Hgs hepatocyte growth factor-regulated tyrosine kinase substrate [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Hgsprovided by RGD
Official Full Name: hepatocyte growth factor-regulated tyrosine kinase substrateprovided by RGD
Primary source: RGD:69225
See related: Ensembl:ENSRNOG00000036696 AllianceGenome:RGD:69225
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Hrs
Summary: Predicted to enable several functions, including identical protein binding activity; ubiquitin-dependent protein binding activity; and ubiquitin-like protein ligase binding activity. Involved in endosome to lysosome transport. Located in cytosol; membrane; and secretory granule. Orthologous to human HGS (hepatocyte growth factor-regulated tyrosine kinase substrate). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Kidney (RPKM 140.2), Lung (RPKM 139.4) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 10q32.3

Exon count:: 23

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	10	NC_086028.1 (106237988..106256174)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	10	NC_051345.1 (105739617..105757838)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	10	NC_005109.4 (109638944..109657460)

Chromosome 10 - NC_086028.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The distinct role of Hrs in promoting recycling of internalized TrkB-FL receptors is independent of its ubiquitin-interacting motif.
Title: Essential role of Hrs in endocytic recycling of full-length TrkB receptor but not its isoform TrkB.T1.
Nedd4-2 induces binding of ENaC to Hrs, which mediates the sorting decision between ENaC degradation and recycling.
Title: Hrs controls sorting of the epithelial Na+ channel between endosomal degradation and recycling pathways.
Hrs and Tsg101 as crucial regulators of trafficking of Cx43 to lysosomes.
Title: Ubiquitylation of the gap junction protein connexin-43 signals its trafficking from early endosomes to lysosomes in a process mediated by Hrs and Tsg101.
HRS can reduce the abundance of total and active EGFR and may reflect cell type-specific HRS function
Title: HRS inhibits EGF receptor signaling in the RT4 rat schwannoma cell line.
SNAP-25 is a receptor for Hrs on early endosomal membranes; the binding of Hrs to SNAP-25 on endosomal membranes inhibits formation of a SNARE complex required for homotypic endosome fusion.
Title: Hrs regulates early endosome fusion by inhibiting formation of an endosomal SNARE complex.
Huntingtin-associated protein 1 interacts with this protein and functions in endosomal trafficking
Title: Huntingtin-associated protein 1 interacts with hepatocyte growth factor-regulated tyrosine kinase substrate and functions in endosomal trafficking.
GRIF1 may participate in microtubule-based transport of early endosomes by acting as an adaptor linking Hrs-containing endosomes to kinesin.
Title: GRIF1 binds Hrs and is a new regulator of endosomal trafficking.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phosphatidylinositol binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	IEA Inferred from Electronic Annotation more info
enables protein domain specific binding	ISO Inferred from Sequence Orthology more info
enables protein domain specific binding	NAS Non-traceable Author Statement more info	PubMed
enables ubiquitin binding	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin binding	IEA Inferred from Electronic Annotation more info
enables ubiquitin-like protein ligase binding	IEA Inferred from Electronic Annotation more info
enables ubiquitin-like protein ligase binding	ISO Inferred from Sequence Orthology more info
enables ubiquitin-modified protein reader activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in endocytic recycling	IBA Inferred from Biological aspect of Ancestor more info
involved_in endosome to lysosome transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in membrane invagination	IEA Inferred from Electronic Annotation more info
involved_in membrane invagination	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of angiogenesis	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of angiogenesis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of platelet-derived growth factor receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of platelet-derived growth factor receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of receptor signaling pathway via JAK-STAT	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of receptor signaling pathway via JAK-STAT	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of vascular endothelial growth factor receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of vascular endothelial growth factor receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of exosomal secretion	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of exosomal secretion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of gene expression	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in protein localization to membrane	IEA Inferred from Electronic Annotation more info
involved_in protein localization to membrane	ISO Inferred from Sequence Orthology more info
involved_in protein targeting to lysosome	IEA Inferred from Electronic Annotation more info
involved_in protein targeting to lysosome	ISO Inferred from Sequence Orthology more info
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in receptor internalization	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of ESCRT-0 complex	IEA Inferred from Electronic Annotation more info
part_of ESCRT-0 complex	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in early endosome	IBA Inferred from Biological aspect of Ancestor more info
located_in early endosome	ISO Inferred from Sequence Orthology more info
located_in early endosome membrane	IEA Inferred from Electronic Annotation more info
located_in endosome	ISO Inferred from Sequence Orthology more info
located_in lysosome	IEA Inferred from Electronic Annotation more info
located_in lysosome	ISO Inferred from Sequence Orthology more info
located_in multivesicular body membrane	IEA Inferred from Electronic Annotation more info
located_in secretory granule	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: hepatocyte growth factor-regulated tyrosine kinase substrate

