Escherichia coli K-12 MG1655
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Working With: Genome: Escherichia coli K-12 MG1655 Version: v3

ABE-0014067 (fecA) - Escherichia coli K-12 MG1655 - Feature Annotation

ASAP ID
ABE-0014067 showshow history
Names
fecA JW4251 ECK4281 b4291
Product
KpLE2 phage-like element; ferric citrate outer membrane transporter
Type
CDS
Location
# Contig Strand Left Right Length (b.p.) Length (a.a.)
1 Chromosome complement 4514353 4516677 2325 775
Sequence

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Context

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Protein Families to toptop
Author Approval Family Annotated
Automated Process Approved IPR012910 (IPR012910 TonB-dependent receptor, plug domain) Apr 2008
Automated Process Approved IPR000531 (IPR000531 TonB-dependent receptor-like, beta-barrel) Apr 2008
Automated Process Approved IPR010916 (IPR010916 TonB box, conserved site) Apr 2008
Automated Process Approved IPR010917 (IPR010917 TonB-dependent receptor, conserved site) Apr 2008
Automated Process Approved IPR010105 (IPR010105 TonB-dependent siderophore receptor) Apr 2008
Automated Process Approved IPR011662 (IPR011662 Secretin/TonB, short N-terminal domain) Apr 2008
Automated Process Approved PF07715 (PF07715 TonB-dependent Receptor Plug Domain) Jul 2018
Automated Process Approved PF07660 (PF07660 Secretin and TonB N terminus short domain) Jul 2018
Automated Process Approved PF00593 (PF00593 TonB dependent receptor) Jul 2018
Gene Ontology to toptop
Author Approval Type Annotation Evidence Annotated
Automated Process Approved GO biological process GO:0009935 (OBSOLETE) nutrient import GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR011662
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Person Uncurated GO biological process GO:0006826 iron ion transport Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0015891 siderophore transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR010105
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0006810 transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000531
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO cellular component GO:0019867 outer membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR011662
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000531
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0009279 cell outer membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated GO cellular component GO:0009279 cell outer membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO molecular function GO:0015343 siderophore transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR010105
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO molecular function GO:0038023 signaling receptor activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR010105
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0005215 transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000531
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0004872 (DEPRECATED) receptor activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000531
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0005506 iron ion binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR010105
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Annotations to toptop
Author Approval Type Annotation Evidence Annotated
Nomenclature
Person Approved name fecA Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Person Approved synonym ECK4281 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved synonym JW4251 Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved locus tag b4291 Unique Identifier - Origin: Guy Plunkett III, guy@genome.wisc.edu May 2004
Product
Person Approved product KpLE2 phage-like element; ferric citrate outer membrane transporter Published Annotation - PubMed ID: 16397293 Jan 2006
Person Approved alternate product name outer membrane receptor; citrate-dependent iron transport, outer membrane receptor Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Jan 2006
Person Uncurated alternate product name KpLE2 phage-like element; outer membrane porin, receptor for ferric citrate, in multi-component regulatory system with cytoplasmic FecI (sigma factor) and membrane-bound FecR Published Annotation - Database Name: GenProtEC Jan 2006
Protein Family
Automated Process Approved protein family IPR011662 Secretin/TonB, short N-terminal domain Protein Sequence Similarity - InterPro Domain: IPR011662
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR010916 TonB box, conserved site Protein Sequence Similarity - InterPro Domain: IPR010916
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF07660 Secretin and TonB N terminus short domain Protein Sequence Similarity - PFAM Model Name: PF07660
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family PF00593 TonB dependent receptor Protein Sequence Similarity - PFAM Model Name: PF00593
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR010105 TonB-dependent siderophore receptor Protein Sequence Similarity - InterPro Domain: IPR010105
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family PF07715 TonB-dependent Receptor Plug Domain Protein Sequence Similarity - PFAM Model Name: PF07715
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved protein family IPR010917 TonB-dependent receptor, conserved site Protein Sequence Similarity - InterPro Domain: IPR010917
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR012910 TonB-dependent receptor, plug domain Protein Sequence Similarity - InterPro Domain: IPR012910
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved protein family IPR000531 TonB-dependent receptor-like, beta-barrel Protein Sequence Similarity - InterPro Domain: IPR000531
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Other
Person Uncurated MultiFun 6.1 (cell structure; membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 7.4 (location of gene products; outer membrane) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 4.S.95 (transport; substrate; iron dicitrate) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 4.1.B.14 (transport; Channel-type Transporters; Beta barrel porins (The Outer Membrane Porin (OMP) Functional Superfamily); The Outer Membrane Receptor (OMR) Family) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Person Uncurated MultiFun 5.5.7 (cell processes; adaptation to stress; Fe aquisition) Published Annotation - MultiFun Database Name: MultiFun Feb 2002
Automated Process Approved codon start 1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref EcoGene:EG10286 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GeneID:946427 Published Annotation - Entrez Gene Database ID: 946427
Reference: Escherichia coli K-12 MG1655 		Jun 2009
Person Approved db xref UniProtKB:P13036 Published Annotation - Swiss-Prot Accession Number: P13036 Jan 2005
Automated Process Approved db xref UniProtKB/Swiss-Prot:P13036 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref GI:1790743 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved db xref ASAP:ABE-0014067 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Person Approved function membrane; Transport of small molecules: Cations Published Annotation - GenBank Accession Number: U00096
