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Asap2 ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 [ Mus musculus (house mouse) ]

Gene ID: 211914, updated on 11-Apr-2024

Summary

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Official Symbol
Asap2provided by MGI
Official Full Name
ArfGAP with SH3 domain, ankyrin repeat and PH domain 2provided by MGI
Primary source
MGI:MGI:2685438
See related
Ensembl:ENSMUSG00000052632 AllianceGenome:MGI:2685438
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
PAP; PAG3; Ddef2; Gm592; Gm1523; 6530401G17Rik
Summary
Predicted to enable GTPase activator activity. Predicted to be involved in positive regulation of GTPase activity. Predicted to be located in Golgi apparatus and plasma membrane. Is expressed in brain and liver. Orthologous to human ASAP2 (ArfGAP with SH3 domain, ankyrin repeat and PH domain 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in CNS E14 (RPKM 10.8), bladder adult (RPKM 10.1) and 27 other tissues See more
Orthologs
human all
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Genomic context

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Location:
12 A1.3; 12 8.29 cM
Exon count:
31
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 12 NC_000078.7 (21161369..21320172)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 12 NC_000078.6 (21111368..21270171)

Chromosome 12 - NC_000078.7Genomic Context describing neighboring genes Neighboring gene RIKEN cDNA 3110053B16 gene Neighboring gene STARR-seq mESC enhancer starr_31716 Neighboring gene STARR-seq mESC enhancer starr_31717 Neighboring gene predicted gene 5977 Neighboring gene STARR-seq mESC enhancer starr_31718 Neighboring gene STARR-seq mESC enhancer starr_31719 Neighboring gene STARR-seq mESC enhancer starr_31721 Neighboring gene STARR-seq mESC enhancer starr_31722 Neighboring gene predicted gene, 40855 Neighboring gene integrin beta 1 binding protein 1 Neighboring gene predicted gene, 25821 Neighboring gene cleavage and polyadenylation specificity factor 3 Neighboring gene a disintegrin and metallopeptidase domain 17 Neighboring gene isoamyl acetate-hydrolyzing esterase 1 homolog

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Crystal structure of the ARF-GAP domain and ankyrin repeats of PYK2-associated protein beta. Mandiyan V, et al. EMBO J, 1999 Dec 15. PMID 10601011, Free PMC Article
  2. PAG3/Papalpha/KIAA0400, a GTPase-activating protein for ADP-ribosylation factor (ARF), regulates ARF6 in Fcgamma receptor-mediated phagocytosis of macrophages. Uchida H, et al. J Exp Med, 2001 Apr 16. PMID 11304556, Free PMC Article
  3. Selenoprotein K regulation of palmitoylation and calpain cleavage of ASAP2 is required for efficient FcγR-mediated phagocytosis. Norton RL, et al. J Leukoc Biol, 2017 Feb. PMID 27601625, Free PMC Article
  4. Quantitative comparison of CrkL-SH3 binding proteins from embryonic murine brain and liver: Implications for developmental signaling and the quantification of protein species variants in bottom-up proteomics. Cheerathodi M, et al. J Proteomics, 2015 Jul 1. PMID 25982384, Free PMC Article
  5. ADP-ribosylation factor-like GTPase 15 enhances insulin-induced AKT phosphorylation in the IR/IRS1/AKT pathway by interacting with ASAP2 and regulating PDPK1 activity. Zhao J, et al. Biochem Biophys Res Commun, 2017 May 13. PMID 28322786

See all (31) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. these findings identify ASAP2 as a new target of Selk-dependent palmitoylation and reveal a new mechanism regulating the efficiency of FcgammaR-mediated phagocytosis.
    Title: Selenoprotein K regulation of palmitoylation and calpain cleavage of ASAP2 is required for efficient FcγR-mediated phagocytosis.
  2. In conclusion, we have identified that ARL15 acts as an insulin-sensitizing effector molecule to upregulate the phosphorylation of members of the canonical IR/IRS1/PDPK1/AKT insulin pathway by interacting with its GAP ASAP2 and activating PDPK1. This research may provide new insights into GTPase-mediated insulin signalling regulation and facilitate the development of new pharmacotherapeutic targets for insulin sensitizati
    Title: ADP-ribosylation factor-like GTPase 15 enhances insulin-induced AKT phosphorylation in the IR/IRS1/AKT pathway by interacting with ASAP2 and regulating PDPK1 activity.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (4) 

