Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0012912 (gldA) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0012912 showshow history
Names
gldA JW5556 ECK3937 b3945
Product
L-1,2-propanediol dehydrogenase/glycerol dehydrogenase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 4137932 4139035 1104 368
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR001670 (IPR001670 Alcohol dehydrogenase, iron-type/glycerol dehydrogenase gldA) Apr 2008
Automated Process Approved IPR016205 (IPR016205 Glycerol dehydrogenase) Jul 2018
Automated Process Approved PF00465 (PF00465 Iron-containing alcohol dehydrogenase) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Uncurated GO biological process GO:0055114 (OBSOLETE) oxidation-reduction process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR001670, IPR016205
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO biological process GO:0008152 metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001670
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO biological process GO:0006071 glycerol metabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Uncurated GO molecular function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR016205
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0046872 metal ion binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001670
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0016491 oxidoreductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001670
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name gldA Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym ECK3937 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW5556 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b3945 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product L-1,2-propanediol dehydrogenase/glycerol dehydrogenase Published Annotation - EcoCyc Accession Number: EG11904
Reference: Escherichia coli K-12 MG1655 		Sep 2021
Person Approved alternate product name glycerol dehydrogenase, (NAD) Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Person Uncurated alternate product name NADPH-dependent aldehyde reductase Experimental - PubMed ID: 25108218
Reference: Escherichia coli K-12 BW25113 		Sep 2021
Person Uncurated alternate product name glycerol dehydrogenase, NAD Published Annotation - Database Name: GenProtEC Jan 2006
Protein Family
Automated Process Approved protein family PF00465 Iron-containing alcohol dehydrogenase Protein Sequence Similarity - PFAM Model Name: PF00465
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR016205 Glycerol dehydrogenase Protein Sequence Similarity - InterPro Domain: IPR016205
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR001670 Alcohol dehydrogenase, iron-type/glycerol dehydrogenase gldA Protein Sequence Similarity - InterPro Domain: IPR001670
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Automated Process Uncurated EC number 1.1.-.- Published Annotation - Database Name: EC2GO: GO:0016614
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated EC number 1.-.-.- Published Annotation - Database Name: EC2GO: GO:0016491
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Person Approved EC number 1.1.1.6 Published Annotation - Swiss-Prot Accession Number: P0A9S5 Feb 2006
Person Uncurated MultiFun 1.7.6 (metabolism; central intermediary metabolism; glycerol metabolism) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:87082352 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P0A9S5 Published Annotation - Swiss-Prot Accession Number: P0A9S5 Jan 2006
Automated Process Approved db xref GeneID:948440 Published Annotation - Entrez Gene Database ID: 948440
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Automated Process Approved db xref EcoGene:EG11904 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0012912 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P0A9S5 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved function enzyme; Central intermediary metabolism: Pool, multipurpose conversions Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note glycerol dehydrogenase, (NAD); GO_process: GO:0006071 - glycerol metabolic process Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC76927.2 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Predicted by PSORTb - PSORTb Final_Score: 9.26
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0008152 metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001670
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO biological process GO:0006071 glycerol metabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO biological process GO:0055114 (OBSOLETE) oxidation-reduction process GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR001670, IPR016205
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Uncurated GO molecular function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR016205
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0046872 metal ion binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001670
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0016491 oxidoreductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001670
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Homolog
Person Approved Go view Pectobacterium atrosepticum SCRI1043 ABL-0061082 gldA CDS
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0003894 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002089 gldA CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002077 gldA CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0003140 gldA CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0001807 gldA CDS
Ortholog
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0004505 gldA CDS
Automated Process Approved Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0004309 gldA CDS
Automated Process Approved Go view Citrobacter freundii ATCC 8090 [T] AVI-0000325 gldA_1 CDS
Automated Process Uncurated Go view Enterobacter cloacae subsp. cloacae ATCC 13047 [T] (AToL DRAFT) AMB-0000146 CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0001885 gldA CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0007517 gldA CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0004204 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0007043 gldA CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0004009 gldA CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0000223 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0010266 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0009133 gldA CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0009428 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0081102 gldA CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0004576 gldA CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0005130 dhaD CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0006424 gldA CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0007603 gldA CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0004532 gldA CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0008919 gldA CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0001192 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0008736 gldA CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0007249 gldA CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0007440 gldA CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0006888 gldA CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0010238 gldA CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0007848 gldA CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0008053 gldA CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0007598 gldA CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0007679 gldA CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0007254 gldA CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0007204 gldA CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0007121 gldA CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0029106 gldA CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0007816 gldA CDS
