Escherichia coli K-12 MG1655
Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0011718 (aldB) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0011718 showshow history
Names
aldB yiaX JW3561 ECK3577 b3588
Product
aldehyde dehydrogenase B
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 3754973 3756511 1539 513
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR012303 (IPR012303 (DEPRECATED) NAD-dependent aldehyde dehydrogenase) Apr 2008
Automated Process Approved IPR015590 (IPR015590 Aldehyde dehydrogenase domain) Apr 2008
Automated Process Approved IPR016161 (IPR016161 Aldehyde/histidinol dehydrogenase) Jul 2018
Automated Process Approved PF00171 (PF00171 Aldehyde dehydrogenase family) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Uncurated GO biological process GO:0055114 (OBSOLETE) oxidation-reduction process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR015590, IPR016161
Reference: Escherichia coli K-12 MG1655
Jul 2018
Person Uncurated GO biological process GO:0016052 carbohydrate catabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO biological process GO:0008152 metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR015590, IPR016161
Reference: Escherichia coli K-12 MG1655
Jul 2018
Person Uncurated GO biological process GO:0009438 methylglyoxal metabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655
Feb 2008
Automated Process Uncurated GO molecular function GO:0016491 oxidoreductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR015590, IPR016161
Reference: Escherichia coli K-12 MG1655
Jul 2018
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name aldB Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655
Oct 1998
Person Approved synonym JW3561 Published Annotation - PubMed ID: 16397293 Jan 2006
Automated Process Approved synonym yiaX Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Person Approved synonym ECK3577 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b3588 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product aldehyde dehydrogenase B Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved alternate product name aldehyde dehydrogenase B (lactaldehyde dehydrogenase) Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655
Jan 2006
Protein Family
Automated Process Approved protein family IPR012303 (DEPRECATED) NAD-dependent aldehyde dehydrogenase Protein Sequence Similarity - InterPro Domain: IPR012303
Reference: Escherichia coli K-12 MG1655
Apr 2008
Automated Process Approved protein family PF00171 Aldehyde dehydrogenase family Protein Sequence Similarity - PFAM Model Name: PF00171
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Approved protein family IPR016161 Aldehyde/histidinol dehydrogenase Protein Sequence Similarity - InterPro Domain: IPR016161
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Approved protein family IPR015590 Aldehyde dehydrogenase domain Protein Sequence Similarity - InterPro Domain: IPR015590
Reference: Escherichia coli K-12 MG1655
Apr 2008
Other
Automated Process Uncurated EC number 1.-.-.- Published Annotation - Database Name: EC2GO: GO:0016491
Reference: Escherichia coli K-12 MG1655
Jul 2018
Person Approved EC number 1.2.1.- Published Annotation - Swiss-Prot Accession Number: P37685 Feb 2006
Person Uncurated MultiFun 1.1.1 (metabolism; carbon utilization; carbon compounds) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 1.7.23 (metabolism; central intermediary metabolism; methylglyoxal metabolism) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref EcoGene:EG12292 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Person Approved db xref UniProtKB:P37685 Published Annotation - Swiss-Prot Accession Number: P37685 Jan 2005
Automated Process Approved db xref GeneID:948104 Published Annotation - Entrez Gene Database ID: 948104
Reference: Escherichia coli K-12 MG1655
Jun 2009
Automated Process Approved db xref GI:87082295 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P37685 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved db xref ASAP:ABE-0011718 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Person Approved function enzyme; Degradation of small molecules: Carbon compounds Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655
Oct 1998
Automated Process Approved note aldehyde dehydrogenase B (lactaldehyde dehydrogenase); GO_process: GO:0016052 - carbohydrate catabolic process; GO_process: GO:0009438 - methylglyoxal metabolic process Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Approved protein id AAC76612.2 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Predicted by PSORTb - PSORTb Final_Score: 9.26
Reference: Escherichia coli K-12 MG1655
Feb 2008
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655
Mar 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655
Jan 2009
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655
Feb 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655
Oct 2013
Gene Ontology
Automated Process Uncurated GO biological process GO:0008152 metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR015590, IPR016161
Reference: Escherichia coli K-12 MG1655
Jul 2018
Automated Process Uncurated GO biological process GO:0055114 (OBSOLETE) oxidation-reduction process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR015590, IPR016161
Reference: Escherichia coli K-12 MG1655
Jul 2018
Person Uncurated GO biological process GO:0009438 methylglyoxal metabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Person Uncurated GO biological process GO:0016052 carbohydrate catabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655
Feb 2008
Automated Process Uncurated GO molecular function GO:0016491 oxidoreductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR015590, IPR016161
Reference: Escherichia coli K-12 MG1655
Jul 2018
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0001779 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002217 aldB CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002146 aldB CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0001142 aldB CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0001964 aldB CDS
Ortholog
Automated Process Uncurated Go view Brenneria salicis ATCC 15712 [T] AMK-0001538 CDS
Automated Process Uncurated Go view Brenneria sp. EniD312 AKG-0001068 CDS
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0004162 aldB CDS
Automated Process Uncurated Go view Cedecea davisae ATCC 33431 [T] ALZ-0004346 CDS
Automated Process Uncurated Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0000186 aldB CDS
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0000687 aldB CDS
Automated Process Uncurated Go view Dickeya chrysanthemi Ech1591 AFK-0004449 CDS
Person Uncurated Go view Dickeya dadantii 3937 ABF-0016109 aldB CDS
Automated Process Uncurated Go view Dickeya parazeae Ech586 AGC-0004182 CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0000444 CDS
Automated Process Uncurated Go view Erwinia amylovora ATCC 49946 ACW-0302508 CDS
