Alox15 arachidonate 15-lipoxygenase [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Alox15provided by RGD
Official Full Name: arachidonate 15-lipoxygenaseprovided by RGD
Primary source: RGD:70493
See related: Ensembl:ENSRNOG00000019183 AllianceGenome:RGD:70493
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: 12-LOX; 15-LOX; Alox12; Alox12l
Summary: Enables hepoxilin A3 synthase activity; hepoxilin-epoxide hydrolase activity; and oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen. Involved in fatty acid metabolic process and positive regulation of heterotypic cell-cell adhesion. Located in cytosol. Biomarker of brain ischemia; congestive heart failure; and glomerulonephritis. Orthologous to human ALOX15 (arachidonate 15-lipoxygenase). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Spleen (RPKM 2807.1), Lung (RPKM 941.1) and 1 other tissue See more
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Genomic context

Location:: 10q24

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	10	NC_086028.1 (55559060..55567535, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	10	NC_051345.1 (55060169..55068885, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	10	NC_005109.4 (56953692..56962145, complement)

Chromosome 10 - NC_086028.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Photothermal lipolysis accelerates ECM production via macrophage-derived ALOX15-mediated p38 MAPK activation in fibroblasts.
Title: Photothermal lipolysis accelerates ECM production via macrophage-derived ALOX15-mediated p38 MAPK activation in fibroblasts.
Alox15 processing of DHA contributes to production of resolvin D1 and LTP at hippocampo-prefrontal cortical synapses and associated spatial working memory performance.
Title: Distribution of Alox15 in the Rat Brain and Its Role in Prefrontal Cortical Resolvin D1 Formation and Spatial Working Memory.
Consistent with the decreases in the 15-LOX metabolites, expression levels of the genes that encode the 15-LOXs, Alox15 and Alox15b, were largely reduced in Olmsted syndrome model rats.
Title: Decreases in 15-lipoxygenase metabolites in Olmsted syndrome model rats.
Hypoxia increase the production of ROS through the 15-Lipoxygenase/15-Hydroxyeicosatetraenoiccid pathway.
Title: Key Role of ROS in the Process of 15-Lipoxygenase/15-Hydroxyeicosatetraenoiccid-Induced Pulmonary Vascular Remodeling in Hypoxia Pulmonary Hypertension.
the increased expression of 12/15-LOX, predominantly in neurons, and elevated production of 12(S)-HETE and 15(S)-HETE in ischemic brain.
Title: 12/15-Lipoxygenase metabolites of arachidonic acid activate PPARγ: a possible neuroprotective effect in ischemic brain.
Alox15 mRNA levels were 4 times higher in iron-deficient than in iron-adequate pancreas and that Reg1a, Reg3a, and Reg3b mRNA levels were 17-36 times higher in iron-overloaded pancreas.
Title: Microarray analysis of rat pancreas reveals altered expression of Alox15 and regenerating islet-derived genes in response to iron deficiency and overload.
suggest that chronic hypoxia promotes monocyte infiltration into the vasculature and adhesion molecules upregulation in pulmonary arterial endothelium cells via a positive interaction between 15-LO/15-HETE and nuclear factor-kappaB
Title: 15-Lipoxygenase promotes chronic hypoxia-induced pulmonary artery inflammation via positive interaction with nuclear factor-κB.
Reciprocal regulation of HIF-1alpha and 15-Lipoxygenase/15-HETE promotes anti-apoptosis process in pulmonary artery smooth muscle cells during hypoxia.
Title: Reciprocal regulation of HIF-1α and 15-LO/15-HETE promotes anti-apoptosis process in pulmonary artery smooth muscle cells during hypoxia.
Results support the pro-apoptotic role of 15-lipoxygenase-1 and suggest that 15-lipoxygenase-1 could be a potential new target gene for the therapy of malignant glioma.
Title: 15-Lipoxygenase-1 induces lipid peroxidation and apoptosis, and improves survival in rat malignant glioma.
15-Lox1-15(S)-HETE axis activates EGFR via redox-sensitive manner, which in turn mediates Src-Jak2-STAT3-dependent MCP-1 expression leading to vascular wall remodeling
Title: 15-Lipoxygenase-1-enhanced Src-Janus kinase 2-signal transducer and activator of transcription 3 stimulation and monocyte chemoattractant protein-1 expression require redox-sensitive activation of epidermal growth factor receptor in vascular wall remodeling.