Automated Process Approved Go view Escherichia coli HS ADR-0008865 gldA CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0004506 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0003655 gldA CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0003409 gldA CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0004661 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0004636 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0004393 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0004553 gldA CDS
Person Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0179620 gldA CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0004326 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0004388 gldA CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0134972 gldA CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0001786 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0004545 gldA CDS
Automated Process Uncurated Go view Ewingella americana ATCC 33852 [T] AMA-0001220 CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0004289 gldA CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0002302 CDS
Automated Process Uncurated Go view Pantoea ananatis AJ13355 AOV-0000244 dhaD CDS
Automated Process Uncurated Go view Pantoea ananatis B1-9 APL-0003992 CDS
Automated Process Uncurated Go view Pantoea ananatis LMG 20103 AKB-0000943 dhaD CDS
Automated Process Uncurated Go view Pantoea ananatis PA13 APK-0003372 CDS
Automated Process Uncurated Go view Pantoea stewartii DC283 ACV-0290646 gldA CDS
Automated Process Uncurated Go view Pectobacterium brasiliensis 1692 AED-0000439 gldA CDS
Automated Process Uncurated Go view Pectobacterium carotovorum WPP14 ADT-0000396 CDS
Automated Process Uncurated Go view Pectobacterium parmentieri WPP163 AFJ-0001794 CDS
Automated Process Uncurated Go view Pectobacterium sp. PC1 AFL-0005324 CDS
Person Uncurated Go view Photorhabdus luminescens subsp. laumondii TTO1 [T] ACD-0146186 gldA CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0008520 CDS
Automated Process Uncurated Go view Salmonella Arizonae CDC 346-86 BCD-0003721 CDS
Person Uncurated Go view Salmonella Choleraesuis SC-B67 ADB-0004695 gldA CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0008755 CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0012891 gldA CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0010060 gldA CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0007436 CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0008799 CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0007774 CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0007710 CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0007409 CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0007911 CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0008789 CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0008694 CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0003991 CDS
Person Uncurated Go view Salmonella Paratyphi A ATCC 9150 ADA-0003950 gldA CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0005112 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0008934 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0006645 CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0008389 CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0006981 CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0112938 gldA CDS
Person Uncurated Go view Salmonella Typhi Ty2 ABS-0085233 gldA CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0004945 gldA_2 CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0102664 gldA CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0005045 CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0007782 CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0005995 CDS
Automated Process Uncurated Go view Serratia marcescens subsp. marcescens ATCC 13880 [T] AMF-0001613 CDS
Person Approved Go view Shigella boydii 227 ADV-0004321 gldA CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0009848 gldA CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0004272 gldA CDS
Person Uncurated Go view Shigella flexneri 2457T ABQ-0074954 gldA pseudogene
Person Uncurated Go view Shigella flexneri 301 ABT-0256157 gldA pseudogene
Person Approved Go view Shigella sonnei 046 ADD-0004483 gldA CDS
Person Uncurated Go view Xenorhabdus nematophila ATCC 19061 [T] ADW-0001083 gldA CDS
Automated Process Uncurated Go view Yersinia bercovieri ATCC 43970 [T] AEK-0000018 CDS
Automated Process Uncurated Go view Yersinia enterocolitica 8081 BAH-0001635 gldA CDS
Automated Process Uncurated Go view Yersinia frederiksenii ATCC 33641 [T] AEI-0003262 gldA_2 CDS
Automated Process Uncurated Go view Yersinia intermedia ATCC 29909 [T] AEH-0002189 gldA_1 CDS
Automated Process Uncurated Go view Yersinia mollaretii ATCC 43969 [T] AEJ-0003055 CDS
Molecular Interactions to toptop
Author Approval Feature Name Technique Evidence Comment Annotated
Person Approved ABE-0012912 gldA lambda repressor assay Doodle - bnumber: b3945 Oct 2003
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB21568mutantTn5 InsertionRequest the StrainABE-0012912gldAgldA::Tn5KAN-I-SceI at position 939 in Plus orientation ,+ pKD460Blattner Laboratory
FB21569mutantTn5 InsertionRequest the StrainABE-0012912gldAgldA::Tn5KAN-I-SceI at position 939 in Plus orientation ,- pKD465Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 4132932 to 4144035
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0012897metFCDSforward41326164133506Downstream
ABE-0012901katGCDSforward41338354136015Downstream
ABE-0012906yijECDSforward41361084137013Downstream
ABE-0012908yijFCDScomplement41370404137657Downstream
ABE-0285660repeat_regionforward41377894137886Downstream
ABE-0012912gldACDScomplement41379324139035Matches
ABE-0012915fsaBCDScomplement41390464139708Upstream
ABE-0012920ptsACDScomplement41397204142221Upstream
ABE-0012928frwCCDSforward41425304143609Upstream
ABE-0012930frwBCDSforward41436244143944Upstream
ABE-0012934pflDCDSforward41439954146292Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.