Reference: Escherichia coli MG1655 		Oct 1998
Automated Process Approved note outer membrane receptor; citrate-dependent iron transport, outer membrane receptor; GO_component: GO:0009274 - peptidoglycan-based cell wall; GO_component: GO:0009279 - cell outer membrane; GO_process: GO:0006826 - iron ion transport Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Approved protein id AAC77247.1 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Automated Process Uncurated subcellular localization Outer Membrane Predicted by PSORTb - PSORTb Final_Score: 10.00
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated subsystem component of in silico model iAF1260 Experimental - PubMed ID: 17593909
Reference: Escherichia coli K-12 MG1655 		Jan 2009
Automated Process Approved transl table 11 Published Annotation - GenBank Accession Number: U00096.3
Reference: Escherichia coli K-12 MG1655 		Oct 2013
Gene Ontology
Person Uncurated GO biological process GO:0006826 iron ion transport Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO biological process GO:0015891 siderophore transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR010105
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0009935 (OBSOLETE) nutrient import GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR011662
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO biological process GO:0006810 transport GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000531
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO cellular component GO:0009279 cell outer membrane GO Inferred from Electronic Annotation - PubMed ID: 15501914
Reference: Escherichia coli K-12 MG1655 		Feb 2008
Person Uncurated GO cellular component GO:0009279 cell outer membrane Ontology Mapping - Source and Dest Ontology: MultiFun to GO Mar 2004
Automated Process Approved GO cellular component GO:0016020 membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000531
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO cellular component GO:0019867 outer membrane GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR011662
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Uncurated GO molecular function GO:0038023 signaling receptor activity GO Inferred from Electronic Annotation - Database Name:Identifier: InterPro2GO: IPR010105
Reference: Escherichia coli K-12 MG1655 		Jul 2018
Automated Process Approved GO molecular function GO:0015343 siderophore transmembrane transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR010105
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0005506 iron ion binding GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR010105
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0005215 transporter activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000531
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Automated Process Approved GO molecular function GO:0004872 (DEPRECATED) receptor activity GO Inferred from Electronic Annotation - Database Name:Identifier: INTERPRO:IPR000531
Reference: Escherichia coli K-12 MG1655 		Apr 2008
Comparative to toptop
Author Approval Details Genome Feature Name Type
Homolog
Person Approved Go view Shigella sonnei 046 ADD-0004847 fecA CDS
Member
Automated Process Uncurated Go view OrthoMCL (14 enterobacteria) AMM-0004668 CDS
Automated Process Uncurated Go view OrthoMCL (205 gammaproteobacteria) APP-0004703 fecA CDS
Automated Process Uncurated Go view OrthoMCL (307 Enterobacteriaceae) ATM-0004421 fecA CDS
Automated Process Uncurated Go view OrthoMCL (42 Serratia) BEZ-0002832 fecA CDS
Automated Process Uncurated Go view OrthoMCL (UW draft) CQJ-0004205 fecA CDS
Ortholog
Automated Process Uncurated Go view Citrobacter freundii ATCC 8090 [T] AVI-0002425 fecA CDS
Automated Process Uncurated Go view Erwinia tasmaniensis Et1/99 [T] AFI-0003140 fecA CDS
Automated Process Uncurated Go view Escherichia coli 101-1 (EAEC) BAB-0006897 fecA CDS
Automated Process Uncurated Go view Escherichia coli ATCC 11775 [T] AMC-0004453 CDS
Automated Process Uncurated Go view Escherichia coli ATCC 8739 AEM-0006210 CDS
Automated Process Uncurated Go view Escherichia coli B171 (EPEC) ADN-0006013 fecA CDS
Automated Process Uncurated Go view Escherichia coli B7A (ETEC) ADK-0007365 FecA CDS
Automated Process Uncurated Go view Escherichia coli E110019 (EPEC) ADM-0008296 fecA CDS
Automated Process Uncurated Go view Escherichia coli E24377A (ETEC) ADO-0009304 fecA CDS
Person Uncurated Go view Escherichia coli K-12 DH10B AEC-0003966 fecA CDS
Person Uncurated Go view Escherichia coli K-12 W3110 ADY-0004384 fecA CDS
Person Uncurated Go view Hafnia alvei ATCC 13337 [T] AMD-0002728 CDS
Automated Process Uncurated Go view Leclercia adecarboxylata ATCC 23216 [T] AME-0001627 CDS
Automated Process Uncurated Go view Pantoea sp. At-9b AGM-0006852 CDS
Person Uncurated Go view Pectobacterium atrosepticum SCRI1043 ABL-0061323 fecA CDS
Automated Process Uncurated Go view Pectobacterium brasiliensis 1692 AED-0000598 fecA CDS
Person Uncurated Go view Photorhabdus luminescens subsp. laumondii TTO1 [T] ACD-0146904 fecA CDS
Person Uncurated Go view Pseudomonas syringae pv. tomato DC3000 ADG-0001236 CDS
Automated Process Uncurated Go view Serratia marcescens subsp. marcescens ATCC 13880 [T] AMF-0003501 CDS
Automated Process Uncurated Go view Shigella dysenteriae 1012 BAA-0004835 fecA CDS
Person Uncurated Go view Shigella dysenteriae 197 ADU-0004847 fecA CDS
Automated Process Uncurated Go view Shigella sonnei 046 ADD-0004847 fecA CDS
Person Uncurated Go view Xenorhabdus nematophila ATCC 19061 [T] ADW-0006671 fecA CDS
Automated Process Uncurated Go view Yersinia enterocolitica 8081 BAH-0001691 CDS
Experimental to toptop
SelectSetNameSetDetailsNotes
stringent responsetranscriptional profile of the stringent responseNone
Recombinant heat-shock dataexpression data for recombinant strain under heat shockThese data are from the Harcum laboratory. (Jeremy Glasner)
Suppressor tRNA expressionexperiment comparing transcript levels in cells with and without suppressor tRNA expressionThese data were reported in the paper: Herring and Blattner. 2004. Global transcriptional effects of a suppressor tRNA and the inactivation of the regulator frmR. J. Bacteriol. 186(20):6714-20. Link to Abstract (Jeremy Glasner)
Recombinant E. coli expressionexperiment comparing expression of wild-type E. coli with the same strain harboring a plasmid that expresses protein when induced with IPTG These data were reported in the paper: F. T. Haddadin, S. W. Harcum (2005) Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli, Biotechnology and Bioengineering, 90:127-153. Link to Abstract (Jeremy Glasner)
Aerobic shiftAerobic shiftThese data were reported in the paper: Covert MW, Knight EM, Reed JL, Herrgard MJ, Palsson BO. 2004. Integrating high-throughput and computational data elucidates bacterial networks. Nature. 2004 May 6;429(6987):92-6. Link to Abstract (Jeremy Glasner)
CalibratorCalibrated microarray experiments (50 hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
AffyData1Affymetrix Experiments (data from 41 chip hybridizations)This data was used in the paper: Allen et al., J. Bacteriol. 185(21):6392-9 (Jeremy Glasner)
Strains to toptop