Submit: New GeneRIF Correction See all GeneRIFs (25)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH141184 (e-PCR)
- UniSTS:223180

PMC126639P2 (e-PCR)
- UniSTS:270551

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables arachidonate 12(S)-lipoxygenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables arachidonate 12(S)-lipoxygenase activity	IDA Inferred from Direct Assay more info	PubMed
enables arachidonate 12(S)-lipoxygenase activity	IEA Inferred from Electronic Annotation more info
enables arachidonate 12(S)-lipoxygenase activity	ISO Inferred from Sequence Orthology more info
enables arachidonate 15-lipoxygenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables arachidonate 15-lipoxygenase activity	IDA Inferred from Direct Assay more info	PubMed
enables arachidonate 15-lipoxygenase activity	IEA Inferred from Electronic Annotation more info
enables arachidonate 15-lipoxygenase activity	ISO Inferred from Sequence Orthology more info
enables hepoxilin A3 synthase activity	IDA Inferred from Direct Assay more info	PubMed
enables hepoxilin-epoxide hydrolase activity	IDA Inferred from Direct Assay more info	PubMed
enables iron ion binding	IEA Inferred from Electronic Annotation more info
enables iron ion binding	ISO Inferred from Sequence Orthology more info
enables iron ion binding	ISS Inferred from Sequence or Structural Similarity more info
enables linoleate 13S-lipoxygenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables linoleate 13S-lipoxygenase activity	ISO Inferred from Sequence Orthology more info
enables linoleate 13S-lipoxygenase activity	ISS Inferred from Sequence or Structural Similarity more info
enables lipid binding	IEA Inferred from Electronic Annotation more info
enables phosphatidylinositol-4,5-bisphosphate binding	ISO Inferred from Sequence Orthology more info
enables phosphatidylinositol-4,5-bisphosphate binding	ISS Inferred from Sequence or Structural Similarity more info
enables potassium channel inhibitor activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in apoptotic cell clearance	IEA Inferred from Electronic Annotation more info
involved_in apoptotic cell clearance	ISO Inferred from Sequence Orthology more info
involved_in apoptotic cell clearance	ISS Inferred from Sequence or Structural Similarity more info
involved_in arachidonic acid metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in arachidonic acid metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in arachidonic acid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in arachidonic acid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in arachidonic acid metabolic process	NAS Non-traceable Author Statement more info	PubMed
involved_in bone mineralization	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within bone mineralization	ISO Inferred from Sequence Orthology more info
involved_in bone mineralization	ISS Inferred from Sequence or Structural Similarity more info
involved_in cellular response to calcium ion	ISO Inferred from Sequence Orthology more info
involved_in cellular response to calcium ion	ISS Inferred from Sequence or Structural Similarity more info
involved_in cellular response to interleukin-13	ISO Inferred from Sequence Orthology more info
involved_in cellular response to interleukin-13	ISS Inferred from Sequence or Structural Similarity more info
involved_in fatty acid oxidation	ISO Inferred from Sequence Orthology more info
involved_in fatty acid oxidation	ISS Inferred from Sequence or Structural Similarity more info
involved_in hepoxilin biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in icosanoid biosynthetic process	NAS Non-traceable Author Statement more info	PubMed
involved_in linoleic acid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in linoleic acid metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in lipid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in lipid oxidation	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipid oxidation	IEA Inferred from Electronic Annotation more info
involved_in lipoxin A4 biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in lipoxin A4 biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in lipoxin A4 biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in lipoxygenase pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in lipoxygenase pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in lipoxygenase pathway	IEA Inferred from Electronic Annotation more info
involved_in lipoxygenase pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of adaptive immune response	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of adaptive immune response	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of adaptive immune response	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of cell volume	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within ossification	ISO Inferred from Sequence Orthology more info
involved_in ossification	ISS Inferred from Sequence or Structural Similarity more info
involved_in phosphatidylethanolamine biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in phosphatidylethanolamine biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in phosphatidylethanolamine biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of ERK1 and ERK2 cascade	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of ERK1 and ERK2 cascade	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of actin filament polymerization	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of actin filament polymerization	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of actin filament polymerization	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of cell-substrate adhesion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell-substrate adhesion	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of heterotypic cell-cell adhesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of engulfment of apoptotic cell	IEA Inferred from Electronic Annotation more info
involved_in regulation of engulfment of apoptotic cell	ISO Inferred from Sequence Orthology more info
involved_in regulation of engulfment of apoptotic cell	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of inflammatory response	ISO Inferred from Sequence Orthology more info
involved_in regulation of inflammatory response	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of membrane potential	TAS Traceable Author Statement more info	PubMed
involved_in regulation of peroxisome proliferator activated receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in regulation of peroxisome proliferator activated receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in regulation of peroxisome proliferator activated receptor signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in response to endoplasmic reticulum stress	IEA Inferred from Electronic Annotation more info
involved_in response to endoplasmic reticulum stress	ISO Inferred from Sequence Orthology more info
involved_in response to endoplasmic reticulum stress	ISS Inferred from Sequence or Structural Similarity more info
involved_in wound healing	IEA Inferred from Electronic Annotation more info
involved_in wound healing	ISO Inferred from Sequence Orthology more info
involved_in wound healing	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic side of plasma membrane	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic side of plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in lipid droplet	IEA Inferred from Electronic Annotation more info
located_in lipid droplet	ISO Inferred from Sequence Orthology more info
located_in lipid droplet	ISS Inferred from Sequence or Structural Similarity more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in membrane	ISS Inferred from Sequence or Structural Similarity more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in sarcolemma	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: polyunsaturated fatty acid lipoxygenase ALOX15

