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Pfkfb3 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 [ Mus musculus (house mouse) ]

Gene ID: 170768, updated on 12-Apr-2024

Summary

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Official Symbol
Pfkfb3provided by MGI
Official Full Name
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3provided by MGI
Primary source
MGI:MGI:2181202
See related
Ensembl:ENSMUSG00000026773 AllianceGenome:MGI:2181202
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
iPFK-2; uPFK-2; E330010H22Rik
Summary
Predicted to enable 6-phosphofructo-2-kinase activity and fructose-2,6-bisphosphate 2-phosphatase activity. Predicted to be involved in fructose 2,6-bisphosphate metabolic process. Predicted to be located in nucleoplasm. Predicted to be active in cytosol. Is expressed in several structures, including alimentary system; integumental system; nervous system; sensory organ; and skeleton. Used to study extrinsic cardiomyopathy. Orthologous to human PFKFB3 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Broad expression in subcutaneous fat pad adult (RPKM 57.0), genital fat pad adult (RPKM 44.9) and 22 other tissues See more
Orthologs
human all
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Genomic context

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See Pfkfb3 in Genome Data Viewer
Location:
2 A1; 2 8.73 cM
Exon count:
21
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 2 NC_000068.8 (11476241..11558882, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 2 NC_000068.7 (11471430..11554071, complement)

Chromosome 2 - NC_000068.8Genomic Context describing neighboring genes Neighboring gene glyceraldehyde-3-phosphate dehydrogenase pseudogene Neighboring gene predicted gene, 31990 Neighboring gene STARR-positive B cell enhancer ABC_E5882 Neighboring gene STARR-seq mESC enhancer starr_03667 Neighboring gene predicted gene, 52500 Neighboring gene predicted gene 10851 Neighboring gene STARR-seq mESC enhancer starr_03668 Neighboring gene CapStarr-seq enhancer MGSCv37_chr2:11470208-11470361 Neighboring gene STARR-positive B cell enhancer mm9_chr2:11475523-11475823 Neighboring gene STARR-seq mESC enhancer starr_03673 Neighboring gene STARR-positive B cell enhancer ABC_E5883 Neighboring gene RNA binding motif protein 17 Neighboring gene proteasome (prosome, macropain) 26S subunit, ATPase, 6 pseudogene

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. PFKFB3 downregulation aggravates Angiotensin II-induced podocyte detachment. Huang X, et al. Ren Fail, 2023 Dec. PMID 37427767, Free PMC Article
  2. PFKFB3 mediates tubular cell death in cisplatin nephrotoxicity by activating CDK4. Wen L, et al. Transl Res, 2023 Mar. PMID 36243313, Free PMC Article
  3. Differential role of neuronal glucose and PFKFB3 in memory formation during development. Cruz E, et al. Glia, 2022 Nov. PMID 35916383, Free PMC Article
  4. Gene-dosage effect of Pfkfb3 on monocyte/macrophage biology in atherosclerosis. Guo S, et al. Br J Pharmacol, 2022 Nov. PMID 35834356, Free PMC Article
  5. Suppression of myeloid PFKFB3-driven glycolysis protects mice from choroidal neovascularization. Liu Z, et al. Br J Pharmacol, 2022 Nov. PMID 35830274, Free PMC Article

See all (81) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Context-dependent modification of PFKFB3 in hematopoietic stem cells promotes anaerobic glycolysis and ensures stress hematopoiesis.
    Title: Context-dependent modification of PFKFB3 in hematopoietic stem cells promotes anaerobic glycolysis and ensures stress hematopoiesis.
  2. IL1R2 promotes retinal angiogenesis to participate in retinopathy of prematurity by activating the HIF1alpha/PFKFB3 pathway.
    Title: IL1R2 promotes retinal angiogenesis to participate in retinopathy of prematurity by activating the HIF1α/PFKFB3 pathway.
  3. PFKFB3-Meditated Glycolysis via the Reactive Oxygen Species-Hypoxic Inducible Factor 1alpha Axis Contributes to Inflammation and Proliferation of Staphylococcus aureus in Epithelial Cells.
    Title: PFKFB3-Meditated Glycolysis via the Reactive Oxygen Species-Hypoxic Inducible Factor 1α Axis Contributes to Inflammation and Proliferation of Staphylococcus aureus in Epithelial Cells.
  4. Targeting the SphK1/S1P/PFKFB3 axis suppresses hepatocellular carcinoma progression by disrupting glycolytic energy supply that drives tumor angiogenesis.
    Title: Targeting the SphK1/S1P/PFKFB3 axis suppresses hepatocellular carcinoma progression by disrupting glycolytic energy supply that drives tumor angiogenesis.
  5. Inhibition of PFKFB3 Expression Stimulates Macrophage-Mediated Lymphangiogenesis Post-Acute Myocardial Infarction.
    Title: Inhibition of PFKFB3 Expression Stimulates Macrophage-Mediated Lymphangiogenesis Post-Acute Myocardial Infarction.
  6. Myeloid PFKFB3-mediated glycolysis promotes kidney fibrosis.
    Title: Myeloid PFKFB3-mediated glycolysis promotes kidney fibrosis.
  7. cGAS-STING signaling pathway promotes hypoxia-induced renal fibrosis by regulating PFKFB3-mediated glycolysis.
    Title: cGAS-STING signaling pathway promotes hypoxia-induced renal fibrosis by regulating PFKFB3-mediated glycolysis.
  8. PFKFB3 promotes sepsis-induced acute lung injury by enhancing NET formation by CXCR4[hi] neutrophils.
    Title: PFKFB3 promotes sepsis-induced acute lung injury by enhancing NET formation by CXCR4(hi) neutrophils.
  9. PFKFB3-driven vascular smooth muscle cell glycolysis promotes vascular calcification via the altered FoxO3 and lactate production.
    Title: PFKFB3-driven vascular smooth muscle cell glycolysis promotes vascular calcification via the altered FoxO3 and lactate production.
  10. PFKFB3 downregulation aggravates Angiotensin II-induced podocyte detachment.
    Title: PFKFB3 downregulation aggravates Angiotensin II-induced podocyte detachment.
Submit: New GeneRIF Correction See all GeneRIFs (53)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables 6-phosphofructo-2-kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 6-phosphofructo-2-kinase activity ISO
Inferred from Sequence Orthology
more info
  
