Braf B-Raf proto-oncogene, serine/threonine kinase [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Brafprovided by RGD
Official Full Name: B-Raf proto-oncogene, serine/threonine kinaseprovided by RGD
Primary source: RGD:619908
See related: Ensembl:ENSRNOG00000010957 AllianceGenome:RGD:619908
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables MAP kinase kinase kinase activity; mitogen-activated protein kinase kinase binding activity; and small GTPase binding activity. Involved in several processes, including cellular response to nerve growth factor stimulus; positive regulation of ERK1 and ERK2 cascade; and response to cAMP. Located in neuron projection; nucleus; and plasma membrane. Used to study bile duct adenoma; cholangiocarcinoma; intrahepatic cholangiocarcinoma; and thyroid gland papillary carcinoma. Human ortholog(s) of this gene implicated in several diseases, including Noonan syndrome 7; carcinoma (multiple); cardiofaciocutaneous syndrome (multiple); central nervous system benign neoplasm (multiple); and melanoma (multiple). Orthologous to human BRAF (B-Raf proto-oncogene, serine/threonine kinase). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Brain (RPKM 22.7), Lung (RPKM 19.6) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 4q23

Exon count:: 26

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	4	NC_086022.1 (69329772..69476931, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	4	NC_051339.1 (68375484..68510652, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	4	NC_005103.4 (67389331..67520549, complement)

Chromosome 4 - NC_086022.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cardiomyocyte BRAF and type 1 RAF inhibitors promote cardiomyocyte and cardiac hypertrophy in mice in vivo.
Title: Cardiomyocyte BRAF and type 1 RAF inhibitors promote cardiomyocyte and cardiac hypertrophy in mice in vivo.
stimulation of B-Raf strongly activated the transcription factor AP-1 which is accompanied by increased c-Jun and c-Fos promoter activities, an upregulation of c-Jun and c-Fos biosynthesis, and elevated transcriptional activation potentials of c-Jun and c-Fos.
Title: Stimulation of B-Raf increases c-Jun and c-Fos expression and upregulates AP-1-regulated gene transcription in insulinoma cells.
The gliomas were immunoreactive to BRAF V600E antibody. These results indicate that Braf mutation is a frequent early event in the development of rat gliomas caused by a single dose of N-ethyl-N-nitrosourea (ENU).
Title: Braf Mutations Initiate the Development of Rat Gliomas Induced by Postnatal Exposure to N-Ethyl-N-Nitrosourea.
Study provides evidence that transcriptional and post-translational modification of B-Raf may contribute to the progression of tuberous sclerosis renal cell carcinoma.
Title: Transcriptional and post-translational modifications of B-Raf in quinol-thioether induced tuberous sclerosis renal cell carcinoma.
B-RAF kinase activity is essential for cardiac hypertrophy and RCN1, its newly identified negative regulator
Title: B-RAF and its novel negative regulator reticulocalbin 1 (RCN1) modulates cardiomyocyte hypertrophy.
BRAF alternative splicing is dispensable for development but required for learning and memory associated with the hippocampus. Exons 8b and 9b are expressed in rodent species.
Title: B-raf alternative splicing is dispensable for development but required for learning and memory associated with the hippocampus in the adult mouse.
B-raf is required for extracellular signal-regulated kinase activation by thyrotropin, and is necessary for thyrotropin-stimulated DNA synthesis.
Title: Protein kinase A and B-Raf mediate extracellular signal-regulated kinase activation by thyrotropin.
selective modulation of c-Raf-1 but not B-Raf activation by RKIP can limit the dynamic range of the MAPK signaling response to growth factors and may play a critical role in growth and development
Title: Raf kinase inhibitory protein regulates Raf-1 but not B-Raf kinase activation.
DYRK1A prolongs the kinetics of ERK activation by interacting with Ras, B-Raf, and MEK1 to facilitate the formation of a Ras/B-Raf/MEK1 multiprotein complex
Title: DYRK1A enhances the mitogen-activated protein kinase cascade in PC12 cells by forming a complex with Ras, B-Raf, and MEK1.
observations establish Rin as a neuronal specific regulator of neurotrophin signaling, required to couple NGF stimulation to sustain activation of p38 MAP kinase and b-Raf signaling cascades required for neuronal development
Title: Rin GTPase couples nerve growth factor signaling to p38 and b-Raf/ERK pathways to promote neuronal differentiation.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables MAP kinase kinase activity	ISO Inferred from Sequence Orthology more info
enables MAP kinase kinase kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables MAP kinase kinase kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables MAP kinase kinase kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables calcium ion binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables mitogen-activated protein kinase kinase binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables protein-containing complex binding	IPI Inferred from Physical Interaction more info	PubMed
enables scaffold protein binding	ISO Inferred from Sequence Orthology more info
enables small GTPase binding	IDA Inferred from Direct Assay more info	PubMed
NOT enables small GTPase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within CD4-positive or CD8-positive, alpha-beta T cell lineage commitment	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within CD4-positive, alpha-beta T cell differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of ERK1 and ERK2 cascade	ISO Inferred from Sequence Orthology more info
involved_in MAPK cascade	IBA Inferred from Biological aspect of Ancestor more info
involved_in MAPK cascade	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within T cell differentiation in thymus	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within T cell receptor signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within alpha-beta T cell differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in cellular response to calcium ion	ISO Inferred from Sequence Orthology more info
involved_in cellular response to nerve growth factor stimulus	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within cellular response to xenobiotic stimulus	ISO Inferred from Sequence Orthology more info
acts_upstream_of endothelial cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in epidermal growth factor receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in establishment of protein localization to membrane	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within face development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within head morphogenesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within long-term synaptic potentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within myeloid progenitor cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of endothelial cell apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of fibroblast migration	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of neuron apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of neuron apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of synaptic vesicle exocytosis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive T cell selection	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of ERK1 and ERK2 cascade	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of ERK1 and ERK2 cascade	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of ERK1 and ERK2 cascade	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of axon regeneration	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of axonogenesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of glucose transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of peptidyl-serine phosphorylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of stress fiber assembly	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of substrate adhesion-dependent cell spreading	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of T cell differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of axon regeneration	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in response to cAMP	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to peptide hormone	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within somatic stem cell population maintenance	ISO Inferred from Sequence Orthology more info
acts_upstream_of stress fiber assembly	ISO Inferred from Sequence Orthology more info
acts_upstream_of substrate adhesion-dependent cell spreading	ISO Inferred from Sequence Orthology more info
acts_upstream_of synaptic vesicle exocytosis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within thymus development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within thyroid gland development	ISO Inferred from Sequence Orthology more info
involved_in trehalose metabolism in response to stress	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within visual learning	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cell body	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
located_in neuron projection	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in presynapse	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: serine/threonine-protein kinase B-raf

