Escherichia coli K-12 MG1655
About ASAP Data Release Policy University of Wisconsin—Madison
Home    Overview    Annotations    BLAST    Downloads    Comparative Genomics    Experiments    Strains    Help (this script)    User: guest  / Log On   
Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0011866 (waaP) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0011866 showshow history
Names
waaP JW3605 ECK3620 rfaP b3630
Product
lipopolysaccharide core heptose (I) kinase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 3805153 3805950 798 266
Sequence

Get full DNA or protein sequence

View sequence with flanking regions: bases upstream and bases downstream

Context

Browse sequence in GaPP

You last hovered over feature (none)
Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR010440 (IPR010440 (DEPRECATED) Lipopolysaccharide kinase) Apr 2008
Automated Process Approved IPR011009 (IPR011009 Protein kinase-like domain superfamily) Apr 2008
Automated Process Approved PF06293 (PF06293 Lipopolysaccharide kinase (Kdo/WaaP) family) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Uncurated GO biological process GO:0009244 lipopolysaccharide core region biosynthetic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0009103 lipopolysaccharide biosynthetic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR010440
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR010440
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO molecular function GO:0016773 phosphotransferase activity, alcohol group as acceptor GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR010440
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO molecular function GO:0042280 (OBSOLETE) cell surface antigen activity, host-interacting Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO molecular function GO:0005524 ATP binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR010440
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Automated Process Approved name waaP Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved synonym JW3605 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym ECK3620 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym rfaP Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved locus tag b3630 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product lipopolysaccharide core heptose (I) kinase Published Annotation - EcoCyc Accession Number: EG11340
Reference: Escherichia coli K-12 MG1655 		Oct 2021
Person Approved alternate product name lipopolysaccharide core biosynthesis; phosphorylation of core heptose; attaches phosphate-containing substrate to LPS co Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Person Approved alternate product name kinase that phosphorylates core heptose of lipopolysaccharide Published Annotation - PubMed ID: 16397293 Jan 2006
Person Uncurated alternate product name lipopolysaccharide core biosynthesis; kinase for core heptose of lipopolysaccharide Published Annotation - Database Name: GenProtEC Jan 2006
Protein Family
Automated Process Approved protein family IPR011009 Protein kinase-like domain superfamily Protein Sequence Similarity - InterPro Domain: IPR011009
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR010440 (DEPRECATED) Lipopolysaccharide kinase Protein Sequence Similarity - InterPro Domain: IPR010440
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF06293 Lipopolysaccharide kinase (Kdo/WaaP) family Protein Sequence Similarity - PFAM Model Name: PF06293
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Other
Person Uncurated EC number 2.7.1.- Protein Sequence Similarity - InterPro Domain: IPR010440
Reference: Escherichia coli K-12 MG1655 		Dec 2008
Person Uncurated MultiFun 6.3 (cell structure; surface antigens (ECA, O antigen of LPS)) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 1.6.3.2 (metabolism; macromolecules (cellular constituent) biosynthesis; lipopolysaccharide; core region) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 7.3 (location of gene products; inner membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG11340 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0011866 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P25741 Published Annotation - Swiss-Prot Accession Number: P25741 Jan 2005
Automated Process Approved db xref GeneID:948150 Published Annotation - Entrez Gene Database ID: 948150
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref GI:1790060 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P25741 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved function enzyme; Macromolecule metabolism: Lipopolysaccharide Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note lipopolysaccharide core biosynthesis; phosphorylation of core heptose; attaches phosphate-containing substrate to LPS co; GO_component: GO:0019866 - organelle inner membrane; GO_function: GO:0042280 - cell surface antigen activity, host-interacting; GO_process: GO:0009244 - lipopolysaccharide core region biosynthetic process Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC76654.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Predicted by PSORTb - PSORTb Final_Score: 8.96
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Person Uncurated GO biological process GO:0009244 lipopolysaccharide core region biosynthetic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0009103 lipopolysaccharide biosynthetic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR010440
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR010440
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0005524 ATP binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR010440
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0016773 phosphotransferase activity, alcohol group as acceptor GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR010440
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO molecular function GO:0042280 (OBSOLETE) cell surface antigen activity, host-interacting Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0004240 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0003006 rfaP CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0003187 rfaP CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0010424 inaA CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0002912 waaP CDS
Ortholog
Automated Process Uncurated Go view Brenneria salicis ATCC 15712 [T] AMK-0001489 CDS