Strains with mutations in this feature.

Strain NameStrain TypeStrain InformationStrain RequestFeatureIDNameMutation DetailsExperiments Using StrainSubmitter
FB22962mutantTn5 InsertionRequest the StrainABE-0014067fecAfecA::Tn5(KAN-I-SceI) at position 1764 in Plus orientation ,+ pKD46 0Blattner Laboratory
Context Table to toptop

Listed below are features that lie nearby this feature. Features encoded in multiple intervals may appear more than once in the table. Features that span the origin will have a left endpoint greater than the right endpoint.

Features overlapping the region 4509353 to 4521677
FeatureIDNameFeature TypeStrandLeft EndRight EndRelationship
ABE-0000001sourceforward14641652Contains
ABE-0234214KpLE2prophageforward44965404536609Contains
ABE-0014050insO-2CDSpforward45089584509554Downstream
ABE-0285254insOpseudogeneforward45090074509771(Interval #2) Downstream
ABE-0014051mobile_elementforward45090074509801Downstream
ABE-0285083yjhWCDSpforward45095514509793Downstream
ABE-0014055yjhVpseudogeneforward45098044510133Downstream
ABE-0014057fecECDScomplement45106904511457Downstream
ABE-0014059fecDCDScomplement45114584512414Downstream
ABE-0014063fecCCDScomplement45124114513409Downstream
ABE-0014065fecBCDScomplement45134064514308Downstream
ABE-0014067fecACDScomplement45143534516677Matches
ABE-0014070fecRCDScomplement45167644517717Upstream
ABE-0014072fecICDScomplement45177144518235Upstream
ABE-0285747ykiECDSforward45183724518440Upstream
ABE-0014074mobile_elementforward45184724519239Upstream
ABE-0014077insA-7CDSforward45185274518802Upstream
ABE-0285084insB-7_1CDSpforward45187214519014Upstream
ABE-0285109insB-7pseudogeneforward45187214519224Upstream
ABE-0285085insB-7_2CDSpforward45190154519224Upstream
ABE-0014079yjhUCDScomplement45193384520324Upstream
ABE-0014084yjhFCDScomplement45206714522020Upstream
Release Notes to toptop
All MG1655 annotations and sequence data are publicly available, and investigators can use these data in publications without contacting the genome curators. This version of the sequence was the source of GenBank record U00096.3. Ongoing annotation updates to that GenBank record are being generated from a collaboration that includes ASAP, EcoGene, the Coli Genetic Stock Center, EcoliHub, EcoCyc, RegulonDB and UniProtKB/Swiss-Prot.