Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0010579 (nanA) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0010579 showshow history
Names
nanA npl JW3194 ECK3214 b3225
Product
N-acetylneuraminate lyase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 3372683 3373576 894 298
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR002220 (IPR002220 DapA-like) Apr 2008
Automated Process Approved IPR005264 (IPR005264 N-acetylneuraminate lyase) Apr 2008
Automated Process Approved IPR013785 (IPR013785 Aldolase-type TIM barrel) Apr 2008
Automated Process Approved PF00701 (PF00701 Dihydrodipicolinate synthetase family) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Uncurated GO biological process GO:0005975 carbohydrate metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR005264
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO biological process GO:0008152 metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002220
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Approved GO biological process GO:0046349 amino sugar biosynthetic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR005264
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0003824 catalytic activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR013785
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0016829 lyase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002220
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Approved GO molecular function GO:0008747 N-acetylneuraminate lyase activity Experimental - PubMed ID: 6389524
Reference: Escherichia coli 		Mar 2006
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Uncurated name nanA Published Annotation - PubMed ID: 3902799
Reference: Escherichia coli K-12 MG1655 		May 2009
Person Approved synonym JW3194 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Uncurated synonym npl Published Sequence Analysis - PubMed ID: 3909108
Reference: Escherichia coli K-12 		May 2009
Person Approved synonym ECK3214 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b3225 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product N-acetylneuraminate lyase Published Annotation - PubMed ID: 16397293 Jan 2006
Protein Family
Automated Process Approved protein family IPR002220 DapA-like Protein Sequence Similarity - InterPro Domain: IPR002220
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR013785 Aldolase-type TIM barrel Protein Sequence Similarity - InterPro Domain: IPR013785
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF00701 Dihydrodipicolinate synthetase family Protein Sequence Similarity - PFAM Model Name: PF00701
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR005264 N-acetylneuraminate lyase Protein Sequence Similarity - InterPro Domain: IPR005264
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Person Approved EC number 4.1.3.3 Published Annotation - Swiss-Prot Accession Number: P0A6L4 Feb 2006
Automated Process Uncurated EC number 4.-.-.- Published Annotation - Database Name: EC2GO: GO:0016829
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Uncurated MultiFun 1.7.12 (metabolism; central intermediary metabolism; amino sugar conversions) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 6.3 (cell structure; surface antigens (ECA, O antigen of LPS)) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG10637 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P0A6L4 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0010579 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P0A6L4 Published Annotation - Swiss-Prot Accession Number: P0A6L4 Jan 2006
Automated Process Approved db xref GeneID:947742 Published Annotation - Entrez Gene Database ID: 947742
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Person Approved db xref NCBI_gi:1789620 Published Annotation - GenPept Accession Number: AAC76257
Reference: Escherichia coli MG1655 		Mar 2006
Automated Process Approved db xref GI:1789620 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved function catabolism of sialic acid Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Mar 2006
Person Approved function enzyme; Surface polysaccharides & antigens Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note N-acetylneuraminate lyase (aldolase); catabolism of sialic acid; not K-12?; GO_function: GO:0042280 - cell surface antigen activity, host-interacting; GO_process: GO:0046349 - amino sugar biosynthetic process Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC76257.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Unknown Predicted by PSORTb - PSORTb Final_Score: 2.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Person Approved GO biological process GO:0046349 amino sugar biosynthetic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0008152 metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002220
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO biological process GO:0005975 carbohydrate metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR005264
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR005264
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0016829 lyase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR002220
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0003824 catalytic activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR013785
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Approved GO molecular function GO:0008747 N-acetylneuraminate lyase activity Experimental - PubMed ID: 6389524
Reference: Escherichia coli 		Mar 2006
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0002285 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002310 nanA CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002507 nanA CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0008593 nanA CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0002972 nanA CDS
Ortholog
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0003904 nanA CDS
Automated Process Uncurated Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0000527 nanA CDS
Automated Process Uncurated Go view Citrobacter freundii ATCC 8090 [T] AVI-0000074 nanA CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0002217 nanA CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0008474 nanA CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0003340 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0007877 nanA CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0003283 nanA CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0000974 nanA CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0005890 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0008808 nanA CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0008556 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0080153 nanA CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0003699 nanA CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0004318 nanA CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0008887 nanA CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0009199 nanA CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0003726 nanA CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0008139 nanA CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0002279 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0007882 nanA CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0006397 nanA CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0007929 nanA CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0009734 nanA CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0009378 nanA CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0009843 nanA CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0007187 nanA CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0006166 nanA CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0008525 nanA CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0009315 nanA CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0008647 nanA CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0006416 nanA CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0028253 nanA CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0009730 nanA CDS
Automated Process Approved Go view Escherichia coli HS ADR-0008101 nanA CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0003738 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0003011 nanA CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0003297 nanA CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0003869 nanA CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0003859 nanA CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0003657 nanA CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0003693 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0177850 pseudogene
Automated Process Approved Go view Escherichia coli SE11 AFS-0003569 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0003518 nanA CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0134162 nanA CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0006070 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0003664 nanA CDS
Person Uncurated Go view Hafnia alvei ATCC 13337 [T] AMD-0004308 nanA CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0001305 nanA CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0007697 nanA CDS
Automated Process Uncurated Go view Salmonella Arizonae CDC 346-86 BCD-0004485 CDS
Person Approved Go view Salmonella Choleraesuis SC-B67 ADB-0003828 nanA CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0007950 nanA CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0010359 nanA CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0009803 nanA CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0007111 nanA CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0007996 nanA CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0006915 nanA CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0008425 nanA CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0006382 nanA CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0007093 nanA CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0007959 nanA CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0005107 nanA CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0003240 CDS
Person Approved Go view Salmonella Paratyphi A ATCC 9150 ADA-0003206 nanA CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0004180 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0008120 nanA CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0009038 nanA CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0007603 nanA CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0007274 nanA CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0112445 nanA CDS
Person Approved Go view Salmonella Typhi Ty2 ABS-0084976 nanA CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0004036 nanA CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0100361 nanA CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0005889 nanA CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0005970 nanA CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0008589 nanA CDS
Person Approved Go view Shigella boydii 227 ADV-0003463 nanA CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0008967 nanA CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0007301 nanA CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0003865 nanA CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0074678 nanA CDS
Person Approved Go view Shigella flexneri 301 ABT-0089498 nanA CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0003458 nanA CDS
Person Approved Go view Shigella sonnei 046 ADD-0003674 nanA CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0006233 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0002804 nanA CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB21073mutantTn5 InsertionRequest the StrainABE-0010579nanAnanA::Tn5KAN-I-SceI at position 414 in Plus orientation ,+ pKD460Blattner Laboratory
FB21074mutantTn5 InsertionRequest the StrainABE-0010579nanAnanA::Tn5KAN-I-SceI at position 414 in Plus orientation ,- pKD465Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 3367683 to 3378576
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0010567yhcGCDSforward33678273368954Downstream
ABE-0010569nanQCDScomplement33690143369478Downstream
ABE-0010571nanKCDScomplement33694753370350Downstream
ABE-0010575nanECDScomplement33703473371036Downstream
ABE-0010577nanTCDScomplement33710843372574Downstream
ABE-0010579nanACDScomplement33726833373576Matches
ABE-0010584nanRCDScomplement33736983374489Upstream
ABE-0010589dcuDCDSforward33748693376236Upstream
ABE-0010591sspBCDScomplement33762793376776Upstream
ABE-0010594sspACDScomplement33767823377420Upstream
ABE-0010597rpsICDScomplement33778153378207Upstream
ABE-0010599rplMCDScomplement33782233378651Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.