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • MGC90837

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables GTPase activator activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in positive regulation of GTPase activity IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in Golgi apparatus IEA
Inferred from Electronic Annotation
more info
  
is_active_in cellular_component ND
No biological Data available
more info
  
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2
Names
development and differentiation enhancing factor 2
paxillin-associated protein with ARF GAP activity 3
pyk2 C-terminus-associated protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001004364.2 → NP_001004364.2  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform b

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) uses an alternate in-frame splice site and lacks two alternate in-frame exons, compared to variant 3. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform c.
    Source sequence(s)
    BC080847, BC096022
    Consensus CDS
    CCDS49036.1
    UniProtKB/TrEMBL
    D3YX85, Q3UH05
    Related
    ENSMUSP00000054631.10, ENSMUST00000050990.10
    Conserved Domains (8) summary
    cd11966
    Location:897 → 952
    SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
    cd00204
    Location:579 → 673
    ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
    cd13251
    Location:297 → 403
    PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
    pfam00169
    Location:306 → 393
    PH; PH domain
    pfam01412
    Location:421 → 538
    ArfGap; Putative GTPase activating protein for Arf
    pfam13857
    Location:607 → 661
    Ank_5; Ankyrin repeats (many copies)
    sd00045
    Location:586 → 618
    ANK; ANK repeat [structural motif]
    cl12013
    Location:34 → 248
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature

  2. NM_001098168.1 → NP_001091637.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform a

    See identical proteins and their annotated locations for NP_001091637.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) uses an alternate in-frame splice site and lacks an alternate in-frame exon in the 3' coding region, compared to variant 3. The resulting isoform (a) has the same N- and C-termini but is shorter compared to isoform c.
    Source sequence(s)
    BC080847, BC096022
    Consensus CDS
    CCDS56832.1
    UniProtKB/Swiss-Prot
    Q501K1, Q66JN2, Q7SIG6
    UniProtKB/TrEMBL
    Q3UH05
    Related
    ENSMUSP00000099098.5, ENSMUST00000101562.11
    Conserved Domains (8) summary
    cd11966
    Location:900 → 955
    SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
    cd00204
    Location:582 → 676
    ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
    cd13251
    Location:300 → 406
    PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
    pfam00169
    Location:309 → 396
    PH; PH domain
    pfam01412
    Location:424 → 541
    ArfGap; Putative GTPase activating protein for Arf
    pfam13857
    Location:610 → 664
    Ank_5; Ankyrin repeats (many copies)
    sd00045
    Location:589 → 621
    ANK; ANK repeat [structural motif]
    cl12013
    Location:34 → 248
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature

  3. NM_001135192.1 → NP_001128664.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform c

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (c).
    Source sequence(s)
    AC156032, AK147622, BC096022, CD351639
    Consensus CDS
    CCDS49037.1
    UniProtKB/TrEMBL
    E9PX52, Q3UH27
    Related
    ENSMUSP00000063217.9, ENSMUST00000064595.15
    Conserved Domains (8) summary
    cd07642
    Location:34 → 248
    BAR_ASAP2; The Bin/Amphiphysin/Rvs (BAR) domain of ArfGAP with SH3 domain, ANK repeat and PH domain containing protein 2
    cd11966
    Location:942 → 997
    SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
    cd00204
    Location:579 → 673
    ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...
    cd13251
    Location:297 → 403
    PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
    pfam00169
    Location:306 → 393
    PH; PH domain
    pfam01412
    Location:421 → 538
    ArfGap; Putative GTPase activating protein for Arf
    pfam13857
    Location:607 → 661
    Ank_5; Ankyrin repeats (many copies)
    sd00045
    Location:586 → 618
    ANK; ANK repeat [structural motif]

