDS
Person Uncurated Go view Yersinia pestis Antiqua (biovar Antiqua) BAE-0004468 gltD CDS
Person Uncurated Go view Yersinia pestis CO92 (biovar Orientalis) ABY-0127283 gltD CDS
Person Uncurated Go view Yersinia pestis KIM (biovar Mediaevalis) ABI-0029717 gltD CDS
Person Uncurated Go view Yersinia pestis Nepal516 (biovar Antiqua) BAD-0004135 gltD CDS
Person Uncurated Go view Yersinia pseudotuberculosis IP32953 ACZ-0003615 gltD CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB21067mutantTn5 InsertionRequest the StrainABE-0010547gltDgltD::Tn5KAN-I-SceI at position 415 in Plus orientation ,+ pKD460Blattner Laboratory
FB21068mutantTn5 InsertionRequest the StrainABE-0010547gltDgltD::Tn5KAN-I-SceI at position 415 in Plus orientation ,- pKD465Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 3354198 to 3365616
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0010545gltBCDSforward33547253359185Upstream
ABE-0010547gltDCDSforward33591983360616Matches
ABE-0285593repeat_regionforward33606883360786Downstream
ABE-0010549gltFCDSforward33611763361940Downstream
ABE-0010552yhcACDSforward33621123362786Downstream
ABE-0010554yhcDCDSforward33628073365188Downstream
ABE-0010556yhcE_1CDSpforward33651853365664Downstream
ABE-0285102yhcEpseudogeneforward33651853365555(Interval #1) Downstream
ABE-0010557is_elementforward33655563366750Downstream
ABE-0285594mobile_elementcomplement33655563366750Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.