Ehmt2 euchromatic histone lysine N-methyltransferase 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Ehmt2provided by MGI
Official Full Name: euchromatic histone lysine N-methyltransferase 2provided by MGI
Primary source: MGI:MGI:2148922
See related: Ensembl:ENSMUSG00000013787 AllianceGenome:MGI:2148922
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: G9a; Bat8; NG36; KMT1C; D17Ertd710e
Summary: Enables several functions, including C2H2 zinc finger domain binding activity; histone-lysine N-methyltransferase activity; and promoter-specific chromatin binding activity. Involved in DNA methylation; histone methylation; and regulation of DNA replication. Acts upstream of or within several processes, including macromolecule methylation; negative regulation of autophagosome assembly; and response to cocaine. Located in nucleus. Is expressed in several structures, including alimentary system; limb; nervous system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in idiopathic pulmonary fibrosis. Orthologous to human EHMT2 (euchromatic histone lysine methyltransferase 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in ovary adult (RPKM 86.7), testis adult (RPKM 83.8) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 17 B1; 17 18.45 cM

Exon count:: 28

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	17	NC_000083.7 (35117427..35133049)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	17	NC_000083.6 (34898463..34914073)

Chromosome 17 - NC_000083.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Epigenetic modifier G9a is involved in regulation of mouse tongue development.
Title: Epigenetic modifier G9a is involved in regulation of mouse tongue development.
G9a Inhibition Promotes Neuroprotection through GMFB Regulation in Alzheimer's Disease.
Title: G9a Inhibition Promotes Neuroprotection through GMFB Regulation in Alzheimer's Disease.
Loss of G9a does not phenocopy the requirement for Prdm12 in the development of the nociceptive neuron lineage.
Title: Loss of G9a does not phenocopy the requirement for Prdm12 in the development of the nociceptive neuron lineage.
G9a and Sirtuin6 epigenetically modulate host cholesterol accumulation to facilitate mycobacterial survival.
Title: G9a and Sirtuin6 epigenetically modulate host cholesterol accumulation to facilitate mycobacterial survival.
Depletion of G9A attenuates imiquimod-induced psoriatic dermatitis via targeting EDAR-NF-kappaB signaling in keratinocyte.
Title: Depletion of G9A attenuates imiquimod-induced psoriatic dermatitis via targeting EDAR-NF-κB signaling in keratinocyte.
G9a promotes immune suppression by targeting the Fbxw7/Notch pathway in glioma stem cells.
Title: G9a promotes immune suppression by targeting the Fbxw7/Notch pathway in glioma stem cells.
Transcriptional coactivation by EHMT2 restricts glucocorticoid-induced insulin resistance in a study with male mice.
Title: Transcriptional coactivation by EHMT2 restricts glucocorticoid-induced insulin resistance in a study with male mice.
G9a Modulates Lipid Metabolism in CD4 T Cells to Regulate Intestinal Inflammation.
Title: G9a Modulates Lipid Metabolism in CD4 T Cells to Regulate Intestinal Inflammation.
EHMT2 methyltransferase governs cell identity in the lung and is required for KRAS (G12D) tumor development and propagation.
Title: EHMT2 methyltransferase governs cell identity in the lung and is required for KRAS (G12D) tumor development and propagation.
Cognitive Decline and BPSD Are Concomitant with Autophagic and Synaptic Deficits Associated with G9a Alterations in Aged SAMP8 Mice.
Title: Cognitive Decline and BPSD Are Concomitant with Autophagic and Synaptic Deficits Associated with G9a Alterations in Aged SAMP8 Mice.

Submit: New GeneRIF Correction See all GeneRIFs (107)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1) 1 citation
Targeted (12) 1 citation
Endonuclease-mediated (4)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D17Ertd710e (e-PCR), detects polymorphism
- UniSTS:167224

NoName (e-PCR)
- UniSTS:237636

Ehmt2 (e-PCR), detects polymorphism
- UniSTS:547694

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables C2H2 zinc finger domain binding	IPI Inferred from Physical Interaction more info	PubMed
enables C2H2 zinc finger domain binding	ISO Inferred from Sequence Orthology more info
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables histone H3K27 methyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables histone H3K27 methyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables histone H3K56 methyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables histone H3K56 methyltransferase activity	ISO Inferred from Sequence Orthology more info
enables histone H3K9 methyltransferase activity	EXP Inferred from Experiment more info	PubMed
enables histone H3K9 methyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables histone H3K9 methyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables histone H3K9 methyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables histone H3K9 monomethyltransferase activity	IEA Inferred from Electronic Annotation more info
enables histone methyltransferase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables methyltransferase activity	IEA Inferred from Electronic Annotation more info
enables p53 binding	ISO Inferred from Sequence Orthology more info
enables promoter-specific chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables promoter-specific chromatin binding	ISO Inferred from Sequence Orthology more info
contributes_to protein binding	IPI Inferred from Physical Interaction more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-lysine N-methyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein-lysine N-methyltransferase activity	ISO Inferred from Sequence Orthology more info	PubMed
enables transcription corepressor binding	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA methylation-dependent heterochromatin formation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within behavioral response to cocaine	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cellular response to cocaine	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within cellular response to starvation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within chromatin organization	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within fertilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in long-term memory	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within methylation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within negative regulation of autophagosome assembly	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of gene expression via chromosomal CpG island methylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info	PubMed
acts_upstream_of_or_within neuron fate specification	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within oocyte development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within organ growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in peptidyl-lysine dimethylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in peptidyl-lysine dimethylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within phenotypic switching	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein methylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA replication	ISO Inferred from Sequence Orthology more info
involved_in regulation of protein modification process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within spermatid development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within synaptonemal complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of chromatin	IBA Inferred from Biological aspect of Ancestor more info
part_of chromatin	ISO Inferred from Sequence Orthology more info	PubMed
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in nuclear speck	ISO Inferred from Sequence Orthology more info
NOT located_in nucleolus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	TAS Traceable Author Statement more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info	PubMed

