Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0012934 (pflD) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0012934 showshow history
Names
pflD yijL JW3923 ECK3942 b3951
Product
putative formate acetyltransferase 2
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 4143995 4146292 2298 766
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR004184 (IPR004184 Pyruvate formate lyase domain) Apr 2008
Automated Process Approved IPR010098 (IPR010098 Glycyl radical enzyme, PFL2/glycerol dehydratase family) Apr 2008
Automated Process Approved IPR001150 (IPR001150 Glycine radical domain) Apr 2008
Automated Process Approved PF01228 (PF01228 Glycine radical) Jul 2018
Automated Process Approved PF02901 (PF02901 Pyruvate formate lyase-like) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Uncurated GO biological process GO:0009061 anaerobic respiration Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0008152 metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001150
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0006006 glucose metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004184
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004184
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0003824 catalytic activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001150
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0008861 formate C-acetyltransferase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004184
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name pflD Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym JW3923 Published Annotation - PubMed ID: 16397293 Jan 2006
Automated Process Approved synonym yijL Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved synonym ECK3942 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b3951 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Uncurated product putative formate acetyltransferase 2 Published Annotation - EcoCyc Accession Number: EG11910
Reference: Escherichia coli K-12 MG1655 		Oct 2021
Person Uncurated alternate product name putative pyruvate formate lyase II Published Annotation - Database Name: GenProtEC Jan 2006
Person Approved alternate product name predicted formate acetyltransferase 2 (pyruvate formate lyase II) Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved alternate product name formate acetyltransferase 2 Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Protein Family
Automated Process Approved protein family IPR010098 Glycyl radical enzyme, PFL2/glycerol dehydratase family Protein Sequence Similarity - InterPro Domain: IPR010098
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF02901 Pyruvate formate lyase-like Protein Sequence Similarity - PFAM Model Name: PF02901
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF01228 Glycine radical Protein Sequence Similarity - PFAM Model Name: PF01228
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR004184 Pyruvate formate lyase domain Protein Sequence Similarity - InterPro Domain: IPR004184
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR001150 Glycine radical domain Protein Sequence Similarity - InterPro Domain: IPR001150
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Person Approved EC number 2.3.1.54 Published Annotation - Swiss-Prot Accession Number: P32674 Feb 2006
Person Uncurated MultiFun 1.3.7 (metabolism; energy metabolism, carbon; anaerobic respiration) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG11910 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:1790388 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P32674 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved db xref UniProtKB:P32674 Published Annotation - Swiss-Prot Accession Number: P32674 Jan 2005
Automated Process Approved db xref ASAP:ABE-0012934 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:948454 Published Annotation - Entrez Gene Database ID: 948454
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Person Approved function enzyme; Energy metabolism, carbon: Anaerobic respiration Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note formate acetyltransferase 2; GO_process: GO:0009061 - anaerobic respiration Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC76933.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Predicted by PSORTb - PSORTb Final_Score: 9.97
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0008152 metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001150
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0006006 glucose metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004184
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO biological process GO:0009061 anaerobic respiration Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004184
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0003824 catalytic activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001150
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0008861 formate C-acetyltransferase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR004184
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0002559 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002858 pflD CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002811 pflD CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0002792 ybiW CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0002481 pflD CDS
Ortholog
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0004510 CDS
Automated Process Approved Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0004304 pflD CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0001890 CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0007512 CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0004209 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0007038 CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0004014 pflD CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0000218 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0010260 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0009128 CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0009433 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0081108 pflD CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0004581 CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0005135 CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0006429 CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0007598 CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0004537 pflD CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0008924 CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0001197 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0008741 CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0007254 CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0007445 CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0006883 CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0010243 CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0007843 CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0008058 CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0007603 CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0007684 CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0007248 CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0007198 CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0007126 CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0029113 pflD CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0007821 cutC_3 CDS
Automated Process Approved Go view Escherichia coli HS ADR-0008870 CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0004501 pflD CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0003660 pflD CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0003404 pflD CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0004656 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0004641 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0004388 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0004559 pflD CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0178678 pflD CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0004331 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0004393 CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0134979 pflD CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0001792 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0004551 pflD CDS
Person Uncurated Go view Hafnia alvei ATCC 13337 [T] AMD-0000980 CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0002297 CDS
Automated Process Uncurated Go view Pectobacterium parmentieri WPP163 AFJ-0002485 CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0008760 CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0012916 pflD CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0010040 pflD CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0007431 CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0007715 CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0008794 CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0008699 CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0005118 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0008939 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0006640 CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0004952 pflD CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0102681 pflD CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0006000 CDS
Person Approved Go view Shigella boydii 227 ADV-0004326 pflD CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0009853 CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0005773 CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0004278 pflD CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0074947 pflD CDS
Person Approved Go view Shigella flexneri 301 ABT-0090241 pflD CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0004273 pflD CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 4138995 to 4151292
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0012912gldACDScomplement41379324139035Upstream
ABE-0012915fsaBCDScomplement41390464139708Upstream
ABE-0012920ptsACDScomplement41397204142221Upstream
ABE-0012928frwCCDSforward41425304143609Upstream
ABE-0012930frwBCDSforward41436244143944Upstream
ABE-0012934pflDCDSforward41439954146292Matches
ABE-0012937pflCCDSforward41462584147136Overlaps Downstream
ABE-0012941frwDCDSforward41471384147479Downstream
ABE-0012943yijOCDScomplement41474664148317Downstream
ABE-0285661repeat_regionforward41484304148511Downstream
ABE-0012947eptCCDScomplement41485324150265Downstream
ABE-0012950ppcCDScomplement41504474153098Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.