Escherichia coli K-12 MG1655
About ASAP Data Release Policy University of Wisconsin—Madison
Home    Overview    Annotations    BLAST    Downloads    Comparative Genomics    Experiments    Strains    Help (this script)    User: guest  / Log On   
Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0012937 (pflC) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0012937 showshow history
Names
pflC yijM JW3924 ECK3943 b3952
Product
pyruvate formate lyase II activase
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 4146258 4147136 879 293
Sequence

Get full DNA or protein sequence

View sequence with flanking regions: bases upstream and bases downstream

Context

Browse sequence in GaPP

You last hovered over feature (none)
Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR011352 (IPR011352 (DEPRECATED) Pyruvate formate-lyase activating enzyme) Apr 2008
Automated Process Approved IPR007197 (IPR007197 Radical SAM) Apr 2008
Automated Process Approved IPR012839 (IPR012839 Organic radical enzyme activase) Apr 2008
Automated Process Approved IPR006638 (IPR006638 Elp3/MiaB/NifB) Apr 2008
Automated Process Approved IPR001989 (IPR001989 Radical-activating enzyme, conserved site) Apr 2008
Automated Process Approved PF04055 (PF04055 Radical SAM superfamily) Jul 2018
Automated Process Approved PF13353 (PF13353 4Fe-4S single cluster domain) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Uncurated GO biological process GO:0009061 anaerobic respiration Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0006800 (OBSOLETE) oxygen and reactive oxygen species metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001989
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0006006 glucose metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR011352
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO biological process GO:0006464 cellular protein modification process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR011352
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0051539 4 iron, 4 sulfur cluster binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001989
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0016491 oxidoreductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001989
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0051536 iron-sulfur cluster binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR007197
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0003824 catalytic activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR007197
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name pflC Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym ECK3943 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW3924 Published Annotation - PubMed ID: 16397293 Jan 2006
Automated Process Approved synonym yijM Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved locus tag b3952 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product pyruvate formate lyase II activase Published Annotation - PubMed ID: 16397293 Jan 2006
Person Uncurated alternate product name putative pyruvate formate lyase activating enzyme 2 Published Annotation - Database Name: GenProtEC Jan 2006
Person Approved alternate product name probable pyruvate formate lyase activating enzyme 2 Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Protein Family
Automated Process Approved protein family IPR011352 (DEPRECATED) Pyruvate formate-lyase activating enzyme Protein Sequence Similarity - InterPro Domain: IPR011352
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF04055 Radical SAM superfamily Protein Sequence Similarity - PFAM Model Name: PF04055
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF13353 4Fe-4S single cluster domain Protein Sequence Similarity - PFAM Model Name: PF13353
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR006638 Elp3/MiaB/NifB Protein Sequence Similarity - InterPro Domain: IPR006638
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR001989 Radical-activating enzyme, conserved site Protein Sequence Similarity - InterPro Domain: IPR001989
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR007197 Radical SAM Protein Sequence Similarity - InterPro Domain: IPR007197
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR012839 Organic radical enzyme activase Protein Sequence Similarity - InterPro Domain: IPR012839
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Person Approved EC number 1.97.1.4 Published Annotation - Swiss-Prot Accession Number: P32675 Feb 2006
Person Uncurated MultiFun 1.3.7 (metabolism; energy metabolism, carbon; anaerobic respiration) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 2.3.3 (information transfer; protein related; posttranslational modification) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 3.1.3.3 (regulation; type of regulation; posttranscriptional; inhibition / activation of enzymes) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG11911 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:948453 Published Annotation - Entrez Gene Database ID: 948453
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Person Approved db xref UniProtKB:P32675 Published Annotation - Swiss-Prot Accession Number: P32675 Jan 2005
Automated Process Approved db xref UniProtKB/Swiss-Prot:P32675 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:1790389 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0012937 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved function putative enzyme; Energy metabolism, carbon: Anaerobic respiration Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note probable pyruvate formate lyase activating enzyme 2; GO_process: GO:0009061 - anaerobic respiration; GO_process: GO:0006464 - protein modification process Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC76934.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Predicted by PSORTb - PSORTb Final_Score: 9.97
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Automated Process Approved GO biological process GO:0006800 (OBSOLETE) oxygen and reactive oxygen species metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001989
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO biological process GO:0009061 anaerobic respiration Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Person Uncurated GO biological process GO:0006464 cellular protein modification process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0006006 glucose metabolic process GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR011352
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR011352
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005737 cytoplasm GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0051536 iron-sulfur cluster binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR007197