General protein information

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Preferred Names: histone-lysine N-methyltransferase EHMT2

Names: H3-K9-HMTase 3; HLA-B associated transcript 8; histone H3-K9 methyltransferase 3; histone-lysine N-methyltransferase, H3 lysine-9 specific 3; protein G9a

NP_001273502.1

EC 2.1.1.367

NP_001273504.1

EC 2.1.1.367

NP_001390796.1

EC 2.1.1.367

NP_001390798.1

EC 2.1.1.367

NP_001390799.1

EC 2.1.1.367

NP_665829.1

EC 2.1.1.367

NP_671493.1

EC 2.1.1.367

XP_030105308.1

EC 2.1.1.367

XP_036016153.1

EC 2.1.1.367

XP_036016154.1

EC 2.1.1.367

XP_036016155.1

EC 2.1.1.367

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001286573.2 → NP_001273502.1 histone-lysine N-methyltransferase EHMT2 isoform c

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks an in-frame internal coding exon, compared to variant 1. The resulting isoform (c) lacks an internal segment, compared to isoform a.

Source sequence(s)

CT025759

Consensus CDS

CCDS70798.1

UniProtKB/TrEMBL

A2CG76, Q3U4G9

Related

ENSMUSP00000094955.4, ENSMUST00000097342.10

Conserved Domains (9) summary

smart00468
Location:942 → 1041

PreSET; N-terminal to some SET domains

smart00317
Location:1057 → 1179

SET; SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain

COG2940
Location:956 → 1197

SET; SET domain-containing protein (function unknown) [General function prediction only]

cd00204
Location:705 → 824

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam12796
Location:741 → 834

Ank_2; Ankyrin repeats (3 copies)

sd00045
Location:804 → 834

ANK; ANK repeat [structural motif]

cl02529
Location:673 → 724

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

cl09938
Location:46 → 226

cond_enzymes; Condensing enzymes; Family of enzymes that catalyze a (decarboxylating or non-decarboxylating) Claisen-like condensation reaction. Members are share strong structural similarity, and are involved in the synthesis and degradation of fatty acids, and the ...

cl18951
Location:573 → 608

Amidase; Amidase
NM_001286575.2 → NP_001273504.1 histone-lysine N-methyltransferase EHMT2 isoform d

See identical proteins and their annotated locations for NP_001273504.1

Status: VALIDATED

Description

Transcript Variant: This variant (4) has an alternate 5' terminal exon, which includes the 5' coding sequence, compared to variant 1. The resulting isoform (d) has a shorter and distinct N-terminus, compared to isoform a.

Source sequence(s)

CT025759

Consensus CDS

CCDS70797.1

UniProtKB/TrEMBL

Q3U4G9

Related

ENSMUSP00000109667.3, ENSMUST00000114033.9

Conserved Domains (7) summary

smart00468
Location:919 → 1018

PreSET; N-terminal to some SET domains

smart00317
Location:1034 → 1156

SET; SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain

cd00204
Location:682 → 801

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam12796
Location:718 → 811

Ank_2; Ankyrin repeats (3 copies)

sd00045
Location:781 → 811

ANK; ANK repeat [structural motif]

cl02529
Location:650 → 701

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

cl18951
Location:550 → 585

Amidase; Amidase
NM_001403867.1 → NP_001390796.1 histone-lysine N-methyltransferase EHMT2 isoform e

Status: VALIDATED

Source sequence(s)

CT025759
NM_001403869.1 → NP_001390798.1 histone-lysine N-methyltransferase EHMT2 isoform f

Status: VALIDATED

Source sequence(s)

CT025759
NM_001403870.1 → NP_001390799.1 histone-lysine N-methyltransferase EHMT2 isoform g

Status: VALIDATED

Source sequence(s)

CT025759
NM_145830.3 → NP_665829.1 histone-lysine N-methyltransferase EHMT2 isoform a

See identical proteins and their annotated locations for NP_665829.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longest isoform (a, also known as G9a long).

