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NT5E 5'-nucleotidase ecto [ Homo sapiens (human) ]

Gene ID: 4907, updated on 5-Mar-2024

Summary

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Official Symbol
NT5Eprovided by HGNC
Official Full Name
5'-nucleotidase ectoprovided by HGNC
Primary source
HGNC:HGNC:8021
See related
Ensembl:ENSG00000135318 MIM:129190; AllianceGenome:HGNC:8021
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA
Summary
The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
Expression
Ubiquitous expression in endometrium (RPKM 30.0), ovary (RPKM 19.6) and 22 other tissues See more
Orthologs
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Genomic context

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Location:
6q14.3
Exon count:
9
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 6 NC_000006.12 (85450083..85495784)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 6 NC_060930.1 (86666882..86712587)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 6 NC_000006.11 (86159801..86205502)

Chromosome 6 - NC_000006.12Genomic Context describing neighboring genes Neighboring gene TPT1 pseudogene 6 Neighboring gene deoxyuridine triphosphatase pseudogene 5 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 17366 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 24787 Neighboring gene ReSE screen-validated silencer GRCh37_chr6:86210956-86211197 Neighboring gene sorting nexin 14 Neighboring gene Sharpr-MPRA regulatory region 13581 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr6:86262889-86263390 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr6:86263391-86263890 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr6:86302067-86302652 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr6:86302653-86303236 Neighboring gene H3K27ac hESC enhancer GRCh37_chr6:86303237-86303820 Neighboring gene synaptotagmin binding cytoplasmic RNA interacting protein Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 17368 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 17369 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 17370

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. The CD73 immune checkpoint promotes tumor cell metabolic fitness. Allard D, et al. Elife, 2023 Jun 1. PMID 37261423, Free PMC Article
  2. NT5E DNA methylation in papillary thyroid cancer: Novel opportunities for precision oncology. Bertoni APS, et al. Mol Cell Endocrinol, 2023 Jun 15. PMID 37059175
  3. CD73 in glioblastoma: Where are we now and what are the future directions? Gelsleichter NE, et al. Immunol Lett, 2023 Apr-May. PMID 36958430
  4. Single-Cell Phenotyping of CD73 Expression Reveals the Diversity of the Tumor Immune Microenvironment and Reflects the Prognosis of Bladder Cancer. Izawa M, et al. Lab Invest, 2023 Apr. PMID 36870289
  5. CD73/NT5E-mediated ubiquitination of AURKA regulates alcohol-related liver fibrosis via modulating hepatic stellate cell senescence. Liu Z, et al. Int J Biol Sci, 2023. PMID 36778123, Free PMC Article

See all (290) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. CD73 as a T cell dysfunction marker predicting cardiovascular and infection events in patients undergoing hemodialysis.
    Title: CD73 as a T cell dysfunction marker predicting cardiovascular and infection events in patients undergoing hemodialysis.
  2. Altered CD39 and CD73 Expression in Rheumatoid Arthritis: Implications for Disease Activity and Treatment Response.
    Title: Altered CD39 and CD73 Expression in Rheumatoid Arthritis: Implications for Disease Activity and Treatment Response.
  3. Human plasmacytoid dendritic cells express the functional purinergic halo (CD39/CD73).
    Title: Human plasmacytoid dendritic cells express the functional purinergic halo (CD39/CD73).
  4. CD73 contributes to the pathogenesis of fusion-negative rhabdomyosarcoma through the purinergic signaling pathway.
    Title: CD73 contributes to the pathogenesis of fusion-negative rhabdomyosarcoma through the purinergic signaling pathway.
  5. The hypoxia-regulated ectonucleotidase CD73 is a host determinant of HIV latency.
    Title: The hypoxia-regulated ectonucleotidase CD73 is a host determinant of HIV latency.
  6. Effects of abnormal expression of CD73 on malignant phenotype of nasopharyngeal carcinoma.
    Title: Effects of abnormal expression of CD73 on malignant phenotype of nasopharyngeal carcinoma.
  7. CD73 controls Myosin II-driven invasion, metastasis, and immunosuppression in amoeboid pancreatic cancer cells.
    Title: CD73 controls Myosin II-driven invasion, metastasis, and immunosuppression in amoeboid pancreatic cancer cells.
  8. Integrative analyses identify CD73 as a prognostic biomarker and immunotherapeutic target in intrahepatic cholangiocarcinoma.
    Title: Integrative analyses identify CD73 as a prognostic biomarker and immunotherapeutic target in intrahepatic cholangiocarcinoma.
  9. 5'-Ectonucleotidase CD73/NT5E supports EGFR-mediated invasion of HPV-negative head and neck carcinoma cells.
    Title: 5'-Ectonucleotidase CD73/NT5E supports EGFR-mediated invasion of HPV-negative head and neck carcinoma cells.
  10. The CD73 is induced by TGF-beta1 triggered by nutrient deprivation and highly expressed in dedifferentiated human melanoma.
    Title: The CD73 is induced by TGF-β1 triggered by nutrient deprivation and highly expressed in dedifferentiated human melanoma.
Submit: New GeneRIF Correction See all GeneRIFs (229)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Associated conditions

