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APIP APAF1 interacting protein [ Homo sapiens (human) ]

Gene ID: 51074, updated on 16-Apr-2024

Summary

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Official Symbol
APIPprovided by HGNC
Official Full Name
APAF1 interacting proteinprovided by HGNC
Primary source
HGNC:HGNC:17581
See related
Ensembl:ENSG00000149089 MIM:612491; AllianceGenome:HGNC:17581
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
APIP2; CGI29; hAPIP; CGI-29; MMRP19
Summary
Enables identical protein binding activity; methylthioribulose 1-phosphate dehydratase activity; and zinc ion binding activity. Involved in several processes, including L-methionine salvage from methylthioadenosine; protein homotetramerization; and pyroptosis. Located in cytoplasm. [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in fat (RPKM 15.7), adrenal (RPKM 13.7) and 25 other tissues See more
Orthologs
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Genomic context

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See APIP in Genome Data Viewer
Location:
11p13
Exon count:
9
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 11 NC_000011.10 (34882295..34916379, complement)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 11 NC_060935.1 (35020271..35054314, complement)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 11 NC_000011.9 (34903842..34937926, complement)

Chromosome 11 - NC_000011.10Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC102723568 Neighboring gene uncharacterized LOC105376625 Neighboring gene uncharacterized LOC105376624 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 3253 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 3254 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 4608 Neighboring gene pyruvate dehydrogenase complex component X Neighboring gene microRNA 1343 Neighboring gene uncharacterized LOC105376626 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 4609 Neighboring gene MED14-independent group 3 enhancer GRCh37_chr11:35051560-35052759 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 4611 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 4612

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. Crystallization and preliminary X-ray crystallographic analysis of human Apaf-1-interacting protein. Kang W, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2012 Dec 1. PMID 23192037, Free PMC Article
  2. Structural and biochemical basis for the inhibition of cell death by APIP, a methionine salvage enzyme. Kang W, et al. Proc Natl Acad Sci U S A, 2014 Jan 7. PMID 24367089, Free PMC Article
  3. Functional identification of APIP as human mtnB, a key enzyme in the methionine salvage pathway. Mary C, et al. PLoS One, 2012. PMID 23285211, Free PMC Article
  4. Down-regulated expression of apoptosis-associated genes APIP and UACA in non-small cell lung carcinoma. Moravcikova E, et al. Int J Oncol, 2012 Jun. PMID 22407486
  5. Cardioprotective role of APIP in myocardial infarction through ADORA2B. Lim B, et al. Cell Death Dis, 2019 Jul 1. PMID 31263105, Free PMC Article

See all (35) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. APIP regulated by YAP propels methionine cycle and metastasis in head and neck squamous cell carcinoma.
    Title: APIP regulated by YAP propels methionine cycle and metastasis in head and neck squamous cell carcinoma.
  2. APIP is crucial for cardioprotection against myocardial infarction by virtue of binding to and stabilizing ADORA2B, thereby dampening ischemic heart injury.
    Title: Cardioprotective role of APIP in myocardial infarction through ADORA2B.
  3. Results suggest that APIP stimulates cell proliferation via ERBB3, acting in conjunction with ERBB2, in gastric cancer cells.
    Title: APIP, an ERBB3-binding partner, stimulates erbB2-3 heterodimer formation to promote tumorigenesis.
  4. APIP/MtnB functions as a cell death inhibitor independently of its MtnB enzyme activity for apoptosis induced by either hypoxia or etoposide, but dependently for caspase-1-induced pyroptosis
    Title: Structural and biochemical basis for the inhibition of cell death by APIP, a methionine salvage enzyme.
  5. these results confirm the involvement of APIP in the methionine salvage pathway, which plays a key role in many biological functions like cancer, apoptosis, microbial proliferation and inflammation.
    Title: Functional identification of APIP as human mtnB, a key enzyme in the methionine salvage pathway.
  6. human APIP was overproduced in Escherichia coli, purified and crystallized. An X-ray diffraction data set was collected to 2.40 A resolution
    Title: Crystallization and preliminary X-ray crystallographic analysis of human Apaf-1-interacting protein.
  7. A polymorphism associated with reduced expression of a putative methionine salvage pathway dehydratase, apoptotic protease activating factor 1 (APAF1)-interacting protein (APIP), was associated with increased caspase-1-mediated cell death in Salmonella.
    Title: Functional genetic screen of human diversity reveals that a methionine salvage enzyme regulates inflammatory cell death.
  8. The down-regulation of APIP and UACA expression suggests that the threshold to activate the apoptosome apparatus may be decreased in non-small cell lung cancer cells.
    Title: Down-regulated expression of apoptosis-associated genes APIP and UACA in non-small cell lung carcinoma.
  9. Observational study of gene-disease association. (HuGE Navigator)
    Title: Investigation of genetic susceptibility factors for human longevity - a targeted nonsynonymous SNP study.
  10. APIP functions to inhibit muscle ischemic damage by binding to Apaf-1 in the Apaf-1/caspase-9 apoptosis pathway.
    Title: Induced inhibition of ischemic/hypoxic injury by APIP, a novel Apaf-1-interacting protein.
Submit: New GeneRIF Correction