Names: 12/15-LOX; 12/15-lipoxygenase; arachidonate 12-lipoxygenase, leukocyte-type; arachidonate omega-6 lipoxygenase; hepoxilin A3 synthase Alox15; linoleate 13S-lipoxygenase

NP_112272.2

EC 1.13.11.12

EC 1.13.11.31

EC 1.13.11.33

XP_006246886.1

EC 1.13.11.12

EC 1.13.11.31

EC 1.13.11.33

XP_038942858.1

EC 1.13.11.12

EC 1.13.11.31

EC 1.13.11.33

XP_038942860.1

EC 1.13.11.12

EC 1.13.11.31

EC 1.13.11.33

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_031010.2 → NP_112272.2 polyunsaturated fatty acid lipoxygenase ALOX15

See identical proteins and their annotated locations for NP_112272.2

Status: PROVISIONAL

Source sequence(s)

S69383

UniProtKB/Swiss-Prot

F1MA51, Q02759

Related

ENSRNOP00000026038.4, ENSRNOT00000026038.7

Conserved Domains (2) summary

cd01753
Location:2 → 112

PLAT_LOX; PLAT domain of 12/15-lipoxygenase. As a unique subfamily of the mammalian lipoxygenases, they catalyze enzymatic lipid peroxidation in complex biological structures via direct dioxygenation of phospholipids and cholesterol esters of biomembranes and ...

pfam00305
Location:214 → 654

Lipoxygenase; Lipoxygenase

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086028.1 Reference GRCr8

Range

55559060..55567535 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006246824.5 → XP_006246886.1 polyunsaturated fatty acid lipoxygenase ALOX15 isoform X2

UniProtKB/Swiss-Prot

F1MA51, Q02759

UniProtKB/TrEMBL

A0A8I6AV04

Related

ENSRNOP00000091973.1

Conserved Domains (2) summary

pfam00305
Location:244 → 684

Lipoxygenase; Lipoxygenase

cl00011
Location:75 → 142

PLAT; PLAT (Polycystin-1, Lipoxygenase, Alpha-Toxin) domain or LH2 (Lipoxygenase homology 2) domain. It consists of an eight stranded beta-barrel. The domain can be found in various domain architectures, in case of lipoxygenases, alpha toxin, lipases and ...
XM_039086930.2 → XP_038942858.1 polyunsaturated fatty acid lipoxygenase ALOX15 isoform X1

UniProtKB/Swiss-Prot

F1MA51, Q02759

Conserved Domains (2) summary

pfam00305
Location:295 → 747

Lipoxygenase

cl00011
Location:138 → 205

PLAT; PLAT (Polycystin-1, Lipoxygenase, Alpha-Toxin) domain or LH2 (Lipoxygenase homology 2) domain. It consists of an eight stranded beta-barrel. The domain can be found in various domain architectures, in case of lipoxygenases, alpha toxin, lipases and ...
XM_039086932.2 → XP_038942860.1 polyunsaturated fatty acid lipoxygenase ALOX15 isoform X3

Conserved Domains (1) summary

cl00011
Location:138 → 205

PLAT; PLAT (Polycystin-1, Lipoxygenase, Alpha-Toxin) domain or LH2 (Lipoxygenase homology 2) domain. It consists of an eight stranded beta-barrel. The domain can be found in various domain architectures, in case of lipoxygenases, alpha toxin, lipases and ...