PRO1 glutamate 5-kinase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: PRO1
Gene description: glutamate 5-kinase
Primary source: SGD:S000002708
Locus tag: YDR300C
See related: AllianceGenome:SGD:S000002708
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables glutamate 5-kinase activity. Involved in proline biosynthetic process and ribophagy. Located in cytoplasm. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (1061505..1062791, complement)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

We show that PRO1 genetically interacts with UBP3, which encodes ubiquitin-specific protease, and is required for selective autophagy of ribosomes (ribophagy).
Title: γ-Glutamyl kinase is involved in selective autophagy of ribosomes in Saccharomyces cerevisiae.
Desensitization of feedback inhibition of the Saccharomyces cerevisiae gamma-glutamyl kinase enhances proline accumulation and freezing tolerance.
Title: Desensitization of feedback inhibition of the Saccharomyces cerevisiae gamma-glutamyl kinase enhances proline accumulation and freezing tolerance.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables glutamate 5-kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables glutamate 5-kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables glutamate 5-kinase activity	IEA Inferred from Electronic Annotation more info
enables glutamate 5-kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in L-proline biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in alpha-amino acid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in amino acid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in proline biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in proline biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in proline biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in ribophagy	IGI Inferred from Genetic Interaction more info	PubMed
involved_in ribophagy	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: glutamate 5-kinase

NP_010586.3

EC 2.7.2.11

Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; required for nitrogen starvation-induced ribophagy but not for nonselective autophagy; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

1061505..1062791 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180608.3 → NP_010586.3 TPA: glutamate 5-kinase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010586.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VSS9, P32264

UniProtKB/TrEMBL

A0A8H8UNK0, A6ZYP8, B3LFZ5, C7GQV0, C8Z5L2, N1PAH3

Conserved Domains (1) summary

COG0263
Location:7 → 426

ProB; Glutamate 5-kinase [Amino acid transport and metabolism]