Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0013495 (melB) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0013495 showshow history
Names
melB JW4081 ECK4113 mel-4 b4120
Product
melibiose:sodium symporter
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome forward 4343369 4344790 1422 474

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Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR001927 (IPR001927 Sodium:galactoside symporter) Apr 2008
Automated Process Approved IPR036259 (IPR036259 MFS transporter superfamily) Jul 2018
Automated Process Approved PF13347 (PF13347 MFS/sugar transport protein) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Person Uncurated GO biological process GO:0016052 carbohydrate catabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0006814 sodium ion transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001927
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001927
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0005215 transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001927
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name melB Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym ECK4113 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW4081 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym mel-4 Published Sequence Analysis - PubMed ID: 9729611 Aug 2003
Person Approved locus tag b4120 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product melibiose:sodium symporter Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved alternate product name melibiose permease II Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Person Uncurated alternate product name melibiose permease II (GPH family) Published Annotation - Database Name: GenProtEC Jan 2006
Protein Family
Automated Process Approved protein family PF13347 MFS/sugar transport protein Protein Sequence Similarity - PFAM Model Name: PF13347
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR036259 MFS transporter superfamily Protein Sequence Similarity - InterPro Domain: IPR036259
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR001927 Sodium:galactoside symporter Protein Sequence Similarity - InterPro Domain: IPR001927
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Person Uncurated MultiFun 4.2.A.2 (transport; Electrochemical potential driven transporters; Porters (Uni-, Sym- and Antiporters); The Glycoside-Pentoside-Hexuronide (GPH):Cation Symporter Family) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 1.1.1 (metabolism; carbon utilization; carbon compounds) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 7.3 (location of gene products; inner membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 4.S.117 (transport; substrate; melibiose) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 6.1 (cell structure; membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:948635 Published Annotation - Entrez Gene Database ID: 948635
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Person Approved db xref UniProtKB:P02921 Published Annotation - Swiss-Prot Accession Number: P02921 Jan 2005
Automated Process Approved db xref EcoGene:EG10578 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0013495 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:345297180 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref UniProtKB/Swiss-Prot:P02921 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved function transport; Transport of small molecules: Carbohydrates, organic acids, alcohols Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note melibiose permease II; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0019866 - organelle inner membrane; GO_process: GO:0016052 - carbohydrate catabolic process Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC77081.2 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Cytoplasmic Membrane Predicted by PSORTb - PSORTb Final_Score: 10.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iMC1010 Experimental - PubMed ID: 15129285
Reference: Escherichia coli K-12 MG1655 		Mar 2009
Person Uncurated subsystem component of in silico model iJR904 Experimental - PubMed ID: 12952533
Reference: Escherichia coli K-12 MG1655 		Feb 2009
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Person Uncurated GO biological process GO:0016052 carbohydrate catabolic process Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0006814 sodium ion transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001927
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO cellular component GO:0019866 organelle inner membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001927
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0005886 plasma membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Automated Process Approved GO molecular function GO:0005215 transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR001927
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0005125 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0002788 melB CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0003073 melB CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0005376 melB CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0003425 melB CDS
Ortholog
Automated Process Uncurated Go view Citrobacter freundii ATCC 8090 [T] AVI-0000132 melB_1 CDS
Person Uncurated Go view Citrobacter freundii ATCC 8090 [T] (AToL DRAFT) API-0005093 melB pseudogene
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0006406 melB CDS
Automated Process Uncurated Go view Escherichia coli 53638 (EIEC) ADP-0006846 melB CDS
Automated Process Approved Go view Escherichia coli ATCC 8739 AEM-0010056 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0006336 melB CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0005744 CDS
Automated Process Uncurated Go view Escherichia coli DEC7E (UM-IGS draft) CFX-0004790 melB CDS
Automated Process Uncurated Go view Escherichia coli DEC9A (UM-IGS draft) CFY-0005386 CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0006208 melB CDS
Automated Process Uncurated Go view Escherichia coli E22 (EPEC) ADL-0006670 melB CDS
