KEGG ENZYME: 1.5.3.13

ENZYME: 1.5.3.13

Entry

EC 1.5.3.13                 Enzyme

Name

N1-acetylpolyamine oxidase;
hPAO-1;
PAO (ambiguous);
mPAO;
hPAO;
polyamine oxidase (ambiguous)

Class

Oxidoreductases;
Acting on the CH-NH group of donors;
With oxygen as acceptor

Sysname

N1-acetylpolyamine:oxygen oxidoreductase (3-acetamidopropanal-forming)

Reaction(IUBMB)

(1) N1-acetylspermidine + O2 + H2O = putrescine + 3-acetamidopropanal + H2O2 [RN:R09074];
(2) N1-acetylspermine + O2 + H2O = spermidine + 3-acetamidopropanal + H2O2 [RN:R03899]

Reaction(KEGG)

R03899 R09074

Substrate

N1-acetylspermidine [CPD:C00612];
O2 [CPD:C00007];
H2O [CPD:C00001];
N1-acetylspermine [CPD:C02567]

Product

putrescine [CPD:C00134];
3-acetamidopropanal [CPD:C18170];
H2O2 [CPD:C00027];
spermidine [CPD:C00315]

Comment

The enzyme also catalyses the reaction: N1,N12-diacetylspermine + O2 + H2O = N1-acetylspermidine + 3-acetamamidopropanal + H2O2 [1]. No or very weak activity with spermine, or spermidine in absence of aldehydes. In presence of aldehydes the enzyme catalyses the reactions: 1. spermine + O2 + H2O = spermidine + 3-aminopropanal + H2O2, and with weak efficiency 2. spermidine + O2 + H2O = putrescine + 3-aminopropanal + H2O2 [2]. A flavoprotein (FAD). This enzyme, encoded by the PAOX gene, is found in mammalian peroxisomes and oxidizes N1-acetylated polyamines at the exo (three-carbon) side of the secondary amine, forming 3-acetamamidopropanal. Since the products of the reactions are deacetylated polyamines, this process is known as polyamine back-conversion. Differs in specificity from EC 1.5.3.14 [polyamine oxidase (propane-1,3-diamine-forming)], EC 1.5.3.15 [N8-acetylspermidine oxidase (propane-1,3-diamine-forming)], EC 1.5.3.16 (spermine oxidase) and EC 1.5.3.17 (non-specific polyamine oxidase).

History

EC 1.5.3.13 created 2009

Orthology

K00308

N1-acetylpolyamine oxidase

Genes

HSA:	196743(PAOX)

PTR:	100612198(PAOX)

PPS:	100991166(PAOX)

GGO:	101144152(PAOX)

PON:	100447841(PAOX)

NLE:	100591252(PAOX)

HMH:	116481595(PAOX)

MCC:	100498675(PAOX)

MCF:	102133238(PAOX)

MTHB:

126963296

MNI:	105471252(PAOX)

CSAB:

103245972(PAOX)

CATY:

105598981(PAOX)

PANU:

101023302(PAOX)

TGE:	112632181(PAOX)

MLEU:

105540279(PAOX)

RRO:	104677646(PAOX)

RBB:	108543375(PAOX)

TFN:	117082665(PAOX)

PTEH:

111522070(PAOX)

CANG:

105511787(PAOX)

CJC:	100391148(PAOX)

SBQ:	101035835(PAOX)

CIMI:

108297208(PAOX)

CSYR:

103252832(PAOX)

MMUR:

105861485(PAOX)

LCAT:

123650190(PAOX)

PCOQ:

105823475(PAOX)

OGA:	100956508(PAOX)

MMU:	212503(Paox)

MCAL:

110298329(Paox)

MPAH:

110325417(Paox)

RNO:	293589(Paox)

MCOC:

116103172(Paox)

ANU:	117713570(Paox)

MUN:	110563321(Paox)

CGE:	100754371(Paox)

MAUA:

101823694(Paox)

PROB:

127228505(Paox)

PLEU:

114693348(Paox)

MORG:

