YCF1 ATP-binding cassette glutathione S-conjugate transporter YCF1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: YCF1
Gene description: ATP-binding cassette glutathione S-conjugate transporter YCF1
Primary source: SGD:S000002542
Locus tag: YDR135C
See related: AllianceGenome:SGD:S000002542
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables ABC-type glutathione S-conjugate transporter activity and bilirubin transmembrane transporter activity. Involved in several processes, including bilirubin transport; cell redox homeostasis; and vacuole fusion, non-autophagic. Located in fungal-type vacuole membrane and membrane raft. Human ortholog(s) of this gene implicated in several diseases, including artery disease (multiple); bile duct disease (multiple); bilirubin metabolic disorder (multiple); leukemia (multiple); and lung disease (multiple). Orthologous to several human genes including ABCC1 (ATP binding cassette subfamily C member 1) and ABCC3 (ATP binding cassette subfamily C member 3). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (723004..727551, complement)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The structural basis for regulation of the glutathione transporter Ycf1 by regulatory domain phosphorylation.
Title: The structural basis for regulation of the glutathione transporter Ycf1 by regulatory domain phosphorylation.
Chaperonin point mutation enhances cadmium endurance in Saccharomyces cerevisiae.
Title: Chaperonin point mutation enhances cadmium endurance in Saccharomyces cerevisiae.
Structure of Ycf1p reveals the transmembrane domain TMD0 and the regulatory region of ABCC transporters.
Title: Structure of Ycf1p reveals the transmembrane domain TMD0 and the regulatory region of ABCC transporters.
Data indicate that deleting YCF1 caused a striking decrease in vacuolar levels of the soluble SNARE Vam7p, whereas total cellular levels were not altered.
Title: The yeast ATP-binding cassette (ABC) transporter Ycf1p enhances the recruitment of the soluble SNARE Vam7p to vacuoles for efficient membrane fusion.
Data show that Ycf1p plays an important role in cellular resistance to salt stress by maintaining the cellular redox balance via glutathione recycling.
Title: Ycf1p attenuates basal level oxidative stress response in Saccharomyces cerevisiae.
The authors provide strong evidence that Cka1p regulates Ycf1p function via phosphorylation of Ser251.
Title: Suppression of Ycf1p function by Cka1p-dependent phosphorylation is attenuated in response to salt stress.
Cka1p may regulate Ycf1p function through phosphorylation of Ser-251 either directly or indirectly.
Title: Negative regulation of the yeast ABC transporter Ycf1p by phosphorylation within its N-terminal extension.
Our data suggest that Ycf1p is involved in the maintenance of intracellular GSH homeostasis and it can interfere with the oxidative tolerance of yeast.
Title: The role of the yeast ATP-binding cassette Ycf1p in glutathione and cadmium ion homeostasis during respiratory metabolism.
Transport assays show that Tus1p, a guanine nucleotide exchange factor (GEF) for the small GTPase Rho1p, is a Rho1p-dependent-positive regulator of Ycf1p.
Title: Mapping protein-protein interactions for the yeast ABC transporter Ycf1p by integrated split-ubiquitin membrane yeast two-hybrid analysis.
An ATP-Binding Cassette transporter mediating the glutathione detoxification of heavy metals.
Title: Metal(loid)s and radionuclides cytotoxicity in Saccharomyces cerevisiae. Role of YCF1, glutathione and effect of buthionine sulfoximine.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ABC-type cadmium transporter activity	IEA Inferred from Electronic Annotation more info
enables ABC-type glutathione S-conjugate transporter activity	IDA Inferred from Direct Assay more info	PubMed
enables ABC-type glutathione S-conjugate transporter activity	IEA Inferred from Electronic Annotation more info
enables ABC-type transporter activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATPase-coupled transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables P-type cadmium transporter activity	IEA Inferred from Electronic Annotation more info
enables bilirubin transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in bilirubin transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cadmium ion transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in cell redox homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glutathione metabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in response to cadmium ion	IEA Inferred from Electronic Annotation more info
involved_in response to metal ion	IDA Inferred from Direct Assay more info	PubMed
involved_in transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in transmembrane transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vacuole fusion, non-autophagic	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in fungal-type vacuole membrane	HDA more info	PubMed
located_in fungal-type vacuole membrane	IDA Inferred from Direct Assay more info	PubMed
located_in fungal-type vacuole membrane	IEA Inferred from Electronic Annotation more info
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in membrane raft	IDA Inferred from Direct Assay more info	PubMed
located_in vacuolar membrane	IEA Inferred from Electronic Annotation more info
located_in vacuole	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: ATP-binding cassette glutathione S-conjugate transporter YCF1

NP_010419.3

EC 7.2.2.2

EC 7.6.2.3

Vacuolar glutathione S-conjugate transporter; ABC-C transporter of the ATP-binding cassette family; required for vacuole fusion; forms stable complexes with vacuole fusion machinery; regulates Vam7p recruitment to vacuoles; role in detoxifying metals (Cd, Hg, As); transports GSSG that is not immediately reduced in cytosol to vacuole; transports unconjugated bilirubin, selenodigluthatione, oxidized glutathione; similar to human cystic fibrosis protein CFTR

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

723004..727551 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180442.3 → NP_010419.3 TPA: ATP-binding cassette glutathione S-conjugate transporter YCF1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010419.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VSB9, P39109, Q03905

UniProtKB/TrEMBL

A0A8H8UN46, N1P6N0

Conserved Domains (1) summary

TIGR00957
Location:27 → 1511

MRP_assoc_pro; multi drug resistance-associated protein (MRP)