KEGG ENZYME: 1.7.1.14

ENZYME: 1.7.1.14

Entry

EC 1.7.1.14                 Enzyme

Name

nitric oxide reductase [NAD(P)+, nitrous oxide-forming];
fungal nitric oxide reductase;
cytochrome P450nor;
NOR (ambiguous)

Class

Oxidoreductases;
Acting on other nitrogenous compounds as donors;
With NAD+ or NADP+ as acceptor

Sysname

nitrous oxide:NAD(P) oxidoreductase

Reaction(IUBMB)

N2O + NAD(P)+ + H2O = 2 NO + NAD(P)H + H+ [RN:R02492 R09446]

Reaction(KEGG)

R02492 R09446;
(other) R09808 R09809

Substrate

N2O [CPD:C00887];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]

Product

NO [CPD:C00533];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]

Comment

A heme-thiolate protein (P-450). The enzyme from Fusarium oxysporum utilizes only NADH, but the isozyme from Trichosporon cutaneum utilizes both NADH and NADPH. The electron transfer from NAD(P)H to heme occurs directly, not requiring flavin or other redox cofactors.

History

EC 1.7.1.14 created 2011

Pathway

ec00910

Nitrogen metabolism

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K15877

fungal nitric oxide reductase

Genes

CRE:	CHLRE_01g007950v5

CVR:	CHLNCDRAFT_51513

NCR:

NCU06137

NTE:	NEUTE1DRAFT117740(NEUTE1DRAFT_117740)

SMP:	SMAC_06378

PAN:	PODANSg1411

PBEL:

QC761_512910

PPSD:

QC762_512910

PPSP:

QC763_512910

PPSA:

QC764_512910

TTT:	THITE_2117644

FGR:	FGSG_11585

FVR:	FVEG_10773

FOX:	FOXG_12350

NHE:	NECHADRAFT_41997 NECHADRAFT_89386 NECHADRAFT_97205

FFC:	NCS54_00765400 NCS54_01319500 NCS54_01471300

FKR:	NCS57_00805400 NCS57_01306900

FMU:	J7337_013364

MAW:	MAC_00270 MAC_05732

MAJ:	MAA_08207 MAA_08689

PLJ:	VFPFJ_05955

PTKZ:

JDV02_000549

CFJ:	CFIO01_11821

CLUP:

CLUP02_12747

SAPO:

SAPIO_CDS4621

SSL:	SS1G_00406

MBE:

MBM_04680

PSCO:

LY89DRAFT_614822

GLZ:	GLAREA_09633

AOR:	AO090166000070

ANG:	An07g08930

AFV:	AFLA_011427

URE:	UREG_06342

PBL:	PAAG_02515

PBN:	PADG_05660

ABE:

ARB_00546

TVE:

TRV_00378

AJE:	HCAG_02570

BGH:	BDBG_08235

BSC:	COCSADRAFT_355241

TASA:

A1Q1_03733

CCAC:

CcaHIS019_0312330(CcaverHIS019_0312330)

» show all

Reference

1 [PMID:2040619]

Authors

Shoun H, Tanimoto T

Title

Denitrification by the fungus Fusarium oxysporum and involvement of cytochrome P-450 in the respiratory nitrite reduction.

Journal

J Biol Chem 266:11078-82 (1991)

Sequence

[ag:AAA33337]

Reference

2 [PMID:7829493]

Authors

Shiro Y, Fujii M, Iizuka T, Adachi S, Tsukamoto K, Nakahara K, Shoun H

Title

Spectroscopic and kinetic studies on reaction of cytochrome P450nor with nitric oxide. Implication for its nitric oxide reduction mechanism.

Journal

J Biol Chem 270:1617-23 (1995)
DOI:10.1074/jbc.270.4.1617

Sequence

[ag:AAA33337]

Reference

3 [PMID:12105197]

Authors

Zhang L, Kudo T, Takaya N, Shoun H

Title

The B' helix determines cytochrome P450nor specificity for the electron donors NADH and NADPH.

Journal

J Biol Chem 277:33842-7 (2002)
DOI:10.1074/jbc.M203923200

Sequence

[ag:AAA33337]

Reference

4 [PMID:15313618]

Authors

Oshima R, Fushinobu S, Su F, Zhang L, Takaya N, Shoun H

Title

Structural evidence for direct hydride transfer from NADH to cytochrome P450nor.

Journal

J Mol Biol 342:207-17 (2004)
DOI:10.1016/j.jmb.2004.07.009

Sequence

[ag:AAA33337]

Other DBs

ExplorEnz - The Enzyme Database:

1.7.1.14

IUBMB Enzyme Nomenclature:

1.7.1.14

ExPASy - ENZYME nomenclature database:

1.7.1.14

BRENDA, the Enzyme Database:

1.7.1.14

DBGET integrated database retrieval system