Names: v-raf murine sarcoma viral oncogene homolog B; v-raf murine sarcoma viral oncogene homolog B1

NP_001414395.1

EC 2.7.11.1

XP_006236419.3

EC 2.7.11.1

XP_063141466.1

EC 2.7.11.1

XP_063141467.1

EC 2.7.11.1

XP_063141468.1

EC 2.7.11.1

XP_231692.4

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001427466.2 → NP_001414395.1 serine/threonine-protein kinase B-raf

Status: VALIDATED

Source sequence(s)

JAXUCZ010000004

UniProtKB/TrEMBL

A0A8I6ADL5, A6IEW0

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086022.1 Reference GRCr8

Range

69329772..69476931 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063285398.1 → XP_063141468.1 serine/threonine-protein kinase B-raf isoform X5
XM_231692.11 → XP_231692.4 serine/threonine-protein kinase B-raf isoform X3

See identical proteins and their annotated locations for XP_231692.4

UniProtKB/TrEMBL

A0A8I6AFV6, A0A8I6ATH0

Related

ENSRNOP00000096915.1

Conserved Domains (4) summary

cd01816
Location:139 → 210

Raf_RBD; Ubiquitin domain of Raf serine/threonine kinases

cd14062
Location:500 → 752

STKc_Raf; Catalytic domain of the Serine/Threonine Kinases, Raf (Rapidly Accelerated Fibrosarcoma) kinases

pfam00069
Location:495 → 750

Pkinase; Protein kinase domain

pfam00130
Location:218 → 263

C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
XM_006236357.5 → XP_006236419.3 serine/threonine-protein kinase B-raf isoform X2

UniProtKB/TrEMBL

A0A8I6AFV6
XM_063285397.1 → XP_063141467.1 serine/threonine-protein kinase B-raf isoform X4
XM_063285396.1 → XP_063141466.1 serine/threonine-protein kinase B-raf isoform X1