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0051539 4 iron, 4 sulfur cluster binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001989
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0016491 oxidoreductase activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001989
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0003824 catalytic activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR007197
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0002814 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002861 pflC CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0002864 pflC CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0006852 pflC CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0002564 pflC CDS
Ortholog
Automated Process Uncurated Go view Buttiauxella agrestis ATCC 33320 [T] APC-0004511 CDS
Automated Process Approved Go view Citrobacter amalonaticus ATCC 25405 [T] AUW-0004303 pflC CDS
Automated Process Uncurated Go view Escherichia albertii TW07627 AFX-0001891 CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0007511 CDS
Automated Process Approved Go view Escherichia coli 536 (UPEC) AEF-0004210 CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0007037 CDS
Automated Process Approved Go view Escherichia coli APEC O1 (APEC) AEG-0004015 pflC CDS
Automated Process Approved Go view Escherichia coli ATCC 11775 [T] AMC-0000217 CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0010259 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0009127 CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0009434 CDS
Person Approved Go view Escherichia coli CFT073 (UPEC) ABR-0081109 pflC CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0004582 CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0005136 CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0006430 CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0007597 CDS
Automated Process Approved Go view Escherichia coli E2348/69 (EPEC) BCI-0004538 pflC CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0008925 CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0001198 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0008742 CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0007255 CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0007446 CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0006882 CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0010244 CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0007842 CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0008059 CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0007604 CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0007685 CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0007247 CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0007197 CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0007127 CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0029114 pflC CDS
Automated Process Approved Go view Escherichia coli F11 (UPEC) ADQ-0007822 cutD_2 CDS
Automated Process Approved Go view Escherichia coli HS ADR-0008871 CDS
Automated Process Uncurated Go view Escherichia coli J96 (UPEC) CQK-0004500 CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0003661 pflC CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0003403 pflC CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0004655 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0004642 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0004387 CDS
Automated Process Uncurated Go view Escherichia coli Nissle 1917 AML-0004560 CDS
Automated Process Uncurated Go view Escherichia coli RS218 (NMEC) ACK-0178679 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0004332 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0004394 CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0134980 pflC CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0001793 CDS
Automated Process Approved Go view Escherichia coli UTI89 (UPEC) AEA-0004552 pflC CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0002296 CDS
Automated Process Uncurated Go view Pectobacterium atrosepticum SCRI1043 ABL-0064086 CDS
Automated Process Uncurated Go view Pectobacterium parmentieri WPP163 AFJ-0003162 CDS
Person Uncurated Go view Salmonella Choleraesuis SC-B67 ADB-0004701 pflC CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0008761 CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0012920 pflC CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0010037 pflC CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0007430 CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0007716 CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0008795 CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0008700 CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0005119 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0008940 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0006639 CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0004953 pflC CDS
Person Approved Go view Salmonella Typhimurium LT2 [T] ABU-0102684 pflC CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0006001 CDS
Person Approved Go view Shigella boydii 227 ADV-0004327 pflC CDS
Automated Process Approved Go view Shigella boydii BS512 ADJ-0009854 CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0005772 CDS
Person Approved Go view Shigella dysenteriae 197 ADU-0004279 pflC CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0074946 pflC CDS
Person Approved Go view Shigella flexneri 301 ABT-0090242 pflC CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0004274 pflC CDS
Person Approved Go view Shigella sonnei 046 ADD-0004489 pflC CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB21572mutantTn5 InsertionRequest the StrainABE-0012937pflCpflC::Tn5KAN-I-SceI at position 367 in Minus orientation ,- pKD465Blattner Laboratory
FB21573mutantTn5 InsertionRequest the StrainABE-0012937pflCpflC::Tn5KAN-I-SceI at position 367 in Minus orientation ,+ pKD460Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 4141258 to 4152136
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0012920ptsACDScomplement41397204142221Upstream
ABE-0012928frwCCDSforward41425304143609Upstream
ABE-0012930frwBCDSforward41436244143944Upstream
ABE-0012934pflDCDSforward41439954146292Overlaps Upstream
ABE-0012937pflCCDSforward41462584147136Matches
ABE-0012941frwDCDSforward41471384147479Downstream
ABE-0012943yijOCDScomplement41474664148317Downstream
ABE-0285661repeat_regionforward41484304148511Downstream
ABE-0012947eptCCDScomplement41485324150265Downstream
ABE-0012950ppcCDScomplement41504474153098Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.