Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0013439 (phnE_1) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0013439 showshow history
Names
phnE_1 JW4065 ECK4097 phnE b4104
Product
membrane component of an ABC superfamily phosphonate/organophosphate ester transporter
Type
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 4322661 4323230 570 190
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR005769 (IPR005769 ABC transporter, permease PhnE/PtxC) Apr 2008
Automated Process Approved IPR000515 (IPR000515 ABC transporter type 1, transmembrane domain MetI-like) Apr 2008
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Uncurated GO biological process GO:0006793 phosphorus metabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0006810 transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000515
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0015716 organic phosphonate transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR005769
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000515
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO cellular component GO:0005887 integral component of plasma membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR005769
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0005215 transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000515
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0015604 (DEPRECATED) organic phosphonate transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR005769
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name phnE_1 Unpublished Sequence Analysis - Author Name, Email; Comment: Guy Plunkett III, guy@genome.wisc.edu
Reference: Escherichia coli K-12 MG1655 		Mar 2006
Person Approved synonym ECK4097 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym phnE Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym JW4065 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b4104 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product membrane component of an ABC superfamily phosphonate/organophosphate ester transporter Published Annotation - PubMed ID: 16397293
Reference: Escherichia coli K-12 		Mar 2008
Person Approved alternate product name membrane channel protein component of Pn transporter Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Person Uncurated alternate product name phosphonate transport protein, cryptic (ABC superfamily, membrane) Published Annotation - Database Name: GenProtEC Jan 2006
Protein Family
Automated Process Approved protein family IPR000515 ABC transporter type 1, transmembrane domain MetI-like Protein Sequence Similarity - InterPro Domain: IPR000515
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR005769 ABC transporter, permease PhnE/PtxC Protein Sequence Similarity - InterPro Domain: IPR005769
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Person Uncurated MultiFun 4.3.A.1.m (transport; Primary Active Transporters; Pyrophosphate Bond (ATP, GTP, P2) Hydrolysis-driven Active Transporters; The ATP-binding Cassette (ABC) Superfamily + ABC-type Uptake Permeases; ABC superfamily, membrane component) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 7.3 (location of gene products; inner membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 6.1 (cell structure; membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 10 (cryptic genes) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 4.S.8 (transport; substrate; alkylphosphonate) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 1.8.1 (metabolism; metabolism of other compounds; phosphorous metabolism) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated comment The coordinates of phnE_1 have been changed from those reported in the GenBank entry (GBAN:U00096.2) to correspond to the start site predicted in Escherichia coli EDL933 (EHEC) (ASAP:ABH-0029280), removing 17 codons from the N-terminus. Unpublished Sequence Analysis - Author Name, Email; Comment: Eric Neeno-Eckwall, ecneenoeckwa@wisc.edu
Reference: Escherichia coli K-12 MG1655 		Oct 2008
Person Approved db xref UniProtKB:P16683 Published Annotation - Swiss-Prot Accession Number: P16683 Jan 2005
Person Approved function transport; Transport of small molecules: Anions Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Uncurated subcellular localization Cytoplasmic Membrane Predicted by PSORTb - PSORTb Final_Score: 9.46
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Gene Ontology
Automated Process Approved GO biological process GO:0006810 transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000515
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO biological process GO:0006793 phosphorus metabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0015716 organic phosphonate transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR005769
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000515
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO cellular component GO:0005887 integral component of plasma membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR005769
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0015604 (DEPRECATED) organic phosphonate transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR005769
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0005215 transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000515
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB21664mutantTn5 InsertionRequest the StrainABE-0013439phnE_1phnE::Tn5KAN-I-SceI at position 242 in Minus orientation ,+ pKD460Blattner Laboratory
FB21665mutantTn5 InsertionRequest the StrainABE-0013439phnE_1phnE::Tn5KAN-I-SceI at position 242 in Minus orientation ,- pKD465Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 4317661 to 4328230
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0013421phnLCDScomplement43172154317895Downstream
ABE-0285676repeat_regionforward43179174317999Downstream
ABE-0013424phnKCDScomplement43180064318764Downstream
ABE-0013426phnJCDScomplement43187614319606Downstream
ABE-0013428phnICDScomplement43195994320663Downstream
ABE-0013430phnHCDScomplement43206634321247Downstream
ABE-0013433phnGCDScomplement43212444321696Downstream
ABE-0013435phnFCDScomplement43216974322422Downstream
ABE-0013437phnE_2CDSpcomplement43224434322811Overlaps Downstream
ABE-0285115phnEpseudogenecomplement43224434323230Contains
ABE-0013439phnE_1CDSpcomplement43226614323230Matches
ABE-0285677repeat_regionforward43232464323332Upstream
ABE-0013442phnDCDScomplement43233364324352Upstream
ABE-0013444phnCCDScomplement43243774325165Upstream
ABE-0013447yjdNCDScomplement43252984325741Upstream
ABE-0285678repeat_regionforward43258024326384Upstream
ABE-0013451yjdMCDScomplement43263994326734Upstream
ABE-0013456rdcACDSforward43271354329363Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.