Slc8a3 solute carrier family 8 (sodium/calcium exchanger), member 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Slc8a3provided by MGI
Official Full Name: solute carrier family 8 (sodium/calcium exchanger), member 3provided by MGI
Primary source: MGI:MGI:107976
See related: Ensembl:ENSMUSG00000079055 AllianceGenome:MGI:107976
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Ncx3
Summary: Enables calcium:sodium antiporter activity and ion antiporter activity involved in regulation of postsynaptic membrane potential. Involved in several processes, including inorganic cation transmembrane transport; learning or memory; and nervous system development. Acts upstream of or within several processes, including hematopoietic progenitor cell differentiation; modulation of excitatory postsynaptic potential; and negative regulation of protein serine/threonine kinase activity. Located in several cellular components, including mitochondrial outer membrane; postsynaptic density; and sarcolemma. Is integral component of plasma membrane. Is expressed in several structures, including bone; brain; foregut; genitourinary system; and trachea. Orthologous to human SLC8A3 (solute carrier family 8 member A3). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in cerebellum adult (RPKM 2.9), frontal lobe adult (RPKM 2.1) and 8 other tissues See more
Orthologs: human all
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Genomic context

Location:: 12 D1; 12 37.44 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	12	NC_000078.7 (81244686..81380506, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	12	NC_000078.6 (81197915..81333732, complement)

Chromosome 12 - NC_000078.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Sodium-calcium exchanger-3 regulates pain ""wind-up"": From human psychophysics to spinal mechanisms.
Title: Sodium-calcium exchanger-3 regulates pain "wind-up": From human psychophysics to spinal mechanisms.
Ncx3-Induced Mitochondrial Dysfunction in Midbrain Leads to Neuroinflammation in Striatum of A53t-alpha-Synuclein Transgenic Old Mice.
Title: Ncx3-Induced Mitochondrial Dysfunction in Midbrain Leads to Neuroinflammation in Striatum of A53t-α-Synuclein Transgenic Old Mice.
TGF-beta Signaling Regulates SLC8A3 Expression and Prevents Oxidative Stress in Developing Midbrain Dopaminergic and Dorsal Raphe Serotonergic Neurons.
Title: TGF-β Signaling Regulates SLC8A3 Expression and Prevents Oxidative Stress in Developing Midbrain Dopaminergic and Dorsal Raphe Serotonergic Neurons.
NCX1 was exclusively co-expressed in IBA-1-positive microglial cells in the striatum, whereas NCX3 was solely co-expressed in tyrosine hydroxylase-positive neurons in substantia nigra.
Title: NCX1 and NCX3 as potential factors contributing to neurodegeneration and neuroinflammation in the A53T transgenic mouse model of Parkinson's Disease.
preconditioning prevented NCX3 downregulation in SOD1 G93A mice spinal cord, leading to an increased number of motor neurons associated to a reduced astrogliosis, and reduced the denervation of neuromuscular junctions observed in SOD1 G93A mice.
Title: Preconditioning, induced by sub-toxic dose of the neurotoxin L-BMAA, delays ALS progression in mice and prevents Na(+)/Ca(2+) exchanger 3 downregulation.
NCX2 and NCX3 protein and mRNA levels are relatively reduced in hippocampal CA1 of APP23 and APP-KI mice. Likewise, NCX2(+/-) or NCX3(+/-) mice exhibited impaired hippocampal LTP and memory-related behaviors. Moreover, relative to controls, calcium/calmodulin-dependent protein kinase II (CaMKII) autophosphorylation significantly decreased in NCX2(+/-) mouse hippocampus but increased in hippocampus of NCX3(+/-) mice.
Title: Reduced expression of Na(+)/Ca(2+) exchangers is associated with cognitive deficits seen in Alzheimer's disease model mice.
This study demonstrate that knocking-out NCX3 impairs oligodendrocyte response and worsens clinical symptoms in EAE without altering the immune T-cell population.
Title: Ncx3 gene ablation impairs oligodendrocyte precursor response and increases susceptibility to experimental autoimmune encephalomyelitis.
Demonstrate that NCX3 genetic deletion protects against ziram-induced neurotoxicity and suggest NCX3 and its downstream molecular pathways as key factors involved in ziram toxicity.
Title: Involvement of the sodium-calcium exchanger 3 (NCX3) in ziram-induced calcium dysregulation and toxicity.
NCX3 regulates mitochondrial calcium handling from the outer mitochondrial membrane through an AKAP121-anchored signaling complex.
Title: NCX3 regulates mitochondrial Ca(2+) handling through the AKAP121-anchored signaling complex and prevents hypoxia-induced neuronal death.
Function and regulation of the Na+-Ca2+ exchanger NCX3 splice variants in brain and skeletal muscle.
Title: Function and regulation of the Na+-Ca2+ exchanger NCX3 splice variants in brain and skeletal muscle.

Submit: New GeneRIF Correction See all GeneRIFs (21)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (4) 1 citation