enables fructose-2,6-bisphosphate 2-phosphatase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
Process Evidence Code Pubs
involved_in fructose 2,6-bisphosphate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in fructose 2,6-bisphosphate metabolic process ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleoplasm ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
Names
inducible 6-phosphofructo-2-kinase
NP_001171223.1
NP_001171224.1
NP_001171225.1
NP_001171226.1
NP_001171227.1
NP_001171228.1
NP_001171229.1
NP_573495.2
XP_006497430.1
XP_006497432.1
XP_017171443.1
XP_030103781.1
XP_030103790.1
XP_036014832.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001177752.1 → NP_001171223.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 1

    See identical proteins and their annotated locations for NP_001171223.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) lacks 4 exons in the 3' coding region, compared to variant 2. The resulting isoform (1) has a shorter and distinct C-terminus, compared to isoform 2.
    Source sequence(s)
    BB553577, BC052400, BP764506, BY267778, EU034675
    Consensus CDS
    CCDS50498.1
    UniProtKB/TrEMBL
    A2AUP3, A7UAK4, V9GWY6
    Related
    ENSMUSP00000138893.3, ENSMUST00000183869.8
    Conserved Domains (2) summary
    pfam00300
    Location:249 → 432
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:26 → 246
    6PF2K; 6-phosphofructo-2-kinase

  2. NM_001177753.1 → NP_001171224.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 2

    See identical proteins and their annotated locations for NP_001171224.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2).
    Source sequence(s)
    BB553577, BC052400, BP764506, BY267778, EU034676
    Consensus CDS
    CCDS50501.1
    UniProtKB/TrEMBL
    A2AUP4, A7UAK5
    Related
    ENSMUSP00000142079.2, ENSMUST00000191668.6
    Conserved Domains (2) summary
    pfam00300
    Location:249 → 432
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:26 → 246
    6PF2K; 6-phosphofructo-2-kinase

  3. NM_001177754.1 → NP_001171225.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 3

    See identical proteins and their annotated locations for NP_001171225.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) lacks two alternate exons in the 3' coding region, compared to variant 2. The resulting isoform (3) lacks an internal segment and has a shorter and distinct C-terminus, compared to isoform 2.
    Source sequence(s)
    BB553577, BC052400, BP764506, BY267778, EU034677
    Consensus CDS
    CCDS79731.1
    UniProtKB/TrEMBL
    A2AUP3, A2AUP5, A7UAK6
    Related
    ENSMUSP00000050926.8, ENSMUST00000049849.12
    Conserved Domains (2) summary
    pfam00300
    Location:249 → 432
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:26 → 246
    6PF2K; 6-phosphofructo-2-kinase

  4. NM_001177755.1 → NP_001171226.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 4

    See identical proteins and their annotated locations for NP_001171226.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (4) lacks two alternate exons in the 3' coding region, compared to variant 2. The resulting isoform (4) has a shorter and distinct C-terminus, compared to isoform 2.
    Source sequence(s)
    BB553577, BC052400, BP764506, BY267778, EU034678
    Consensus CDS
    CCDS79732.1
    UniProtKB/TrEMBL
    A2AUP4, Q3U3S6
    Related
    ENSMUSP00000137130.3, ENSMUST00000179584.8
    Conserved Domains (2) summary
    pfam00300
    Location:249 → 432
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:26 → 246
    6PF2K; 6-phosphofructo-2-kinase

  5. NM_001177756.1 → NP_001171227.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 5

    See identical proteins and their annotated locations for NP_001171227.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (5) lacks one alternate exon in the 3' coding region, compared to variant 2. The resulting isoform (5) has a shorter and distinct C-terminus, compared to isoform 2.
    Source sequence(s)
    BB553577, BC052400, BP764506, BY267778, EU034679
    Consensus CDS
    CCDS50500.1
    UniProtKB/TrEMBL
    A2AUP4, A7UAK8
    Related
    ENSMUSP00000126305.3, ENSMUST00000170196.9
    Conserved Domains (2) summary
    pfam00300
    Location:249 → 432
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:26 → 246
    6PF2K; 6-phosphofructo-2-kinase