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000078.7 Reference GRCm39 C57BL/6J

    Range
    21161369..21320172
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006515047.2 → XP_006515110.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X1

    UniProtKB/TrEMBL
    Q3UH27
    Conserved Domains (6) summary
    cd07642
    Location:34 → 248
    BAR_ASAP2; The Bin/Amphiphysin/Rvs (BAR) domain of ArfGAP with SH3 domain, ANK repeat and PH domain containing protein 2
    cd11966
    Location:945 → 1000
    SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
    cd13251
    Location:300 → 406
    PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
    sd00045
    Location:589 → 621
    ANK; ANK repeat [structural motif]
    cd08849
    Location:422 → 544
    ArfGap_ASAP2; ArfGAP domain of ASAP2 (ArfGAP2 with SH3 domain, ANK repeat and PH domain-containing protein 2)
    pfam12796
    Location:592 → 676
    Ank_2; Ankyrin repeats (3 copies)

  2. XM_006515048.2 → XP_006515111.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X2

    UniProtKB/TrEMBL
    Q3UH27
    Conserved Domains (6) summary
    cd07642
    Location:34 → 248
    BAR_ASAP2; The Bin/Amphiphysin/Rvs (BAR) domain of ArfGAP with SH3 domain, ANK repeat and PH domain containing protein 2
    cd11966
    Location:942 → 997
    SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
    cd13251
    Location:297 → 403
    PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
    sd00045
    Location:586 → 618
    ANK; ANK repeat [structural motif]
    cd08849
    Location:419 → 541
    ArfGap_ASAP2; ArfGAP domain of ASAP2 (ArfGAP2 with SH3 domain, ANK repeat and PH domain-containing protein 2)
    pfam12796
    Location:589 → 673
    Ank_2; Ankyrin repeats (3 copies)

  3. XM_036157300.1 → XP_036013193.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X4

    Conserved Domains (2) summary
    cl12013
    Location:34 → 248
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
    cl17171
    Location:300 → 354
    PH-like; Pleckstrin homology-like domain

  4. XM_036157302.1 → XP_036013195.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X6

    Conserved Domains (1) summary
    cl12013
    Location:34 → 248
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature

  5. XM_036157301.1 → XP_036013194.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X5

    Conserved Domains (2) summary
    cl12013
    Location:34 → 248
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature
    cl17171
    Location:297 → 351
    PH-like; Pleckstrin homology-like domain

  6. XM_030246641.2 → XP_030102501.1  arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X3

    UniProtKB/TrEMBL
    Q3UH27
    Conserved Domains (6) summary
    cd11966
    Location:827 → 882
    SH3_ASAP2; Src homology 3 domain of ArfGAP with SH3 domain, ankyrin repeat and PH domain containing protein 2
    cd13251
    Location:182 → 288
    PH_ASAP; ArfGAP with SH3 domain, ankyrin repeat and PH domain Pleckstrin homology (PH) domain
    sd00045
    Location:471 → 503
    ANK; ANK repeat [structural motif]
    cd08849
    Location:304 → 426
    ArfGap_ASAP2; ArfGAP domain of ASAP2 (ArfGAP2 with SH3 domain, ANK repeat and PH domain-containing protein 2)
    pfam12796
    Location:474 → 558
    Ank_2; Ankyrin repeats (3 copies)
    cl12013
    Location:1 → 130
    BAR; The Bin/Amphiphysin/Rvs (BAR) domain, a dimerization module that binds membranes and detects membrane curvature

RNA

  1. XR_001780443.2 RNA Sequence

  2. XR_004937480.1 RNA Sequence

  3. XR_004937481.1 RNA Sequence

Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q7SIG6.3 GenPept UniProtKB/Swiss-Prot:Q7SIG6

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