ALOX5 arachidonate 5-lipoxygenase [Homo sapiens (human)] - Gene

Summary

Official Symbol: ALOX5provided by HGNC
Official Full Name: arachidonate 5-lipoxygenaseprovided by HGNC
Primary source: HGNC:HGNC:435
See related: Ensembl:ENSG00000012779 MIM:152390; AllianceGenome:HGNC:435
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: 5-LO; 5LPG; LOG5; 5-LOX
Summary: This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Expression: Broad expression in bone marrow (RPKM 35.1), lung (RPKM 26.8) and 15 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 10q11.21

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2023_10	current	GRCh38.p14 (GCF_000001405.40)	10	NC_000010.11 (45374216..45446117)
RS_2023_10	current	T2T-CHM13v2.0 (GCF_009914755.1)	10	NC_060934.1 (46255243..46327106)
105.20220307	previous assembly	GRCh37.p13 (GCF_000001405.25)	10	NC_000010.10 (45869664..45941565)

Chromosome 10 - NC_000010.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

ALOX5 drives the pyroptosis of CD4[+] T cells and tissue inflammation in rheumatoid arthritis.
Title: ALOX5 drives the pyroptosis of CD4(+) T cells and tissue inflammation in rheumatoid arthritis.
Cancer-educated neutrophils promote lung cancer progression via PARP-1-ALOX5-mediated MMP-9 expression.
Title: Cancer-educated neutrophils promote lung cancer progression via PARP-1-ALOX5-mediated MMP-9 expression.
GPR146 regulates pulmonary vascular remodeling by promoting pulmonary artery smooth muscle cell proliferation through 5-lipoxygenase.
Title: GPR146 regulates pulmonary vascular remodeling by promoting pulmonary artery smooth muscle cell proliferation through 5-lipoxygenase.
Oncogenic PIK3CA recruits myeloid-derived suppressor cells to shape the immunosuppressive tumour microenvironment in luminal breast cancer through the 5-lipoxygenase-dependent arachidonic acid pathway.
Title: Oncogenic PIK3CA recruits myeloid-derived suppressor cells to shape the immunosuppressive tumour microenvironment in luminal breast cancer through the 5-lipoxygenase-dependent arachidonic acid pathway.
Association between a single nucleotide polymorphism of the ALOX5 gene and susceptibility to multisystem tuberculosis in a Chinese Han population.
Title: Association between a single nucleotide polymorphism of the ALOX5 gene and susceptibility to multisystem tuberculosis in a Chinese Han population.
ALOX5 promotes autophagy-dependent ferroptosis by activating the AMPK/mTOR pathway in melanoma.
Title: ALOX5 promotes autophagy-dependent ferroptosis by activating the AMPK/mTOR pathway in melanoma.
Role of ALOX5 in non-small cell lung cancer: A potential therapeutic target associated with immune cell infiltration.
Title: Role of ALOX5 in non-small cell lung cancer: A potential therapeutic target associated with immune cell infiltration.
Exosomes derived from human adipose-derived stem cells alleviate hepatic ischemia-reperfusion (I/R) injury through the miR-183/ALOX5 axis.
Title: Exosomes derived from human adipose-derived stem cells alleviate hepatic ischemia-reperfusion (I/R) injury through the miR-183/ALOX5 axis.
Knock-out of 5-lipoxygenase in overexpressing tumor cells-consequences on gene expression and cellular function.
Title: Knock-out of 5-lipoxygenase in overexpressing tumor cells-consequences on gene expression and cellular function.
Iron Dyshomeostasis in COVID-19: Biomarkers Reveal a Functional Link to 5-Lipoxygenase Activation.
Title: Iron Dyshomeostasis in COVID-19: Biomarkers Reveal a Functional Link to 5-Lipoxygenase Activation.

Submit: New GeneRIF Correction See all GeneRIFs (243)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Associated conditions

Description	Tests
Inherited susceptibility to asthma MedGen: C1869116 OMIM: 600807 GeneReviews: Not available	Compare labs

EBI GWAS Catalog

Description
Discovery and refinement of loci associated with lipid levels. EBI GWAS Catalog EBI GWAS CatalogPubMed
Genome-wide association study of hematological and biochemical traits in a Japanese population. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
Envelope surface glycoprotein gp120	env	HIV-1 gp120 downregulates the expression of arachidonate 5-lipoxygenase (ALOX5) in human B cells	PubMed
	env	HIV-1 gp120 increases the intracellular concentrations of prostaglandin E2 and leukotriene B4 by upregulating the activity and expression of the arachidonate-metabolizing enzymes prostaglandin H synthase and 5-lipoxygenase, respectively	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

