KEGG ENZYME: 2.5.1.65

ENZYME: 2.5.1.65

Entry

EC 2.5.1.65                 Enzyme

Name

O-phosphoserine sulfhydrylase;
O-phosphoserine(thiol)-lyase

Class

Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

Sysname

O-phospho-L-serine:hydrogen-sulfide 2-amino-2-carboxyethyltransferase

Reaction(IUBMB)

O-phospho-L-serine + hydrogen sulfide = L-cysteine + phosphate [RN:R07274]

Reaction(KEGG)

R07274;
(other) R00897

Substrate

O-phospho-L-serine [CPD:C01005];
hydrogen sulfide [CPD:C00283]

Product

L-cysteine [CPD:C00097];
phosphate [CPD:C00009]

Comment

A pyridoxal-phosphate protein. The enzyme from Aeropyrum pernix acts on both O-phospho-L-serine and O3-acetyl-L-serine, in contrast with EC 2.5.1.47, cysteine synthase, which acts only on O3-acetyl-L-serine.

History

EC 2.5.1.65 created 2004

Pathway

ec00270

Cysteine and methionine metabolism

ec00920

Sulfur metabolism

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

ec01120

Microbial metabolism in diverse environments

Orthology

K10150

cysteine synthase / O-phosphoserine sulfhydrylase / cystathionine beta-synthase

Genes

APE:	APE_1586(cysK)

ACJ:	ACAM_0997(cysK)

DFD:	Desfe_0630

DMU:	Desmu_0644

TAG:

Tagg_1314

IAG:

Igag_1783

THG:	TCELL_0171

HBU:

Hbut_1585

PFM:	Pyrfu_1108

PDL:	Pyrde_0768

PABI:

PABY_05110

PIS:

Pisl_1761

TPE:

Tpen_1605

THB:	N186_00700

TCB:	TCARB_1222

ASC:

ASAC_0793

ACIA:

SE86_05800

CLG:	Calag_0796

CCAI:

NAS2_0810

» show all

Reference

1 [PMID:12965218]

Authors

Mino K, Ishikawa K.

Title

A novel O-phospho-L-serine sulfhydrylation reaction catalyzed by O-acetylserine sulfhydrylase from Aeropyrum pernix K1.

Journal

FEBS Lett 551:133-8 (2003)
DOI:10.1016/S0014-5793(03)00913-X

Reference

2 [PMID:12644499]

Authors

Mino K, Ishikawa K

Title

Characterization of a novel thermostable O-acetylserine sulfhydrylase from Aeropyrum pernix K1.

Journal

J Bacteriol 185:2277-84 (2003)
DOI:10.1128/JB.185.7.2277-2284.2003

Sequence

[ape:APE_1586]

Reference

3 [PMID:12554945]

Authors

Mino K, Oda Y, Ataka M, Ishikawa K.

Title

Crystallization and preliminary X-ray diffraction analysis of O-acetylserine sulfhydrylase from Aeropyrum pernix K1.

Journal

Acta Crystallogr D Biol Crystallogr 59:338-40 (2003)
DOI:10.1107/S0907444902017900

Other DBs

ExplorEnz - The Enzyme Database:

2.5.1.65

IUBMB Enzyme Nomenclature:

2.5.1.65

ExPASy - ENZYME nomenclature database:

2.5.1.65

BRENDA, the Enzyme Database:

2.5.1.65

CAS:	1071505-11-7

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