Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0011849 (waaL) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0011849 showshow history
Names
waaL JW3597 ECK3612 rfaL b3622
Product
O-antigen ligase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 3796948 3798207 1260 420
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR007016 (IPR007016 O-antigen ligase-related) Apr 2008
Automated Process Approved PF04932 (PF04932 O-Antigen ligase) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Uncurated GO biological process GO:0009244 lipopolysaccharide core region biosynthetic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Person Uncurated GO molecular function GO:0042280 (OBSOLETE) cell surface antigen activity, host-interacting Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Automated Process Approved name waaL Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved synonym rfaL Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym ECK3612 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW3597 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b3622 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product O-antigen ligase Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved alternate product name O-antigen ligase; lipopolysaccharide core biosynthesis Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Person Uncurated alternate product name O-antigen ligase Published Annotation - Database Name: GenProtEC Jan 2006
Protein Family
Automated Process Approved protein family IPR007016 O-antigen ligase-related Protein Sequence Similarity - InterPro Domain: IPR007016
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF04932 O-Antigen ligase Protein Sequence Similarity - PFAM Model Name: PF04932
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Person Uncurated MultiFun 6.1 (cell structure; membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 6.3 (cell structure; surface antigens (ECA, O antigen of LPS)) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 7.3 (location of gene products; inner membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 1.6.3.2 (metabolism; macromolecules (cellular constituent) biosynthesis; lipopolysaccharide; core region) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P27243 Published Annotation - Swiss-Prot Accession Number: P27243 Jan 2005
Automated Process Approved db xref GeneID:948148 Published Annotation - Entrez Gene Database ID: 948148
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref GI:1790052 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P27243 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0011849 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG11424 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved function enzyme; Macromolecule metabolism: Lipopolysaccharide Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note O-antigen ligase; lipopolysaccharide core biosynthesis; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_function: GO:0042280 - cell surface antigen activity, host-interacting; GO_process: GO:0009244 - lipopolysaccharide core region biosynthetic process Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC76646.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Membrane Predicted by PSORTb - PSORTb Final_Score: 10.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Person Uncurated GO biological process GO:0009244 lipopolysaccharide core region biosynthetic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Person Uncurated GO molecular function GO:0042280 (OBSOLETE) cell surface antigen activity, host-interacting Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0003847 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0004457 rfaL CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0003872 rfaL CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0003418 waaL CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0004393 waaL CDS
Ortholog
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0003376 rfaL CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0003742 rfaL CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 3791948 to 3803207
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0011832kblCDScomplement37913553792551Upstream
ABE-0011838htrLCDScomplement37928263793683Upstream
ABE-0011843rfaDCDSforward37939873794919Upstream
ABE-0011845waaFCDSforward37949293795975Upstream
ABE-0011847waaCCDSforward37959793796938Upstream
ABE-0011849waaLCDSforward37969483798207Matches
ABE-0285797yibXCDScomplement37977723798014Contained Within
ABE-0285798yibYCDScomplement37980393798149Contained Within
ABE-0011851waaUCDScomplement37982393799312Downstream
ABE-0011854waaZCDScomplement37993453800196Downstream
ABE-0011856waaYCDScomplement38002673800965Downstream
ABE-0011858waaJCDScomplement38009833801999Downstream
ABE-0011860waaOCDScomplement38020393803058Downstream
ABE-0011862waaBCDScomplement38030583804137Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.