  6. NM_001177757.1 → NP_001171228.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 7

    See identical proteins and their annotated locations for NP_001171228.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (7) lacks an alternate in-frame exon in the 3' coding region, compared to variant 2. The resulting isoform (7) lacks an internal segment, compared to isoform 2.
    Source sequence(s)
    BB553577, BC052400, BP764506, BY267778
    Consensus CDS
    CCDS50499.1
    UniProtKB/TrEMBL
    A2AUP3, Q7TS91
    Related
    ENSMUSP00000028114.7, ENSMUST00000028114.13
    Conserved Domains (2) summary
    pfam00300
    Location:249 → 432
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:26 → 246
    6PF2K; 6-phosphofructo-2-kinase

  7. NM_001177758.1 → NP_001171229.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 8

    Status: VALIDATED

    Description
    Transcript Variant: This variant (8) represents use of an alternate promoter and 5' UTR, uses a distinct translation start codon, and lacks three alternate exons in the 3' coding region, compared to variant 2. The resulting isoform (8) has a distinct N-terminus, lacks an internal segment, and has a shorter and distinct C-terminus, compared to isoform 2.
    Source sequence(s)
    AK143330, AL831794, BC052400, BP764506, EU034678
    Consensus CDS
    CCDS79733.1
    UniProtKB/TrEMBL
    A0A0A6YY64, Q9ESY2
    Related
    ENSMUSP00000142296.2, ENSMUST00000192949.6
    Conserved Domains (2) summary
    pfam00300
    Location:229 → 412
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:4 → 226
    6PF2K; 6-phosphofructo-2-kinase

  8. NM_133232.3 → NP_573495.2  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform 6

    Status: VALIDATED

    Description
    Transcript Variant: This variant (6) lacks three alternate exons in the 3' coding region, compared to variant 2. The resulting isoform (6) lacks an internal segment and has a shorter and distinct C-terminus, compared to isoform 2.
    Source sequence(s)
    BB553577, BC052400, BP764506, BY267778
    Consensus CDS
    CCDS15683.1
    UniProtKB/TrEMBL
    A2AUP1, Q9ESY2
    Related
    ENSMUSP00000110495.3, ENSMUST00000114846.9
    Conserved Domains (2) summary
    pfam00300
    Location:249 → 432
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:26 → 246
    6PF2K; 6-phosphofructo-2-kinase

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000068.8 Reference GRCm39 C57BL/6J

    Range
    11476241..11558882 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_036158939.1 → XP_036014832.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X4

    UniProtKB/TrEMBL
    A2AUP3
    Conserved Domains (2) summary
    pfam00300
    Location:229 → 415
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:4 → 226
    6PF2K; 6-phosphofructo-2-kinase

  2. XM_006497367.1 → XP_006497430.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X2

    UniProtKB/TrEMBL
    A2AUP4
    Conserved Domains (2) summary
    pfam01591
    Location:7 → 226
    6PF2K; 6-phosphofructo-2-kinase
    pfam00300
    Location:229 → 412
    His_Phos_1; Histidine phosphatase superfamily (branch 1)

  3. XM_030247921.2 → XP_030103781.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X1

    UniProtKB/TrEMBL
    A2AUP3
    Conserved Domains (2) summary
    pfam01591
    Location:26 → 246
    6PF2K; 6-phosphofructo-2-kinase
    pfam00300
    Location:249 → 432
    His_Phos_1; Histidine phosphatase superfamily (branch 1)

  4. XM_006497369.4 → XP_006497432.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X5

    See identical proteins and their annotated locations for XP_006497432.1

    UniProtKB/TrEMBL
    A2AUP4
    Conserved Domains (2) summary
    pfam01591
    Location:1 → 207
    6PF2K; 6-phosphofructo-2-kinase
    pfam00300
    Location:210 → 393
    His_Phos_1; Histidine phosphatase superfamily (branch 1)

  5. XM_030247930.1 → XP_030103790.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X3

    UniProtKB/TrEMBL
    A2AUP4
    Conserved Domains (2) summary
    pfam01591
    Location:9 → 228
    6PF2K; 6-phosphofructo-2-kinase
    pfam00300
    Location:231 → 414
    His_Phos_1; Histidine phosphatase superfamily (branch 1)

  6. XM_017315954.3 → XP_017171443.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 isoform X6

    UniProtKB/TrEMBL
    Q8BVM1, Q9ESY2
    Conserved Domains (2) summary
    pfam01591
    Location:9 → 228
    6PF2K; 6-phosphofructo-2-kinase
    pfam00300
    Location:231 → 414
    His_Phos_1; Histidine phosphatase superfamily (branch 1)

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

  1. NM_172976.1: Suppressed sequence

    Description
    NM_172976.1: This RefSeq was permanently suppressed because it is a nonsense-mediated decay (NMD) candidate.
Nucleotide Protein Strain
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