Names: HGF-regulated tyrosine kinase substrate; SNAP-25-interacting protein Hrs-2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001399619.1 → NP_001386548.1 hepatocyte growth factor-regulated tyrosine kinase substrate isoform 2

Status: VALIDATED

Source sequence(s)

JAXUCZ010000010

UniProtKB/Swiss-Prot

P97847, Q5XIV8, Q76N76, Q9JJ50

UniProtKB/TrEMBL

A6HLD6

Related

ENSRNOP00000087685.1, ENSRNOT00000110912.1
NM_001399620.1 → NP_001386549.1 hepatocyte growth factor-regulated tyrosine kinase substrate isoform 3

Status: VALIDATED

Source sequence(s)

JAXUCZ010000010

Related

ENSRNOP00000051849.5, ENSRNOT00000054966.5
NM_019387.3 → NP_062260.2 hepatocyte growth factor-regulated tyrosine kinase substrate isoform 1

See identical proteins and their annotated locations for NP_062260.2

Status: VALIDATED

Source sequence(s)

JAXUCZ010000010

UniProtKB/TrEMBL

A0A8I6A993

Conserved Domains (5) summary

cd03569
Location:4 → 145

VHS_Hrs_Vps27p; VHS domain family, Hrs and Vps27p subfamily; composed of Hrs (Hepatocyte growth factor-regulated tyrosine kinase substrate) and its yeast homolog Vps27p (vacuolar protein sorting). The VHS domain, an essential part of Hrs/Vps27p, has a superhelical ...

cd15720
Location:159 → 219

FYVE_Hrs; FYVE domain found in hepatocyte growth factor (HGF)-regulated tyrosine kinase substrate (Hrs) and similar proteins

pfam12210
Location:406 → 500

Hrs_helical; Hepatocyte growth factor-regulated tyrosine kinase substrate

cd16269
Location:530 → 541

GBP_C; coiled coil [structural motif]

cl20817
Location:485 → 559

GBP_C; Guanylate-binding protein, C-terminal domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086028.1 Reference GRCr8

Range

106237988..106256174

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039086708.2 → XP_038942636.1 hepatocyte growth factor-regulated tyrosine kinase substrate isoform X2

UniProtKB/TrEMBL

A0A140TAH1

Conserved Domains (5) summary

PLN03209
Location:283 → 441

PLN03209; translocon at the inner envelope of chloroplast subunit 62; Provisional

cd15720
Location:221 → 281

FYVE_Hrs; FYVE domain found in hepatocyte growth factor (HGF)-regulated tyrosine kinase substrate (Hrs) and similar proteins

cd03569
Location:68 → 205

VHS_Hrs; VHS (Vps27/Hrs/STAM) domain of Hepatocyte growth factor-regulated tyrosine kinase substrate, Hrs

pfam15991
Location:560 → 809

G_path_suppress; G-protein pathway suppressor

cd21387
Location:467 → 562

GAT_Hrs; non-canonical GAT domain found in metazoan hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) and similar proteins
XM_039086710.2 → XP_038942638.2 hepatocyte growth factor-regulated tyrosine kinase substrate isoform X3
XM_039086709.2 → XP_038942637.2 hepatocyte growth factor-regulated tyrosine kinase substrate isoform X1
XM_039086712.2 → XP_038942640.1 hepatocyte growth factor-regulated tyrosine kinase substrate isoform X4

UniProtKB/TrEMBL

A0A8I6A993

Conserved Domains (5) summary

cd15720
Location:92 → 152

FYVE_Hrs; FYVE domain found in hepatocyte growth factor (HGF)-regulated tyrosine kinase substrate (Hrs) and similar proteins

pfam13868
Location:407 → 492

TPH; Trichohyalin-plectin-homology domain

pfam15991
Location:431 → 680

G_path_suppress; G-protein pathway suppressor

cd21387
Location:338 → 433

GAT_Hrs; non-canonical GAT domain found in metazoan hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs) and similar proteins

cl02544
Location:1 → 76

VHS_ENTH_ANTH; VHS, ENTH and ANTH domain superfamily