ALOX5 (e-PCR)
- UniSTS:503056

RH79041 (e-PCR)
- UniSTS:44581

D11S2467 (e-PCR)
- UniSTS:151847

RH102744 (e-PCR)
- UniSTS:97078

UniSTS:60247 (e-PCR)
- UniSTS:60247

RH17822 (e-PCR)
- UniSTS:71005

SHGC-59903 (e-PCR)
- UniSTS:34693

RH98891 (e-PCR)
- UniSTS:91527

D10S1413 (e-PCR)
- UniSTS:52128

L18441 (e-PCR)
- UniSTS:71348

SHGC-60141 (e-PCR)
- UniSTS:32451

D11S3553 (e-PCR)
- UniSTS:152792

WI-9162 (e-PCR)
- UniSTS:78319

RH40180 (e-PCR)
- UniSTS:87536

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables arachidonate 12(S)-lipoxygenase activity	IEA Inferred from Electronic Annotation more info
enables arachidonate 5-lipoxygenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables arachidonate 5-lipoxygenase activity	IDA Inferred from Direct Assay more info	PubMed
enables arachidonate 5-lipoxygenase activity	TAS Traceable Author Statement more info
enables arachidonate 8(S)-lipoxygenase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables iron ion binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in arachidonic acid metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in dendritic cell migration	ISS Inferred from Sequence or Structural Similarity more info
involved_in glucose homeostasis	ISS Inferred from Sequence or Structural Similarity more info
involved_in humoral immune response	ISS Inferred from Sequence or Structural Similarity more info
involved_in leukocyte chemotaxis involved in inflammatory response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in leukocyte migration involved in inflammatory response	ISS Inferred from Sequence or Structural Similarity more info
involved_in leukotriene A4 biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in leukotriene A4 biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in leukotriene biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in leukotriene metabolic process	TAS Traceable Author Statement more info	PubMed
involved_in leukotriene production involved in inflammatory response	IEA Inferred from Electronic Annotation more info
involved_in lipid oxidation	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipoxin biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lipoxygenase pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipoxygenase pathway	TAS Traceable Author Statement more info
involved_in long-chain fatty acid biosynthetic process	TAS Traceable Author Statement more info
involved_in negative regulation of angiogenesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of endothelial cell proliferation	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of inflammatory response	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of response to endoplasmic reticulum stress	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of sprouting angiogenesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of vascular wound healing	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of wound healing	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of bone mineralization	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of leukocyte adhesion to arterial endothelial cell	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of cellular response to oxidative stress	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of cytokine production involved in inflammatory response	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of fat cell differentiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of inflammatory response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of inflammatory response to wounding	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of insulin secretion	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of reactive oxygen species biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	TAS Traceable Author Statement more info
located_in extracellular region	TAS Traceable Author Statement more info
located_in extracellular space	HDA more info	PubMed
located_in ficolin-1-rich granule lumen	TAS Traceable Author Statement more info
is_active_in nuclear envelope	IBA Inferred from Biological aspect of Ancestor more info
located_in nuclear envelope	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear envelope	TAS Traceable Author Statement more info
located_in nuclear envelope lumen	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear matrix	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear membrane	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	IDA Inferred from Direct Assay more info
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in secretory granule lumen	TAS Traceable Author Statement more info

General protein information

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Preferred Names: polyunsaturated fatty acid 5-lipoxygenase

Names: LOX-5; arachidonic acid 5-lipoxygenase; leukotriene A4 synthase

NP_000689.1

EC 1.13.11.34

NP_001243082.1

EC 1.13.11.34

NP_001243083.1

EC 1.13.11.34

NP_001307790.1

EC 1.13.11.34

NP_001307791.1

EC 1.13.11.34

XP_047280890.1

EC 1.13.11.34

XP_047280892.1

EC 1.13.11.34

XP_047280893.1

EC 1.13.11.34

XP_054185533.1

EC 1.13.11.34

XP_054185534.1

EC 1.13.11.34

XP_054185535.1

EC 1.13.11.34

XP_054221354.1

EC 1.13.11.34

XP_054221355.1

EC 1.13.11.34

XP_054221356.1

EC 1.13.11.34

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_011437.1 RefSeqGene

Range

5036..76937

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_000698.5 → NP_000689.1 polyunsaturated fatty acid 5-lipoxygenase isoform 1

See identical proteins and their annotated locations for NP_000689.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes the longest isoform (1).

Source sequence(s)

AW403395, BC132677

Consensus CDS

CCDS7212.1

UniProtKB/Swiss-Prot

B7ZLS0, E5FPY5, E5FPY7, E5FPY8, P09917, Q5JQ14

Related

ENSP00000363512.2, ENST00000374391.7

Conserved Domains (2) summary

cd01753
Location:2 → 115

PLAT_LOX; PLAT domain of 12/15-lipoxygenase. As a unique subfamily of the mammalian lipoxygenases, they catalyze enzymatic lipid peroxidation in complex biological structures via direct dioxygenation of phospholipids and cholesterol esters of biomembranes and ...

cl27717
Location:227 → 665

Lipoxygenase; Lipoxygenase
NM_001256153.3 → NP_001243082.1 polyunsaturated fatty acid 5-lipoxygenase isoform 2

See identical proteins and their annotated locations for NP_001243082.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks an in-frame segment in the coding region, compared to variant 1. The resulting isoform (2) is shorter than isoform 1.