General gene information

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Markers

Slc8a3 (e-PCR), detects polymorphism
- UniSTS:547137

AW742262 (e-PCR)
- UniSTS:208833

BE110255 (e-PCR)
- UniSTS:245220

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables antiporter activity	IEA Inferred from Electronic Annotation more info
enables calcium:monoatomic cation antiporter activity	IDA Inferred from Direct Assay more info	PubMed
enables calcium:monoatomic cation antiporter activity involved in regulation of postsynaptic cytosolic calcium ion concentration	ISO Inferred from Sequence Orthology more info
enables calcium:sodium antiporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables calcium:sodium antiporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables calcium:sodium antiporter activity	ISO Inferred from Sequence Orthology more info
enables calmodulin binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables monoatomic ion antiporter activity involved in regulation of postsynaptic membrane potential	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in calcium ion export across plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in calcium ion import across plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
involved_in calcium ion import across plasma membrane	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within calcium ion transmembrane transport	IDA Inferred from Direct Assay more info	PubMed
involved_in calcium ion transmembrane transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in calcium ion transmembrane transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within calcium ion transport	IEA Inferred from Electronic Annotation more info
involved_in calcium ion transport	IEA Inferred from Electronic Annotation more info
involved_in calcium ion transport into cytosol	ISO Inferred from Sequence Orthology more info
involved_in cell communication	IEA Inferred from Electronic Annotation more info
involved_in cellular response to cAMP	ISO Inferred from Sequence Orthology more info
involved_in cellular response to hypoxia	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within hematopoietic progenitor cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within intracellular calcium ion homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular calcium ion homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within intracellular calcium ion homeostasis	ISO Inferred from Sequence Orthology more info
involved_in learning	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within learning or memory	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within long-term synaptic potentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in long-term synaptic potentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in memory	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within metal ion transport	IDA Inferred from Direct Assay more info	PubMed
involved_in mitochondrial calcium ion homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrial calcium ion transmembrane transport	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within modulation of excitatory postsynaptic potential	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within monoatomic ion transport	IEA Inferred from Electronic Annotation more info
involved_in myelination	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of protein serine/threonine kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oligodendrocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of skeletal muscle contraction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sodium ion transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in sodium ion transmembrane transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sodium ion transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within synapse organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transmembrane transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in anchoring junction	IEA Inferred from Electronic Annotation more info
is_active_in axon	IBA Inferred from Biological aspect of Ancestor more info
located_in axon	ISO Inferred from Sequence Orthology more info
located_in axon terminus	IMP Inferred from Mutant Phenotype more info	PubMed
located_in cell projection	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in dendrite	ISO Inferred from Sequence Orthology more info
located_in dendritic spine	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in microtubule	ISO Inferred from Sequence Orthology more info
located_in mitochondrial outer membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info
located_in neuromuscular junction	IDA Inferred from Direct Assay more info	PubMed
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IC Inferred by Curator more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in postsynapse	IBA Inferred from Biological aspect of Ancestor more info
located_in postsynaptic density	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in postsynaptic membrane	ISO Inferred from Sequence Orthology more info
is_active_in sarcolemma	IBA Inferred from Biological aspect of Ancestor more info
located_in sarcolemma	IDA Inferred from Direct Assay more info	PubMed
located_in sarcolemma	ISO Inferred from Sequence Orthology more info
located_in synapse	IDA Inferred from Direct Assay more info	PubMed
located_in synapse	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: sodium/calcium exchanger 3

Names: Na(+)/Ca(2+)-exchange protein 3; solute carrier family 8 member 3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001167920.1 → NP_001161392.1 sodium/calcium exchanger 3 isoform 1 precursor

See identical proteins and their annotated locations for NP_001161392.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1.

Source sequence(s)

AK044636, AK139260, BC080862

Consensus CDS

CCDS49103.1

UniProtKB/TrEMBL

Q3UTP5

Related

ENSMUSP00000082334.6, ENSMUST00000085238.13

Conserved Domains (1) summary

TIGR00845
Location:1 → 921

caca; sodium/calcium exchanger 1
NM_001422654.1 → NP_001409583.1 sodium/calcium exchanger 3 isoform 1 precursor

Status: VALIDATED

Source sequence(s)

AC124384
NM_080440.3 → NP_536688.2 sodium/calcium exchanger 3 isoform 2 precursor

Status: VALIDATED

Description

Transcript Variant: This variant (2) contains 2 alternate in-frame coding exons compared to variant 1. This results in a slightly longer isoform (2) containing two protein segments not found in isoform 1.

Source sequence(s)

AF453257, AK044636, AK139260, BC080862

Consensus CDS

CCDS36485.1

UniProtKB/Swiss-Prot

Q7TS90, Q8VHJ8, S4R2P9

UniProtKB/TrEMBL

Q3UTP5

Related

ENSMUSP00000138735.2, ENSMUST00000182208.8

Conserved Domains (1) summary

TIGR00845
Location:1 → 928

caca; sodium/calcium exchanger 1

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000078.7 Reference GRCm39 C57BL/6J

Range

81244686..81380506 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036157165.1 → XP_036013058.1 sodium/calcium exchanger 3 isoform X1

UniProtKB/Swiss-Prot

Q7TS90, Q8VHJ8, S4R2P9

UniProtKB/TrEMBL

Q3UTP5

Conserved Domains (1) summary

TIGR00845
Location:1 → 928

caca; sodium/calcium exchanger 1
XM_006515411.5 → XP_006515474.1 sodium/calcium exchanger 3 isoform X2

See identical proteins and their annotated locations for XP_006515474.1

UniProtKB/TrEMBL

E9PX50, Q3UTP5

Related

ENSMUSP00000063258.8, ENSMUST00000064594.14

Conserved Domains (1) summary

TIGR00845
Location:1 → 927

caca; sodium/calcium exchanger 1
XM_030246527.1 → XP_030102387.1 sodium/calcium exchanger 3 isoform X6

Conserved Domains (1) summary

TIGR00845
Location:10 → 298

caca; sodium/calcium exchanger 1
XM_006515414.4 → XP_006515477.1 sodium/calcium exchanger 3 isoform X5

Conserved Domains (1) summary

TIGR00845
Location:1 → 304

caca; sodium/calcium exchanger 1
XM_036157167.1 → XP_036013060.1 sodium/calcium exchanger 3 isoform X4

UniProtKB/TrEMBL

Q9EPU8

Conserved Domains (1) summary

TIGR00845
Location:1 → 601

caca; sodium/calcium exchanger 1