KEGG ENZYME: 4.2.1.140

ENZYME: 4.2.1.140

Entry

EC 4.2.1.140                Enzyme

Name

gluconate/galactonate dehydratase;
gluconate dehydratase (ambiguous);
Sso3198 (gene name);
Pto0485 (gene name)

Class

Lyases;
Carbon-oxygen lyases;
Hydro-lyases

Sysname

D-gluconate/D-galactonate hydro-lyase

Reaction(IUBMB)

(1) D-gluconate = 2-dehydro-3-deoxy-D-gluconate + H2O [RN:R01538];
(2) D-galactonate = 2-dehydro-3-deoxy-D-galactonate + H2O [RN:R03033]

Reaction(KEGG)

R01538 R03033

Substrate

D-gluconate [CPD:C00257];
D-galactonate [CPD:C00880]

Product

2-dehydro-3-deoxy-D-gluconate [CPD:C00204];
H2O [CPD:C00001];
2-dehydro-3-deoxy-D-galactonate [CPD:C01216]

Comment

The enzyme is involved in glucose and galactose catabolism via the nonphosphorylative variant of the Entner-Doudoroff pathway in Picrophilus torridus [3] and via the branched variant of the Entner-Doudoroff pathway in Sulfolobus solfataricus [1,2]. In vitro it utilizes D-gluconate with 6-10 fold higher catalytic efficiency than D-galactonate [1,3]. It requires Mg2+ for activity [1,2]. cf. EC 4.2.1.6, galactonate dehydratase, and EC 4.2.1.39, gluconate dehydratase.

History

EC 4.2.1.140 created 2013

Pathway

ec00030

Pentose phosphate pathway

ec00052

Galactose metabolism

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K05308

gluconate/galactonate dehydratase

Genes

MTAR:

DF168_00720 DF168_00742

HAL:	VNG_0442G(rspA)

HSL:	OE_1664R(gnaD)

HANR:

LJ422_01860

HHB:	Hhub_1548(gnaD1)

HAGS:

JT689_03140

HABO:

JRZ79_01640

HNO:	LT974_02950 LT974_08915

HLU:	LT972_10545

HAHS:

HSRCO_2771(rspA5)

SALR:

FQU85_02870

HALR:

EFA46_006425 EFA46_011035

HJE:	HacjB3_02665 HacjB3_13980 HacjB3_16911

HDO:	MUK72_10685

HALX:

M0R89_12595

HAXZ:

M0R88_13190

HAYC:

NGM10_04450

HAXY:

NGM07_17265 NGM07_22980

SALI:

L593_03520 L593_07320

HARA:

AArcS_2287(rspA4)

HMA:	rrnAC0575(dgoA4)

HHI:

HAH_1276

HHN:	HISP_06530

HAB:	SG26_14175

HTA:	BVU17_06220

HALJ:

G9465_00310 G9465_11295 G9465_19980

HSIN:

KDQ40_07110

NMO:	Nmlp_1390(gnaD)

HMU:	Hmuk_0321 Hmuk_2254

HALZ:

E5139_04890 E5139_14415

HALL:

LC1Hm_1568(gnaD) LC1Hm_2851(dgoD4)

HAZP:

GBQ70_04885 GBQ70_14430

HALI:

BV210_12330

HSN:	DV733_15585

HRR:	HZS55_02730 HZS55_16360

HPEL:

HZS54_02460 HZS54_19125

HLT:	I7X12_07425 I7X12_08665 I7X12_10485 I7X12_19535

HARC:

HARCEL1_10530

HAAD:

MW046_07370 MW046_14350

HWA:	HQ_2412A(gnaD)

HWC:	Hqrw_2676(gnaD)

HVO:	HVO_1488(gnaD)

HME:	HFX_1553(gnaD)

HGI:	ABY42_07250

HALE:

G3A49_10595

HLS:	KU306_12510

HLN:	SVXHx_1438(gnad)

HBO:	Hbor_17420

HAJ:	DU500_05985

HAER:

DU502_13920

HRA:	EI982_16340

HLM:	DV707_09645

HALM:

FCF25_14340

HLA:	Hlac_1300 Hlac_2579

HALP:

DOS48_06235

HALB:

EKH57_01910

HEZZ:

EO776_08250

HALQ:

Hrr1229_007680 Hrr1229_014835

HSS:	J7656_05055 J7656_08185

HSAI:

HPS36_10285 HPS36_13505

HAZZ:

KI388_05945

SRUB:

C2R22_00660 C2R22_09090

HAE:	halTADL_0374

HALN:

B4589_014190

HALG:

HUG10_06105 HUG10_06895 HUG10_06975 HUG10_07030 HUG10_07280 HUG10_11455 HUG10_16095

HALU:

HUG12_08375 HUG12_08435 HUG12_08585 HUG12_18715

HMP:	K6T50_02880

HRE:	K6T36_02700

HRM:	K6T25_11995

HACB:

Hbl1158_11715

HDF:	AArcSl_1039(gnaD) AArcSl_2834(gnaD2)

HAH:	Halar_1011 Halar_3730

HTU:	Htur_2654 Htur_4388

HSAL:

JMJ58_15575 JMJ58_23050 JMJ58_23365

HAKZ:

J0X25_01030 J0X25_01075

NMG:	Nmag_0821(gnaD1) Nmag_1145(gnaD2) Nmag_1155(gnaD3) Nmag_3936(gnaD4)

