Entry
Name
biotinidase;
amidohydrolase biotinidase;
biocytinase;
biotin-amide amidohydrolase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
BRITE hierarchy
Sysname
biocytin amidohydrolase
Reaction(IUBMB)
biocytin + H2O = biotin + L-lysine [RN:
R01077 ]
Reaction(KEGG)
Substrate
Product
Comment
The enzyme, found in many bacterial species as well as animals, liberates biotin from biocytin and short biotinylated peptides, but not from biotinylated proteins. It also has activity on biotin esters and biotin amides.
History
EC 3.5.1.12 created 1961, modified 2023
Pathway
Orthology
Genes
MUN : 110543129 110551270(Btd)
NCAR : 124968278 124968503
PVP : 105288551 111739437(BTD)
CAUA : 113062563 113062564
MAMB : 125275859 125276131
LCO : 104939816 109139331 109139332
NCC : 104944686 104944699(btd)
TBEN : 117477819 117478235
PGEO : 117454682 117455173
GACU : 117562835 117562836
EMAC : 134874759(btd) 134874760
ELY : 117263564 117264124 117264125 117264127 117264131
EFO : 125904495 125905085 125905086 125905087 125905088 125905089 125905092 125905093 125905095
SLUC : 116066869 116066871 118493288(btd)
ECRA : 117956338 117956339(btd)
PFLV : 114567763 114567944 114568312
MSAM : 119906734 119906832 119907789
SCHU : 122864009 122864010
CUD : 121523277 121523334 121523416 121524115 121524298 121524362
ALAT : 119014975 119014987
ONL : 100694007 102082273(btd) 109194532 109197882 109198139 109198141 109200911 109200913 109200914 109204954
OAU : 116318428 116333647 116333669(btd) 116333671
PRET : 103472253 103472511
PFOR : 103134997 103135181
PLAI : 106936832(btd) 106943235
PMEI : 106922082 106922093
ALIM : 106521953 106530461
HHIP : 117770677 117770876 117770877
HSP : 118124941 118124942 118124943
SASA : 106570600 106588721 106588750
STRU : 115173883 115175361
OTW : 112240210(btd) 112264467
OMY : 110489374(btd) 110496519
OGO : 124011966 124012249 124043172
OKE : 118380688(btd) 127909650
CCLU : 121572271 121586635
AANG : 118234546 118234547 118234548 118234549 118234550
LCM : 102358398 102358664(BTD)
CSCU : 111631973 111642029
PTEP : 107449055 107449061
ABRU : 129962872 129963244 129981396 129981814 129981872 129981983
DPOL : 127831737 127869322 127875148
» show all
Taxonomy
Reference
Authors
THOMA RW, PETERSON WH.
Title
The enzymatic degradation of soluble bound biotin.
Journal
J Biol Chem 210:569-79 (1954)
Reference
Authors
KNAPPE J, BRUEMMER W, BIEDERBICK K.
Title
[PURIFICATION AND PROPERTIES OF BIOTINIDASE FROM SWINE KIDNEY AND LACTOBACILLUS CASEI.]
Journal
Biochem Z 338:599-613 (1963)
Reference
Authors
Pispa J, Koivusalo M.
Title
Actinomycin D-sensitive increase in the biotinidase activity in mouse liver and serum after ethionine feeding.
Journal
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.12
ExPASy - ENZYME nomenclature database: 3.5.1.12