Source sequence(s)

CT025759

Consensus CDS

CCDS28667.1

UniProtKB/Swiss-Prot

A2CG75, Q6PE08, Q8K4R6, Q8K4R7, Q9Z148, Q9Z149

UniProtKB/TrEMBL

Q3U4G9

Related

ENSMUSP00000013931.6, ENSMUST00000013931.12

Conserved Domains (8) summary

smart00468
Location:976 → 1075

PreSET; N-terminal to some SET domains

smart00317
Location:1091 → 1213

SET; SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain

COG2940
Location:990 → 1231

SET; SET domain-containing protein (function unknown) [General function prediction only]

cd00204
Location:739 → 858

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam12796
Location:775 → 868

Ank_2; Ankyrin repeats (3 copies)

sd00045
Location:838 → 868

ANK; ANK repeat [structural motif]

cl02529
Location:707 → 758

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

cl18951
Location:607 → 642

Amidase; Amidase
NM_147151.3 → NP_671493.1 histone-lysine N-methyltransferase EHMT2 isoform b

See identical proteins and their annotated locations for NP_671493.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) has an alternate 5' terminal exon which includes the 5' coding sequence, and lacks an in-frame internal coding exon, compared to variant 1. The resulting isoform (b, also known as G9a short) has a shorter and distinct N-terminus and lacks an internal segment, compared to isoform a.

Source sequence(s)

CT025759

Consensus CDS

CCDS28666.1

UniProtKB/TrEMBL

Q3U4G9

Related

ENSMUSP00000077208.7, ENSMUST00000078061.13

Conserved Domains (7) summary

smart00468
Location:885 → 984

PreSET; N-terminal to some SET domains

smart00317
Location:1000 → 1122

SET; SET (Su(var)3-9, Enhancer-of-zeste, Trithorax) domain

cd00204
Location:648 → 767

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam12796
Location:684 → 777

Ank_2; Ankyrin repeats (3 copies)

sd00045
Location:747 → 777

ANK; ANK repeat [structural motif]

cl02529
Location:616 → 667

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

cl18951
Location:516 → 551

Amidase; Amidase

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000083.7 Reference GRCm39 C57BL/6J

Range

35117427..35133049

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030249448.2 → XP_030105308.1 histone-lysine N-methyltransferase EHMT2 isoform X4

UniProtKB/TrEMBL

Q3U4G9

Conserved Domains (5) summary

PHA03247
Location:23 → 201

PHA03247; large tegument protein UL36; Provisional

COG0666
Location:628 → 793

ANKYR; Ankyrin repeat [Signal transduction mechanisms]

sd00045
Location:757 → 787

ANK; ANK repeat [structural motif]

pfam12796
Location:761 → 853

Ank_2; Ankyrin repeats (3 copies)

cd10533
Location:920 → 1158

SET_EHMT2; SET domain (including pre-SET and post-SET domains) found in euchromatic histone-lysine N-methyltransferase 2 (EHMT2) and similar proteins
XM_036160261.1 → XP_036016154.1 histone-lysine N-methyltransferase EHMT2 isoform X5

Conserved Domains (5) summary

sd00045
Location:585 → 615

ANK; ANK repeat [structural motif]

cd10533
Location:748 → 986

SET_EHMT2; SET domain (including pre-SET and post-SET domains) found in euchromatic histone-lysine N-methyltransferase 2 (EHMT2) and similar proteins

pfam12796
Location:522 → 615

Ank_2; Ankyrin repeats (3 copies)

cd20905
Location:210 → 339

EHMT_ZBD; Zinc-binding domain of euchromatic histone lysine methyltransferases EHMT1 and EHTM2

cl18951
Location:344 → 379

Amidase
XM_036160260.1 → XP_036016153.1 histone-lysine N-methyltransferase EHMT2 isoform X5

Conserved Domains (5) summary

sd00045
Location:585 → 615

ANK; ANK repeat [structural motif]

cd10533
Location:748 → 986

SET_EHMT2; SET domain (including pre-SET and post-SET domains) found in euchromatic histone-lysine N-methyltransferase 2 (EHMT2) and similar proteins

pfam12796
Location:522 → 615

Ank_2; Ankyrin repeats (3 copies)

cd20905
Location:210 → 339

EHMT_ZBD; Zinc-binding domain of euchromatic histone lysine methyltransferases EHMT1 and EHTM2

cl18951
Location:344 → 379

Amidase
XM_036160262.1 → XP_036016155.1 histone-lysine N-methyltransferase EHMT2 isoform X6

Conserved Domains (6) summary

COG0666
Location:419 → 611

ANKYR; Ankyrin repeat [Signal transduction mechanisms]

sd00045
Location:575 → 605

ANK; ANK repeat [structural motif]

cd10533
Location:738 → 976

SET_EHMT2; SET domain (including pre-SET and post-SET domains) found in euchromatic histone-lysine N-methyltransferase 2 (EHMT2) and similar proteins

pfam12796
Location:512 → 605

Ank_2; Ankyrin repeats (3 copies)

cd20905
Location:210 → 339

EHMT_ZBD; Zinc-binding domain of euchromatic histone lysine methyltransferases EHMT1 and EHTM2

cl18951
Location:344 → 379

Amidase