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
Genome-wide association analysis of blood biomarkers in chronic obstructive pulmonary disease.
EBI GWAS Catalog
Genome-wide association and linkage identify modifier loci of lung disease severity in cystic fibrosis at 11p13 and 20q13.2.
EBI GWAS Catalog

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • dJ179L10.2

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables methylthioribulose 1-phosphate dehydratase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables methylthioribulose 1-phosphate dehydratase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables zinc ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in L-methionine salvage from S-adenosylmethionine IEA
Inferred from Electronic Annotation
more info
  
involved_in L-methionine salvage from methylthioadenosine IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in L-methionine salvage from methylthioadenosine IEA
Inferred from Electronic Annotation
more info
  
involved_in L-methionine salvage from methylthioadenosine IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in apoptotic process IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of apoptotic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein homotetramerization IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in pyroptosis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of ERK1 and ERK2 cascade ISS
Inferred from Sequence or Structural Similarity
more info
  
Component Evidence Code Pubs
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytosol TAS
Traceable Author Statement
more info
  

General protein information

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Preferred Names
methylthioribulose-1-phosphate dehydratase
Names
MTRu-1-P dehydratase
probable methylthioribulose-1-phosphate dehydratase
NP_057041.2
XP_011518456.1
XP_016873364.1
XP_054225020.1
XP_054225021.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_015957.4 → NP_057041.2  methylthioribulose-1-phosphate dehydratase

    See identical proteins and their annotated locations for NP_057041.2

    Status: VALIDATED

    Source sequence(s)
    AC107928, AF088034, BC009077, BP200131
    Consensus CDS
    CCDS7895.1
    UniProtKB/Swiss-Prot
    A8K9D3, Q6PJX6, Q8WVU2, Q96GX9, Q96HK2, Q9Y318
    UniProtKB/TrEMBL
    B4DY17
    Related
    ENSP00000379133.3, ENST00000395787.4
    Conserved Domains (1) summary
    TIGR03328
    Location:28 → 227
    salvage_mtnB; methylthioribulose-1-phosphate dehydratase

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000011.10 Reference GRCh38.p14 Primary Assembly

    Range
    34882295..34916379 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_011520154.4 → XP_011518456.1  methylthioribulose-1-phosphate dehydratase isoform X1

    UniProtKB/TrEMBL
    B4DY17
    Conserved Domains (1) summary
    TIGR03328
    Location:45 → 244
    salvage_mtnB; methylthioribulose-1-phosphate dehydratase

  2. XM_017017875.3 → XP_016873364.1  methylthioribulose-1-phosphate dehydratase isoform X2

    UniProtKB/TrEMBL
    S4R3D6
    Related
    ENSP00000474191.1, ENST00000532428.6
    Conserved Domains (1) summary
    cl00214
    Location:1 → 155
    Aldolase_II; Class II Aldolase and Adducin head (N-terminal) domain. Aldolases are ubiquitous enzymes catalyzing central steps of carbohydrate metabolism. Based on enzymatic mechanisms, this superfamily has been divided into two distinct classes (Class I and II). ...

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060935.1 Alternate T2T-CHM13v2.0

    Range
    35020271..35054314 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_054369045.1 → XP_054225020.1  methylthioribulose-1-phosphate dehydratase isoform X1

  2. XM_054369046.1 → XP_054225021.1  methylthioribulose-1-phosphate dehydratase isoform X2

Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q96GX9.1 GenPept UniProtKB/Swiss-Prot:Q96GX9

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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