KEGG ENZYME: 2.1.1.247

ENZYME: 2.1.1.247

Entry

EC 2.1.1.247                Enzyme

Name

[methyl-Co(III) methylamine-specific corrinoid protein]---coenzyme M methyltransferase;
methyltransferase 2 (ambiguous);
MT2 (ambiguous);
MT2-A;
mtbA (gene name);
[methyl-Co(III) methylamine-specific corrinoid protein]:coenzyme M methyltransferase

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

methylated monomethylamine-specific corrinoid protein:CoM methyltransferase

Reaction(IUBMB)

a [methyl-Co(III) methylamine-specific corrinoid protein] + CoM = methyl-CoM + a [Co(I) methylamine-specific corrinoid protein] [RN:R10000]

Reaction(KEGG)

R10000;
(other) R09124 R09998 R09999

Substrate

[methyl-Co(III) methylamine-specific corrinoid protein];
CoM [CPD:C03576]

Product

methyl-CoM [CPD:C03920];
[Co(I) methylamine-specific corrinoid protein]

Comment

Contains zinc [2]. The enzyme, which is involved in methanogenesis from mono-, di-, and trimethylamine, catalyses the transfer of a methyl group bound to the cobalt cofactor of several corrinoid proteins (mono-, di-, and trimethylamine-specific corrinoid proteins, cf. EC 2.1.1.248, methylamine---corrinoid protein Co-methyltransferase, EC 2.1.1.249, dimethylamine---corrinoid protein Co-methyltransferase, and EC 2.1.1.250, trimethylamine---corrinoid protein Co-methyltransferase) to CoM, forming the substrate for EC 2.8.4.1, coenzyme-B sulfoethylthiotransferase, the enzyme that catalyses the final step in methanogenesis.

History

EC 2.1.1.247 created 2012

Pathway

ec00680

Methane metabolism

ec01120

Microbial metabolism in diverse environments

Orthology

K14082

[methyl-Co(III) methylamine-specific corrinoid protein]:coenzyme M methyltransferase

Genes

PALM:

RBG61_09535

BLIQ:

INP51_10930

LCG:	L3BBH23_08030

TTF:

THTE_3705

MCAD:

Pan265_12860(hemE)

ALUS:

STSP2_03206(hemE_2)

VBL:	L21SP4_02246

TAZ:	TREAZ_1866

CPOR:

BED41_14920

MBA:	Mbar_A0841

MBY:	MSBRM_0152

MBW:	MSBRW_0160

MBAR:

MSBR2_2229

MBAK:

MSBR3_2280

MAC:	MA_0146(mtbA)

MMA:	MM_1439(mtbA)

MMAZ:

MmTuc01_1497

MMJ:	MSMAS_3225

MMAC:

MSMAC_3247

MVC:	MSVAZ_3363

MEK:	MSKOL_3324

MLS:	MSLAZ_3206

METM:

MSMTP_0576 MSMTP_3048 MSMTP_3126

MEF:	MSWH1_3073

MEQ:	MSWHS_3284

MSJ:	MSSAC_0128

MSZ:	MSSIH_0114

MSW:	MSSIT_0118

MTHR:

MSTHT_1928

MTHE:

MSTHC_1355

MHOR:

MSHOH_3972

MFZ:	AOB57_008940

MBU:	Mbur_2082(mtbA)

MMET:

MCMEM_0281

MELO:

J7W08_11500

MMH:

Mmah_0279

MHAZ:

BHR79_02125

MPOT:

BKM01_06280

MEV:	Metev_0310

MZH:	Mzhil_1020

MPY:	Mpsy_1673 Mpsy_1705

MZI:	HWN40_00845 HWN40_00915 HWN40_05355

MMAV:

RE476_04180(mtbA) RE476_09510(mtbA) RE476_09945(mtbA) RE476_09985(mtbA)

MSEB:

RE474_00475(mtbA) RE474_06455(mtbA) RE474_06550(mtbA)

MHZ:	Metho_0007 Metho_0037 Metho_0355

MEHF:

MmiHf6_09340(hemE_5)

MEES:

MmiEs2_11940(hemE_4)

MER:	MMINT_09350

» show all

Reference

1 [PMID:7635826]

Authors

Burke SA, Krzycki JA

Title

Involvement of the "A" isozyme of methyltransferase II and the 29-kilodalton corrinoid protein in methanogenesis from monomethylamine.

Journal

J Bacteriol 177:4410-6 (1995)
DOI:10.1128/JB.177.15.4410-4416.1995

Sequence

[mba:Mbar_A0841]

Reference

2 [PMID:8702528]

Authors

LeClerc GM, Grahame DA

Title

Methylcobamide:coenzyme M methyltransferase isozymes from Methanosarcina barkeri. Physicochemical characterization, cloning, sequence analysis, and heterologous gene expression.

Journal

J Biol Chem 271:18725-31 (1996)
DOI:10.1074/jbc.271.31.18725

Sequence

[mba:Mbar_A0841]

Reference

3 [PMID:9006042]

Authors

Ferguson DJ Jr, Krzycki JA

Title

Reconstitution of trimethylamine-dependent coenzyme M methylation with the trimethylamine corrinoid protein and the isozymes of methyltransferase II from Methanosarcina barkeri.

Journal

J Bacteriol 179:846-52 (1997)
DOI:10.1128/JB.179.3.846-852.1997

Sequence

[mba:Mbar_A0841]

Reference

4 [PMID:9642198]

Authors

Burke SA, Lo SL, Krzycki JA

Title

Clustered genes encoding the methyltransferases of methanogenesis from monomethylamine.

Journal

J Bacteriol 180:3432-40 (1998)
DOI:10.1128/JB.180.13.3432-3440.1998

Sequence

[mba:Mbar_A0841]

Reference

5 [PMID:10852929]

Authors

Ferguson DJ Jr, Gorlatova N, Grahame DA, Krzycki JA

Title

Reconstitution of dimethylamine:coenzyme M methyl transfer with a discrete corrinoid protein and two methyltransferases purified from Methanosarcina barkeri.

Journal

J Biol Chem 275:29053-60 (2000)
DOI:10.1074/jbc.M910218199

Sequence

[mba:Mbar_A0841]

Other DBs

ExplorEnz - The Enzyme Database:

2.1.1.247

IUBMB Enzyme Nomenclature:

2.1.1.247

ExPASy - ENZYME nomenclature database:

2.1.1.247

BRENDA, the Enzyme Database:

2.1.1.247

DBGET integrated database retrieval system