121458151(Paox)

MFOT:

126498369

AAMP:

119810252(Paox)

NGI:	103725840(Paox)

HGL:	101697041(Paox)

CPOC:

100725437(Paox)

CCAN:

109698400(Paox)

DORD:

106000243(Paox)

DSP:	122127835(Paox)

PLOP:

125345917(Paox)

NCAR:

124974917

MMMA:

125612746(Paox)

OCU:

100341296

OPI:	101523414(PAOX)

TUP:	102487097(PAOX)

GVR:	103583528 103606759(PAOX)

CFA:	480798(PAOX)

CLUD:

112672549(PAOX)

VVP:	112928783(PAOX)

VLG:	121485165(PAOX)

NPO:	129493938(PAOX)

AML:	100470303(PAOX)

UMR:	103659644(PAOX)

UAH:	113252100(PAOX)

UAR:	123800009(PAOX)

ELK:

111145128

LLV:

125084595

MPUF:

101685018(PAOX)

MNP:	132016910(PAOX)

MLK:	131830493(PAOX)

NVS:	122901244(PAOX)

ORO:	101366439(PAOX)

EJU:	114221820(PAOX)

ZCA:	113923417(PAOX)

MLX:	117998087(PAOX)

NSU:	110578814(PAOX)

LWW:

102730987

FCA:	101090142(PAOX)

PYU:	121037434(PAOX)

PCOO:

112854712(PAOX)

PBG:	122493419(PAOX)

PVIV:

125147879(PAOX)

LRUF:

124507260

PTG:	102954249(PAOX)

PPAD:

109256703(PAOX)

PUC:

125933073

AJU:

106987504

HHV:	120245361(PAOX)

BTA:	282639(PAOX)

BOM:	102280842(PAOX)

BIU:	109553024(PAOX)

BBUB:

102390709(PAOX)

BBIS:

105000957(PAOX)

CHX:	102185531(PAOX)

OAS:	101111594(PAOX)

BTAX:

128067852(PAOX)

ODA:	120873814(PAOX)

CCAD:

122445802(PAOX)

MBEZ:

129555223(PAOX)

SSC:	100626281(PAOX)

CFR:	102509887(PAOX)

CBAI:

105066940(PAOX)

CDK:	105104593(PAOX)

VPC:	102545469(PAOX)

BACU:

103020663(PAOX)

BMUS:

118882622(PAOX)

LVE:	103087537(PAOX)

OOR:	101276665(PAOX)

DLE:	111172154(PAOX)

PCAD:

112061830(PAOX)

PSIU:

116741957(PAOX)

NASI:

112411064(PAOX)

ECB:	100629322(PAOX)

EPZ:	103556402(PAOX)

EAI:	106841087(PAOX)

MYD:	102769158(PAOX)

MMYO:

118669673(PAOX)

MLF:	102426319(PAOX)

PKL:	118719550(PAOX)

EFUS:

103288607(PAOX)

MNA:	107532071(PAOX)

DRO:	112300229(PAOX)

SHON:

118990591(PAOX)

AJM:	119059480(PAOX)

PDIC:

114498062(PAOX)

PHAS:

123828962(PAOX)

MMF:	118632237(PAOX)

PPAM:

129079542(PAOX)

HAI:	109383553(PAOX)

RFQ:	117036460(PAOX)

PALE:

102897106(PAOX)

PGIG:

120581210(PAOX)

PVP:	105300392(PAOX)

RAY:	107517150(PAOX)

MJV:	108395414(PAOX)

TOD:	119234105(PAOX)

SARA:

101541643(PAOX)

LAV:	100671217(PAOX)

TMU:

101340288

ETF:	101656981(PAOX)

DNM:	101447322(PAOX)

MDO:	100618593(PAOX)

GAS:	123236473(PAOX)

SHR:	100935074(PAOX)

PCW:	110214046(PAOX)

OAA:	100091298(PAOX)

GGA:	100857693(PAOX)

PCOC:

116234771(PAOX)

CJO:	107315627(PAOX)

TPAI:

128077490(PAOX)

LMUT:

125693791(PAOX)

NMEL:

110390485(PAOX)

APLA:

110352281(PAOX)

ACYG:

125183405(PAOX)

CATA:

118245128(PAOX)

AFUL:

116491120(PAOX)

TGU:	100226026(PAOX)

LSR:	110474029(PAOX)

SCAN:

103814080(PAOX)

PMOA:

120499686(PAOX)

OTC:	121336117(PAOX)

PRUF:

121357498(PAOX)

FAB:	101813552(PAOX)

OMA:	130255143 130255144 130255153

PHI:	106628584(PAOX)

PMAJ:

107207134(PAOX)

CCAE:

111944263

CCW:	120410203(PAOX)

ETL:	114067585(PAOX)

ACHL:

103807741(PAOX)

SVG:	106856738(PAOX)

MMEA:

130585749(PAOX)

HRT:	120755748(PAOX)

FPG:	101915114(PAOX)

FCH:	102054181(PAOX)

CLV:	102084101(PAOX)

EGZ:	104125146(PAOX)

NNI:	104013577(PAOX)

PCRI:

104037035(PAOX)

PLET:

104616531(PAOX)

EHS:	104516221(PAOX)

ACUN:

113481861(PAOX)

TALA:

104360241(PAOX)

PADL:

103924291(PAOX)

AFOR:

103895502(PAOX)

ACHC:

115348029(PAOX)

HLE:	104830800(PAOX)

AGEN:

126043044

GCL:

127017995

CCRI:

104163299(PAOX)

CSTI:

104560256(PAOX)

MUI:	104546220(PAOX)

LDI:	104347823(PAOX)

MNB:	103771648(PAOX)

OHA:	104330391(PAOX)

NNT:	104405783(PAOX)

SHAB:

115608051(PAOX)

DPUB:

104310247(PAOX)

PGUU:

104464607(PAOX)

CPEA:

104397767

AVIT:

104279933

CVF:	104283440(PAOX)

RTD:	128910990(PAOX)

CUCA:

104060051(PAOX)

TEO:	104381259(PAOX)

BRHI:

104490004

AAM:	106495312(PAOX)

AROW:

112977219(PAOX)

NPD:	112948426(PAOX)

TGT:

104566853

DNE:	112981625(PAOX)

SCAM:

104149533(PAOX)

ASN:	102375090(PAOX)

AMJ:	102575603(PAOX)

PSS:	102444520(PAOX)

CMY:	102934388(PAOX)

CCAY:

125640517(PAOX)

DCC:	119859151(PAOX)

CPIC:

101935511(PAOX)

TST:	117879745(PAOX)

CABI:

116816949(PAOX)

MRV:	120409350(PAOX)

ACS:	100563036(paox)

ASAO:

132771392(PAOX)

PVT:	110087238(PAOX)

SUND:

121925721(PAOX)

PBI:	103061034(PAOX)

PMUR:

107291447(PAOX)

CTIG:

120309930(PAOX)

TSR:	106548466(PAOX)

PGUT:

117664658(PAOX)

APRI:

131199181(PAOX)

PTEX:

113449395(PAOX)

NSS:	113425187(PAOX)

VKO:	123017482(PAOX)

PMUA:

114596979(PAOX)

PRAF:

128413639(PAOX)

ZVI:	118096470(PAOX)

HCG:	128348974(PAOX)

GJA:	107106972(PAOX)

STOW:

125437519(PAOX)

EMC:	129332520(PAOX)

XLA:	108701271(XB5760632.L) 495472(paox.L)

XTR:	100145496 496840(paox)

NPR:	108792302 108793155(PAOX)

RTEM:

120909112(PAOX) 120944159

BBUF:

121003238 121003667 121003668 121005653 121007382

BGAR:

122940929 122940974 122941735 122942437 122945743

MUO:	115470211(PAOX) 115474152

GSH:	117359663(PAOX) 117361265

DRE:	110440136 562105(paox)

SRX:	107708014 107757157

SANH:

107668834 107673908

SGH:	107564350 107588698

CCAR:

109082582 109100677

CAUA:

113056900(paox)

CGIB:

127970021

PTET:

122356100

LROH:

127175422

OMC:

131552151

PPRM:

120479786(si:dkey-275b16.2)

MAMB:

125277027(paox)

CIDE:

127525230

MASI:

127414290

TROS:

130559126(paox)

TDW:	130431658 130431659

MANU:

129416577(paox)

IPU:	108263770(paox)

IFU:	128605642(paox)

PHYP:

113544009(paox)

SMEO:

124376222(paox)

TFD:	113639737(paox)

TVC:	132847319(paox)

AMEX:

103029926(paox)

CMAO:

118810430(si:dkey-275b16.2)

EEE:	113580961(paox)

CHAR:

105912519(paox)

TRU:	101066188 101066416 115249509

TFS:	130533684 130533686

LCO:	104924262(paox)

NCC:	104961348(paox)

TBEN:

117499469 117499470

CGOB:

115019948(paox)

PGEO:

117459470 117459471

GACU:

117536785

EMAC:

134858483 134858484

ELY:	117248216 117248217 117248218

EFO:	125903177 125903178 125903180

PLEP:

121954868(paox) 121961702 121966912

SLUC:

116053243(si:dkey-275b16.2)

ECRA:

117960224(si:dkey-275b16.2)

ESP:	116704970(paox)

PFLV:

114571689(paox)

GAT:	120820262(si:dkey-275b16.2)

PPUG:

119214616(si:dkey-275b16.2)

AFB:	129092355(paox)

CLUM:

117744444(si:dkey-275b16.2)

PSWI:

130207028(paox)

MSAM:

119916864 119916865

SCHU:

122884600

CUD:	121511073 121511074

MZE:	101482825 101483098

ONL:	100690986 102079225 102080354

OAU:	116309827 116322734 116322735 120443320

OLA:	101166491 101169480

OML:	112162019 112162020

CSAI:

133463263(paox)

XMA:	102221096 102221362 102229573 102231116(paox)

XCO:	114137796 114137797

XHE:	116713685(paox) 116713686 116713687 116713688 116713690 116713691

PRET:

103476962 103476963

PFOR:

103134031 103134033

PLAI:

106944797 106944798

PMEI:

106915137 106915139

GAF:	122842299 122842301

PPRL:

129360064 129360065

CVG:	107086571(paox) 107086572

CTUL:

119790662 119790698

GMU:	124859080 124859081 124859082

NFU:	107375621(paox) 107375622

KMR:	108234132(si:dkey-275b16.2)

ALIM:

106521929(paox) 106522054

NWH:	119411991 119412262

AOCE:

111577441(paox) 111577442

MCEP:

125018348(paoX)

CSEM:

103387348(paox)

POV:	109636227(paox) 109636235

SSEN:

122781818 122782093

HHIP:

117775952 117775953

HSP:	118119312 118119314

SMAU:

118283923 118284107

LCF:	108888274 108888275

SDU:	111234316 111234324

SLAL:

111658171 111658178

XGL:	120796063(si:dkey-275b16.2)

HCQ:	109516209(paox)

SSCV:

125978397

SBIA:

133510176(paox)

PEE:	133409642(paox)

PTAO:

133485414(paox)

BPEC:

110157178(paox)

MALB:

109961140 109961142

BSPL:

114841752 114841755

SJO:	128372986(paox)

SASA:

106576873(paox)

STRU:

115194051(paox)

OTW:	112252755(paox)

OMY:	110525829(si:dkey-275b16.2)

OGO:	124041480(paox)

ONE:	115135354(paox)

OKI:	109889726(paox)

OKE:	118369457(paox)

SALP:

112070947(paox)

SNH:	120017490(si:dkey-275b16.2)

CCLU:

121553303(paox)

ELS:	105010685(paox)

SFM:	108918131(paox)

PKI:	111850322(paox)

AANG:

118218197

LOC:	102690126(paox) 102690282

PSPA:

121325794

ARUT:

117405323(zgc:66484) 117416104 117417775

PSEX:

120531224(zgc:66484) 120541829(si:dkey-275b16.2)

LCM:	102353427(PAOX)

CMK:	103178487 103182165(paox)

RTP:	109923723(paox)

CPLA:

122541827(paox)

HOC:	132818167 132818168 132834987(paox)

LERI:

129700605 129712837(paox)

PMRN:

116938876 116941302(PAOX)

LRJ:	133345556(PAOX) 133346070

BBEL:

109475433 109475523

SPU:	579633 593351

LPIC:

129272787

BCOO:

119070647

ALAB:

122712989

NVL:

108562895

PPYR:

116170494

OTU:

111414030

BMAN:

114248385

MSEX:

115449390

BANY:

112051110

MJU:

123869443

VCD:

124531377

MCIX:

123667587

PPOT:

106099637

PXU:

106118355

PRAP:

110999660

LSIN:

126968921

HAW:

110371916

SLIU:

111352505

OFU:

114354554

PGW:

126367107

CCRN:

123291954

ACOO:

126844733 126844783

DVT:

126897977

CLEC:

106660790 106669297

HHAL:

106681969 106692474

HVI:

124355287

MQU:

128996794

FOC:

113201965

TPAL:

117642117

DMK:

116926824

PVM:

113805206

PJA:	122244577 122248719 122265719

PCHN:

125028879 125028930

HAME:

121878335

PCLA:

123756333

CQD:

128690821

PTRU:

123516696

ESN:

126999731

LSM:

121120666

TCF:

131885400

RMP:

119169577

LPOL:

106471999 106472000

PMEO:

129592673 129597833

GAE:

121368825

HRF:

124123005

HRJ:

124276736

CRG:

117681267

PMAX:

117328961

DPOL:

127866284 127866285

PFJ:	MYCFIDRAFT_194913

FFU:	CLAFUR5_12781

BCOM:

BAUCODRAFT_118878

GTT:	GUITHDRAFT_110590

» show all

Reference

1 [PMID:12477380]

Authors

Vujcic S, Liang P, Diegelman P, Kramer DL, Porter CW

Title

Genomic identification and biochemical characterization of the mammalian polyamine oxidase involved in polyamine back-conversion.

Journal

Biochem J 370:19-28 (2003)
DOI:10.1042/BJ20021779

Sequence

[hsa:196743]

Reference

2 [PMID:15611107]

Authors

Jarvinen A, Grigorenko N, Khomutov AR, Hyvonen MT, Uimari A, Vepsalainen J, Sinervirta R, Keinanen TA, Vujcic S, Alhonen L, Porter CW, Janne J

Title

Metabolic stability of alpha-methylated polyamine derivatives and their use as substitutes for the natural polyamines.

Journal

J Biol Chem 280:6595-601 (2005)
DOI:10.1074/jbc.M412788200

Reference

3 [PMID:15791459]

Authors

Wang Y, Hacker A, Murray-Stewart T, Frydman B, Valasinas A, Fraser AV, Woster PM, Casero RA Jr

Title

Properties of recombinant human N1-acetylpolyamine oxidase (hPAO): potential role in determining drug sensitivity.

Journal

Cancer Chemother Pharmacol 56:83-90 (2005)
DOI:10.1007/s00280-004-0936-5

Reference

4 [PMID:12660232]

Authors

Wu T, Yankovskaya V, McIntire WS

Title

Cloning, sequencing, and heterologous expression of the murine peroxisomal flavoprotein, N1-acetylated polyamine oxidase.

Journal

J Biol Chem 278:20514-25 (2003)
DOI:10.1074/jbc.M302149200

Sequence

[mmu:212503]

Other DBs

ExplorEnz - The Enzyme Database:

1.5.3.13

IUBMB Enzyme Nomenclature:

1.5.3.13

ExPASy - ENZYME nomenclature database:

1.5.3.13

BRENDA, the Enzyme Database:

1.5.3.13

DBGET integrated database retrieval system