Automated Process Uncurated Go view Erwinia amylovora ATCC BAA-2158 APN-0002292 aldB CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0001453 aldB CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0004714 aldB CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0007481 aldB CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0003646 aldB CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0000590 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0005458 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0006995 aldB CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0005263 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0080585 aldB CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0004098 aldB CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0005043 acoD CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0006922 aldB CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0005129 aldB CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0004081 aldB CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0008518 aldB CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0004909 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0008289 aldB CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0006804 aldB CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0006351 aldB CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0005074 aldB CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0009792 aldB CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0005157 aldB CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0007604 aldB CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0005389 aldB CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0005454 aldB CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0005296 aldB CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0005183 aldB CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0005782 aldB CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0028653 aldB CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0005363 aldB CDS
Automated Process Approved Go view Escherichia coli HS ADR-0008482 aldB CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0004273 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0003344 aldB CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0003777 aldB CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0004200 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0004245 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0178263 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0003937 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0003912 aldB CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0134537 aldB CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0002832 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0004138 aldB CDS
Automated Process Uncurated Go view Ewingella americana ATCC 33852 [T] AMA-0003606 CDS
Person Uncurated Go view Hafnia alvei ATCC 13337 [T] AMD-0000111 CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0003000 aldB CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0002571 CDS
Automated Process Uncurated Go view Musicola paradisiaca Ech703 AGA-0004442 CDS
Automated Process Uncurated Go view Pantoea agglomerans IG1 APM-0000895 CDS
Automated Process Uncurated Go view Pantoea agglomerans SL1-M5 ALY-0004193 CDS
Automated Process Uncurated Go view Pantoea sp. aB AJV-0003308 CDS
Automated Process Uncurated Go view Pantoea stewartii DC283 ACV-0287318 aldB CDS
Automated Process Uncurated Go view Pantoea vagans C9-1 ALK-0004782 aldB CDS
Person Uncurated Go view Pectobacterium atrosepticum SCRI1043 ABL-0060295 aldB CDS
Automated Process Uncurated Go view Pectobacterium brasiliensis 1692 AED-0000082 aldB CDS
Person Uncurated Go view Pectobacterium carotovorum WPP14 ADT-0001234 aldB CDS
Automated Process Uncurated Go view Pectobacterium parmentieri WPP163 AFJ-0000203 aldB CDS
Automated Process Uncurated Go view Pectobacterium sp. PC1 AFL-0009468 CDS
Person Uncurated Go view Pseudomonas syringae pv. tomato DC3000 ADG-0000741 CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0008061 aldB CDS
Automated Process Uncurated Go view Salmonella Arizonae CDC 346-86 BCD-0004161 CDS
Person Uncurated Go view Salmonella Choleraesuis SC-B67 ADB-0004217 aldB CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0008312 aldB CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0011505 aldB CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0011472 aldB CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0005184 aldB CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0008361 aldB CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0005710 aldB CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0006091 aldB CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0006911 aldB CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0007456 aldB CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0008313 aldB CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0006532 aldB CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0003568 CDS
Person Uncurated Go view Salmonella Paratyphi A ATCC 9150 ADA-0003530 aldB CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0004592 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0008485 aldB CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0004507 aldB CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0007947 aldB CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0004753 aldB CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0113635 aldB CDS
Person Uncurated Go view Salmonella Typhi Ty2 ABS-0085565 aldB CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0004444 aldB CDS
Person Uncurated Go view Salmonella Typhimurium LT2 [T] ABU-0101379 aldB CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0005531 aldB CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0004957 aldB CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0005279 aldB CDS
Automated Process Uncurated Go view Serratia marcescens subsp. marcescens ATCC 13880 [T] AMF-0003403 CDS
Person Uncurated Go view Shewanella oneidensis MR-1 ACY-0268132 aldA CDS
Person Approved Go view Shigella boydii 227 ADV-0003916 aldB CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0009424 aldB CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0003135 aldB CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0075422 aldB CDS
Person Approved Go view Shigella flexneri 301 ABT-0089848 aldB CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0004194 aldB CDS
Person Uncurated Go view Xenorhabdus nematophila ATCC 19061 [T] ADW-0001478 aldB CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis IP31758 ADS-0005833 CDS
Person Uncurated Go view Yersinia pseudotuberculosis IP32953 ACZ-0002281 CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis PB1/+ AFV-0002698 CDS
Automated Process Uncurated Go view Yersinia pseudotuberculosis YPIII AFW-0002335 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0004706 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0001132 CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)

Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB21320mutantTn5 InsertionRequest the StrainABE-0011718aldBaldB::Tn5KAN-I-SceI at position 603 in Plus orientation ,- pKD465Blattner Laboratory
FB21321mutantTn5 InsertionRequest the StrainABE-0011718aldBaldB::Tn5KAN-I-SceI at position 603 in Plus orientation ,+ pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 3749973 to 3761511
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0011700sgbUCDSforward37492323750092Downstream
ABE-0011702sgbECDSforward37500863750781Downstream
ABE-0285168ysaCpseudogenecomplement37508133750916Downstream
ABE-0285169ysaDpseudogeneforward37509173751094Downstream
ABE-0011704yiaTCDScomplement37511283751868Downstream
ABE-0011710yiaUCDSforward37519923752966Downstream
ABE-0011712yiaVCDScomplement37529633754099Downstream
ABE-0011714yiaWCDScomplement37541053754428Downstream
ABE-0285622repeat_regionforward37548603754941Downstream
ABE-0011718aldBCDScomplement37549733756511Matches
ABE-0011725yiaYCDScomplement37566763757827Upstream
ABE-0011729selBCDScomplement37580173759861Upstream
ABE-0011731selACDScomplement37598583761249Upstream
ABE-0011736yibFCDScomplement37613473761955Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.