HXA:	Halxa_4119 Halxa_4131

NAT:

NJ7G_1251

NPE:	Natpe_2049 Natpe_2057

NVR:	FEJ81_08260 FEJ81_09005 FEJ81_09055 FEJ81_21985

NPL:	FGF80_03725

NAY:	HYG81_01505 HYG81_02195 HYG81_23400 HYG81_24285

HALY:

HYG82_04600 HYG82_13665

HJT:	DVR14_18340 DVR14_18380 DVR14_22470 DVR14_22510

HLO:	J0X27_05005

NGE:	Natgr_0070 Natgr_2179

HRU:	Halru_1854

SAWL:

NGM29_12820 NGM29_14000

HALV:

NGM15_05270

NOU:	Natoc_2547

HLR:	HALLA_00750 HALLA_06625

HLC:	CHINAEXTREME08390 CHINAEXTREME10075 CHINAEXTREME10125

NAJ:	B1756_04600

NAG:	AArcMg_3063

NAN:	AArc1_0639

NARA:

QQ977_13995

NBG:	DV706_11035

NAS:	GCU68_01070 GCU68_01085 GCU68_19305

HDA:	BB347_08605 BB347_14430 BB347_14735

TAC:

Ta0085

TVO:	TVG0179275(TVG0179275)

PTO:

PTO0485

FAC:	FACI_IFERC01G0930

FAI:

FAD_1816

CDIV:

CPM_1948

STO:	STK_23660(gnaD) STK_25720(gnaD)

SOH:	D1869_12965 D1869_13875

SSO:	SSO2665 SSO3198

SOL:	Ssol_0475 Ssol_0935

SSOA:

SULA_0466 SULA_0968

SSOL:

SULB_0468 SULB_0970

SSOF:

SULC_0466 SULC_0969

SSHI:

J5U23_00300 J5U23_00831

SCAS:

SACC_23250 SACC_31330

SAI:

Saci_0885

SACN:

SacN8_04295

SACR:

SacRon12I_04285

SACS:

SUSAZ_04035

SIS:	LS215_2325 LS215_2810

SIA:	M1425_2162 M1425_2649

SIM:	M1627_2242 M1627_2703

SID:	M164_2165 M164_2634

SIY:	YG5714_2288 YG5714_2823

SIN:	YN1551_0627 YN1551_3011

SII:	LD85_2428 LD85_2966

SIH:	SiH_2107 SiH_2603

SIR:	SiRe_2039 SiRe_2542

SIC:	SiL_2014 SiL_2491

SULA:

BFU36_06085 BFU36_09035

SULE:

GFS03_00750 GFS03_11775

SULO:

GFS33_03585

SULL:

EWF20_14025

MSE:

Msed_1303

MEMJ:

MJ1HA_1511

MCN:

Mcup_0900

MHK:	DFR87_11980

MPRU:

DFR88_11805

MTEN:

GWK48_00490

MJN:	MjAS7_2371

AHO:

Ahos_0261

AMAN:

B6F84_04915

ABRI:

DFR85_14595

ASUL:

DFR86_04375

AAMB:

D1866_07685

ACIH:

HS5_15440

SACD:

HS1genome_0402

SAZO:

D1868_08675

CSTY:

KN1_06730

STEP:

IC006_2132

SMET:

RQ359_002106

SAHS:

HS7_10370

PYR:

P186_1932

CMA:	Cmaq_0074 Cmaq_0154

TTN:	TTX_1156(gad)

TUZ:	TUZN_0958 TUZN_0966

VDI:	Vdis_1648 Vdis_2509

VMO:	VMUT_0856 VMUT_2313

VSO:	Vsou_02030

MIY:	Micr_00459

» show all

Reference

1 [PMID:15474024]

Authors

Lamble HJ, Milburn CC, Taylor GL, Hough DW, Danson MJ

Title

Gluconate dehydratase from the promiscuous Entner-Doudoroff pathway in Sulfolobus solfataricus.

Journal

FEBS Lett 576:133-6 (2004)
DOI:10.1016/j.febslet.2004.08.074

Sequence

[sso:SSO3198]

Reference

2 [PMID:15869466]

Authors

Ahmed H, Ettema TJ, Tjaden B, Geerling AC, van der Oost J, Siebers B

Title

The semi-phosphorylative Entner-Doudoroff pathway in hyperthermophilic archaea: a re-evaluation.

Journal

Biochem J 390:529-40 (2005)
DOI:10.1042/BJ20041711

Sequence

[sso:SSO3198]

Reference

3 [PMID:20023024]

Authors

Reher M, Fuhrer T, Bott M, Schonheit P

Title

The nonphosphorylative Entner-Doudoroff pathway in the thermoacidophilic euryarchaeon Picrophilus torridus involves a novel 2-keto-3-deoxygluconate- specific aldolase.

Journal

J Bacteriol 192:964-74 (2010)
DOI:10.1128/JB.01281-09

Sequence

[pto:PTO0485]

Other DBs

ExplorEnz - The Enzyme Database:

4.2.1.140

IUBMB Enzyme Nomenclature:

4.2.1.140

ExPASy - ENZYME nomenclature database:

4.2.1.140

BRENDA, the Enzyme Database:

4.2.1.140

DBGET integrated database retrieval system