Automated Process Approved Go view Escherichia coli E24377A (ETEC) ADO-0009111 melB CDS
Automated Process Uncurated Go view Escherichia coli EC4024 (EHEC) BBI-0003182 CDS
Automated Process Uncurated Go view Escherichia coli EC4042 (EHEC) BBA-0008960 melB CDS
Automated Process Uncurated Go view Escherichia coli EC4045 (EHEC) BBB-0007474 melB CDS
Automated Process Uncurated Go view Escherichia coli EC4076 (EHEC) BAZ-0007119 melB CDS
Automated Process Uncurated Go view Escherichia coli EC4113 (EHEC) BAY-0006205 melB CDS
Automated Process Approved Go view Escherichia coli EC4115 (EHEC) BAS-0010464 melB CDS
Automated Process Uncurated Go view Escherichia coli EC4196 (EHEC) BAX-0007134 melB CDS
Automated Process Uncurated Go view Escherichia coli EC4206 (EHEC) BAW-0008278 melB CDS
Automated Process Uncurated Go view Escherichia coli EC4401 (EHEC) BAV-0007251 melB CDS
Automated Process Uncurated Go view Escherichia coli EC4486 (EHEC) BAU-0005829 melB CDS
Automated Process Uncurated Go view Escherichia coli EC4501 (EHEC) BAT-0006577 melB CDS
Automated Process Uncurated Go view Escherichia coli EC508 (EHEC) BBJ-0005955 melB CDS
Automated Process Uncurated Go view Escherichia coli EC869 (EHEC) BBC-0005099 melB CDS
Person Approved Go view Escherichia coli EDL933 (EHEC) ABH-0029296 melB CDS
Automated Process Approved Go view Escherichia coli HS ADR-0009049 melB CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0003810 melB CDS
Person Approved Go view Escherichia coli K-12 W3110 ADY-0004209 melB CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR68 CGA-0004736 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR71 CGB-0004839 CDS
Automated Process Uncurated Go view Escherichia coli MOD1-ECOR8 CFZ-0004547 CDS
Automated Process Approved Go view Escherichia coli SE11 AFS-0004518 CDS
Automated Process Approved Go view Escherichia coli SMS-3-5 AFT-0004578 melB CDS
Person Approved Go view Escherichia coli Sakai (EHEC) ACA-0135214 melB CDS
Automated Process Uncurated Go view Escherichia coli TW14588 (EHEC) BBK-0003375 CDS
Person Uncurated Go view Klebsiella pneumoniae subsp. pneumoniae MGH 78578 AEP-0004556 melB CDS
Automated Process Uncurated Go view Pantoea ananatis AJ13355 AOV-0002514 melB CDS
Automated Process Uncurated Go view Pantoea ananatis B1-9 APL-0001488 CDS
Automated Process Uncurated Go view Pantoea ananatis LMG 20103 AKB-0003329 melB CDS
Automated Process Uncurated Go view Pantoea ananatis PA13 APK-0000874 CDS
Automated Process Uncurated Go view Pantoea stewartii DC283 ACV-0291218 melB CDS
Automated Process Uncurated Go view Salmonella Agona SL483 BBX-0008724 CDS
Automated Process Uncurated Go view Salmonella Arizonae CDC 346-86 BCD-0003518 CDS
Person Uncurated Go view Salmonella Choleraesuis SC-B67 ADB-0004918 melB CDS
Automated Process Uncurated Go view Salmonella Dublin CT_02021853 BCB-0008933 CDS
Automated Process Uncurated Go view Salmonella Enteritidis P125109 BCF-0013484 melB CDS
Automated Process Uncurated Go view Salmonella Gallinarum 287/91 BCG-0012750 melB CDS
Automated Process Uncurated Go view Salmonella Hadar RI_05P066 BBT-0007796 CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL476 BBV-0008999 CDS
Automated Process Uncurated Go view Salmonella Heidelberg SL486 BBW-0007405 CDS
Automated Process Uncurated Go view Salmonella Javiana GA_MM04042433 BCA-0004039 CDS
Automated Process Uncurated Go view Salmonella Kentucky CDC 191 BBZ-0005781 CDS
Automated Process Uncurated Go view Salmonella Kentucky CVM29188 BBP-0008108 CDS
Automated Process Uncurated Go view Salmonella Newport SL254 BBQ-0008990 CDS
Automated Process Uncurated Go view Salmonella Newport SL317 BBR-0008296 CDS
Automated Process Uncurated Go view Salmonella Paratyphi A AKU_12601 BCH-0004152 CDS
Person Uncurated Go view Salmonella Paratyphi A ATCC 9150 ADA-0004112 melB CDS
Automated Process Uncurated Go view Salmonella Paratyphi B SPB7 BCC-0005326 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA23 BBN-0004342 CDS
Automated Process Uncurated Go view Salmonella Saintpaul SARA29 BBM-0007445 CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund CVM19633 BBO-0008616 CDS
Automated Process Uncurated Go view Salmonella Schwarzengrund SL480 BBY-0006306 CDS
Person Approved Go view Salmonella Typhi CT18 ABX-0114546 melB CDS
Person Uncurated Go view Salmonella Typhi Ty2 ABS-0085933 melB CDS
Automated Process Uncurated Go view Salmonella Typhimurium 14028S AJR-0005178 melB CDS
Person Uncurated Go view Salmonella Typhimurium LT2 [T] ABU-0103245 melB CDS
Automated Process Uncurated Go view Salmonella Virchow SL491 BBS-0004844 CDS
Automated Process Uncurated Go view Salmonella Weltevreden SL484 BBU-0007513 CDS
Automated Process Uncurated Go view Salmonella enterica CVM23701 BBL-0005821 CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0004690 melB CDS
Person Approved Go view Shigella flexneri 2457T ABQ-0074844 melB CDS
Person Approved Go view Shigella flexneri 301 ABT-0090318 melB CDS
Automated Process Approved Go view Shigella flexneri 8401 BAG-0004366 melB CDS
Automated Process Uncurated Go view Yersinia intermedia ATCC 29909 [T] AEH-0001130 melB2_1 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 43003 APO-0013914 CDS
Automated Process Uncurated Go view Yokenella regensburgei ATCC 49455 [T] AMG-0003735 CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB21687mutantTn5 InsertionRequest the StrainABE-0013495melBmelB::Tn5KAN-I-SceI at position 113 in Minus orientation ,+ pKD460Blattner Laboratory
FB21688mutantTn5 InsertionRequest the StrainABE-0013495melBmelB::Tn5KAN-I-SceI at position 113 in Minus orientation ,- pKD465Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 4338369 to 4349790
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0013483adiACDScomplement43382544340521Upstream
ABE-0013486melRCDScomplement43407204341628Upstream
ABE-0013493melACDSforward43419114343266Upstream
ABE-0013495melBCDSforward43433694344790Matches
ABE-0285680repeat_regionforward43448154344909Downstream
ABE-0013498yjdFCDScomplement43449294345558Downstream
ABE-0013501fumBCDScomplement43456804347326Downstream
ABE-0013503dcuBCDScomplement43474044348744Downstream
ABE-0013507dcuRCDScomplement43493154350034Downstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.