Description Tests
Hereditary arterial and articular multiple calcification syndrome
MedGen: C1859372 OMIM: 211800 GeneReviews: Not available
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EBI GWAS Catalog

Description
An atlas of genetic influences on human blood metabolites.
EBI GWAS Catalog
Human metabolic individuality in biomedical and pharmaceutical research.
EBI GWAS Catalog

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

HIV-1 interactions

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Protein interactions

Protein Gene Interaction Pubs
Vif vif HIV-1 Vif upregulates the expression of 5'-nucleotidase, ecto (NT5E, CD73) in Vif-expression T cells PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables 5'-deoxynucleotidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 5'-nucleotidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 5'-nucleotidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 5'-nucleotidase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables GMP 5'-nucleotidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables IMP 5'-nucleotidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables XMP 5'-nucleosidase activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables thymidylate 5'-phosphatase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables zinc ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in ADP catabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in AMP catabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in ATP metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in DNA metabolic process TAS
Traceable Author Statement
more info
 PubMed 
involved_in adenosine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in calcium ion homeostasis IEA
Inferred from Electronic Annotation
more info
  
involved_in inhibition of non-skeletal tissue mineralization IEA
Inferred from Electronic Annotation
more info
  
involved_in leukocyte cell-cell adhesion IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in negative regulation of inflammatory response IEA
Inferred from Electronic Annotation
more info
  
involved_in response to ATP IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in response to inorganic substance IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in cell surface HDA PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
  
located_in external side of plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in extracellular exosome HDA PubMed 
located_in membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleoplasm IDA
Inferred from Direct Assay
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IDA
Inferred from Direct Assay
more info
  
located_in plasma membrane TAS
Traceable Author Statement
more info
  

General protein information

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Preferred Names
5'-nucleotidase
Names
5'-NT
5'-deoxynucleotidase
IMP-specific 5'-nucleotidase
Purine 5-Prime-Nucleotidase
ecto-5'-nucleotidase
thymidylate 5'-phosphatase
NP_001191742.1
NP_002517.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_028214.1 RefSeqGene

    Range
    5500..51201
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001204813.2 → NP_001191742.1  5'-nucleotidase isoform 2 preproprotein

    See identical proteins and their annotated locations for NP_001191742.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.
    Source sequence(s)
    AK075008, BC065937, BQ019358, DA574510, X55740
    Consensus CDS
    CCDS56439.1
    UniProtKB/TrEMBL
    B2RBH2
    Related
    ENSP00000358665.3, ENST00000369651.7
    Conserved Domains (3) summary
    COG0737
    Location:11 → 482
    UshA; 2',3'-cyclic-nucleotide 2'-phosphodiesterase/5'- or 3'-nucleotidase, 5'-nucleotidase family [Nucleotide transport and metabolism, Defense mechanisms]
    cd07409
    Location:29 → 310
    MPP_CD73_N; CD73 ecto-5'-nucleotidase and related proteins, N-terminal metallophosphatase domain
    pfam02872
    Location:339 → 457
    5_nucleotid_C; 5'-nucleotidase, C-terminal domain

  2. NM_002526.4 → NP_002517.1  5'-nucleotidase isoform 1 preproprotein

    See identical proteins and their annotated locations for NP_002517.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
    Source sequence(s)
    AK075008, BC065937, BQ019358, DA574510, X55740
    Consensus CDS
    CCDS5002.1
    UniProtKB/Swiss-Prot
    B3KQI8, O75520, P21589, Q5W116
    UniProtKB/TrEMBL
    B2RBH2
    Related
    ENSP00000257770.3, ENST00000257770.8
    Conserved Domains (3) summary
    COG0737
    Location:11 → 532
    UshA; 2',3'-cyclic-nucleotide 2'-phosphodiesterase/5'- or 3'-nucleotidase, 5'-nucleotidase family [Nucleotide transport and metabolism, Defense mechanisms]
    cd07409
    Location:29 → 310
    MPP_CD73_N; CD73 ecto-5'-nucleotidase and related proteins, N-terminal metallophosphatase domain
    pfam02872
    Location:339 → 507
    5_nucleotid_C; 5'-nucleotidase, C-terminal domain

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000006.12 Reference GRCh38.p14 Primary Assembly

    Range
    85450083..85495784
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060930.1 Alternate T2T-CHM13v2.0

    Range
    86666882..86712587
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P21589.1 GenPept UniProtKB/Swiss-Prot:P21589

Gene LinkOut

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