Automated Process Uncurated Go view Brenneria sp. EniD312 AKG-0002473 CDS
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0004454 rfaP CDS
Automated Process Uncurated Go view Cedecea davisae ATCC 33431 [T] ALZ-0004110 CDS
Automated Process Uncurated Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0000147 rfaP CDS
Automated Process Uncurated Go view Citrobacter freundii ATCC 8090 [T] AVI-0000650 rfaP CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0001540 CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0004670 rfaP CDS
Automated Process Uncurated Go view Escherichia coli 536 (UPEC) AEF-0003767 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0007439 rfaP CDS
Automated Process Uncurated Go view Escherichia coli APEC O1 (APEC) AEG-0003684 rfaP CDS
Automated Process Uncurated Go view Escherichia coli ATCC 8739 AEM-0005410 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0010696 rfaP CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0008105 rfaP pseudogene
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0080631 rfaP CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0008926 rfaP CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0007441 rfaP CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0004122 rfaP CDS
Automated Process Uncurated Go view Escherichia coli E24377A (ETEC) ADO-0008563 rfaP CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0005554 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0008331 rfaP CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0006845 rfaP CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0006396 rfaP CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0006860 rfaP CDS
Automated Process Uncurated Go view Escherichia coli EC4115 (EHEC) BAS-0009834 rfaP CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0005113 rfaP CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0007647 rfaP CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0005432 rfaP CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0005498 rfaP CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0005340 rfaP CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0005226 rfaP CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0005825 rfaP CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0028699 waaP CDS
Automated Process Uncurated Go view Escherichia coli F11 (UPEC) ADQ-0005323 rfaP_2 CDS
Automated Process Uncurated Go view Escherichia coli HS ADR-0008527 rfaP CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0003384 rfaP CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0003734 rfaP CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0004236 CDS
Automated Process Uncurated Go view Escherichia coli SE11 AFS-0003982 CDS
Automated Process Uncurated Go view Escherichia coli SMS-3-5 AFT-0003962 rfaP CDS
Person Uncurated Go view Escherichia coli Sakai (EHEC) ACA-0134579 CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0002880 CDS
Automated Process Uncurated Go view Escherichia coli UTI89 (UPEC) AEA-0004182 rfaP CDS
Person Uncurated Go view Hafnia alvei ATCC 13337 [T] AMD-0000835 CDS
Person Uncurated Go view Pseudomonas syringae pv. tomato DC3000 ADG-0005078 waaP CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0008105 CDS
Automated Process Uncurated Go view Salmonella Arizonae CDC 346-86 BCD-0004111 CDS
Person Uncurated Go view Salmonella Choleraesuis SC-B67 ADB-0004259 rfaP CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0008354 CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0011659 rfaP CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0011326 rfaP CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0005141 CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0008404 CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0005753 CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0006050 CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0006956 CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0007501 CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0008356 CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0006490 CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0003609 CDS
Person Uncurated Go view Salmonella Paratyphi A ATCC 9150 ADA-0003572 waaP CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0004637 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0008526 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0004465 CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0007988 CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0004795 CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0113542 waaP CDS
Person Uncurated Go view Salmonella Typhi Ty2 ABS-0085523 waaP CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0004488 rfaP CDS
Person Uncurated Go view Salmonella Typhimurium LT2 [T] ABU-0101505 rfaP CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0005487 CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0004915 CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0008402 CDS
Person Uncurated Go view Shigella boydii 227 ADV-0003963 rfaP CDS
Automated Process Uncurated Go view Shigella boydii BS512 ADJ-0009475 rfaP CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0003207 rfaP CDS
Person Uncurated Go view Shigella dysenteriae 197 ADU-0004576 waaP CDS
Person Uncurated Go view Shigella flexneri 2457T ABQ-0075376 waaP CDS
Person Uncurated Go view Shigella flexneri 301 ABT-0089889 waaP CDS
Automated Process Uncurated Go view Shigella flexneri 8401 BAG-0004147 waaP CDS
Person Uncurated Go view Shigella sonnei 046 ADD-0004117 waaP CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 3800153 to 3810950
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0011854waaZCDScomplement37993453800196Downstream
ABE-0011856waaYCDScomplement38002673800965Downstream
ABE-0011858waaJCDScomplement38009833801999Downstream
ABE-0011860waaOCDScomplement38020393803058Downstream
ABE-0011862waaBCDScomplement38030583804137Downstream
ABE-0011864waaSCDScomplement38041813805116Downstream
ABE-0011866waaPCDScomplement38051533805950Matches
ABE-0011868waaGCDScomplement38059433807067Overlaps Upstream
ABE-0011870waaQCDScomplement38070643808098Upstream
ABE-0285791rirAncRNAcomplement38081663808238Upstream
ABE-0011873waaACDSforward38085403809817Upstream
ABE-0011875coaDCDSforward38098253810304Upstream
ABE-0285626repeat_regionforward38103123810333Upstream
ABE-0011877mutMCDScomplement38103433811152Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.