Source sequence(s)

AK225603, AK315223, AW403395, BC132677

UniProtKB/Swiss-Prot

P09917

Conserved Domains (2) summary

cd01753
Location:2 → 115

PLAT_LOX; PLAT domain of 12/15-lipoxygenase. As a unique subfamily of the mammalian lipoxygenases, they catalyze enzymatic lipid peroxidation in complex biological structures via direct dioxygenation of phospholipids and cholesterol esters of biomembranes and ...

pfam00305
Location:227 → 633

Lipoxygenase; Lipoxygenase
NM_001256154.3 → NP_001243083.1 polyunsaturated fatty acid 5-lipoxygenase isoform 3

See identical proteins and their annotated locations for NP_001243083.1

Status: REVIEWED

Description

Transcript Variant: This variant (3) lacks an in-frame exon in the 3' coding region, compared to variant 1. The resulting isoform (3) is shorter than isoform 1.

Source sequence(s)

AW403395, BC143985

Consensus CDS

CCDS58078.1

UniProtKB/Swiss-Prot

P09917

Related

ENSP00000437634.1, ENST00000542434.5

Conserved Domains (2) summary

cd01753
Location:2 → 115

PLAT_LOX; PLAT domain of 12/15-lipoxygenase. As a unique subfamily of the mammalian lipoxygenases, they catalyze enzymatic lipid peroxidation in complex biological structures via direct dioxygenation of phospholipids and cholesterol esters of biomembranes and ...

pfam00305
Location:227 → 608

Lipoxygenase; Lipoxygenase
NM_001320861.2 → NP_001307790.1 polyunsaturated fatty acid 5-lipoxygenase isoform 4

Status: REVIEWED

Description

Transcript Variant: This variant (4) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. The encoded isoform (4) is shorter than isoform 1.

Source sequence(s)

AL731567, AW403395, BC132677

UniProtKB/Swiss-Prot

P09917

Conserved Domains (2) summary

cd01753
Location:2 → 115

PLAT_LOX; PLAT domain of 12/15-lipoxygenase. As a unique subfamily of the mammalian lipoxygenases, they catalyze enzymatic lipid peroxidation in complex biological structures via direct dioxygenation of phospholipids and cholesterol esters of biomembranes and ...

pfam00305
Location:227 → 636

Lipoxygenase; Lipoxygenase
NM_001320862.2 → NP_001307791.1 polyunsaturated fatty acid 5-lipoxygenase isoform 5

Status: REVIEWED

Description

Transcript Variant: This variant (5) differs in the 5' UTR and initiates translation at a downstream start site compared to variant 1. The encoded isoform (5) has a shorter N-terminus than isoform 1.

Source sequence(s)

AW403395, BC143986

Conserved Domains (1) summary

pfam00305
Location:82 → 520

Lipoxygenase; Lipoxygenase

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

NC_000010.11 Reference GRCh38.p14 Primary Assembly

Range

45374216..45446117

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_047424934.1 → XP_047280890.1 polyunsaturated fatty acid 5-lipoxygenase isoform X1
XM_047424936.1 → XP_047280892.1 polyunsaturated fatty acid 5-lipoxygenase isoform X2
XM_047424937.1 → XP_047280893.1 polyunsaturated fatty acid 5-lipoxygenase isoform X3

Reference GRCh38.p14 ALT_REF_LOCI_1

Genomic

NW_003315935.1 Reference GRCh38.p14 ALT_REF_LOCI_1

Range

215047..286948

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_054329558.1 → XP_054185533.1 polyunsaturated fatty acid 5-lipoxygenase isoform X1
XM_054329559.1 → XP_054185534.1 polyunsaturated fatty acid 5-lipoxygenase isoform X2
XM_054329560.1 → XP_054185535.1 polyunsaturated fatty acid 5-lipoxygenase isoform X3

Alternate T2T-CHM13v2.0

Genomic

NC_060934.1 Alternate T2T-CHM13v2.0

Range

46255243..46327106

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_054365379.1 → XP_054221354.1 polyunsaturated fatty acid 5-lipoxygenase isoform X1
XM_054365380.1 → XP_054221355.1 polyunsaturated fatty acid 5-lipoxygenase isoform X2
XM_054365381.1 → XP_054221356.1 polyunsaturated fatty acid 5-lipoxygenase isoform X3