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Synthetic circuit of inositol phosphorylceramide synthase in Leishmania: a chemical biology approach

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Journal of Chemical Biology

Abstract

Building circuits and studying their behavior in cells is a major goal of systems and synthetic biology. Synthetic biology enables the precise control of cellular states for systems studies, the discovery of novel parts, control strategies, and interactions for the design of robust synthetic systems. To the best of our knowledge, there are no literature reports for the synthetic circuit construction for protozoan parasites. This paper describes the construction of genetic circuit for the targeted enzyme inositol phosphorylceramide synthase belonging to the protozoan parasite Leishmania. To explore the dynamic nature of the circuit designed, simulation was done followed by circuit validation by qualitative and quantitative approaches. The genetic circuit designed for inositol phosphorylceramide synthase (Biomodels Database—MODEL1208030000) shows responsiveness, oscillatory and bistable behavior, together with intrinsic robustness.

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Acknowledgments

The authors would like to thank Dr. SC Mande, Director NCCS for supporting the Bioinformatics and High Performance Computing Facility. Vineetha Mandlik acknowledges the financial support as Junior Research Fellow of Department of Biotechnology, Government of India. The work was supported by the Department of Biotechnology, New Delhi, Govt. of India.

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Authors and Affiliations

  1. National Centre for Cell Science, NCCS Complex, Ganeshkhind, Pune University Campus, Pune, 411007, India

    Vineetha Mandlik, Sonali Shinde, Milsee Mol & Shailza Singh

  2. Department of Computer Science and Technology, Sardar Patel University, Vallabh Vidyanagar, India

    Dixita Limbachiya

Authors
  1. Vineetha Mandlik
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  2. Dixita Limbachiya
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  3. Sonali Shinde
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  4. Milsee Mol
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  5. Shailza Singh
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Correspondence to Shailza Singh.

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Mandlik, V., Limbachiya, D., Shinde, S. et al. Synthetic circuit of inositol phosphorylceramide synthase in Leishmania: a chemical biology approach. J Chem Biol 6, 51–62 (2013). https://doi.org/10.1007/s12154-012-0089-7

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  • DOI: https://doi.org/10.1007/s12154-012-0089-7

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