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MIS1 trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase MIS1 [ Saccharomyces cerevisiae S288C ]

Gene ID: 852378, updated on 5-May-2024

Summary

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Gene symbol
MIS1
Gene description
trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase MIS1
Primary source
SGD:S000000288
Locus tag
YBR084W
See related
AllianceGenome:SGD:S000000288
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables formate-tetrahydrofolate ligase activity; methenyltetrahydrofolate cyclohydrolase activity; and methylenetetrahydrofolate dehydrogenase (NADP+) activity. Involved in folic acid biosynthetic process and nucleobase-containing compound metabolic process. Located in mitochondrion. Human ortholog(s) of this gene implicated in several diseases, including Down syndrome; cleft lip; developmental cardiac valvular defect; neural tube defect (multiple); and tetralogy of Fallot. Orthologous to several human genes including MTHFD1 (methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:
chromosome: II
Exon count:
1
Sequence:
Chromosome: II; NC_001134.8 (411054..413981)

Chromosome II - NC_001134.8Genomic Context describing neighboring genes Neighboring gene E2 ubiquitin-conjugating protein UBC4 Neighboring gene Tec1p Neighboring gene ribosomal 60S subunit protein L19A Neighboring gene ADP/ATP carrier protein AAC3

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Isolation and characterization of the Saccharomyces cerevisiae MIS1 gene encoding mitochondrial C1-tetrahydrofolate synthase. Shannon KW, et al. J Biol Chem, 1988 Jun 5. PMID 2836393
  2. Initiation of protein synthesis in Saccharomyces cerevisiae mitochondria without formylation of the initiator tRNA. Li Y, et al. J Bacteriol, 2000 May. PMID 10781559, Free PMC Article
  3. Purification and characterization of a mitochondrial isozyme of C1-tetrahydrofolate synthase from Saccharomyces cerevisiae. Shannon KW, et al. J Biol Chem, 1986 Sep 15. PMID 3528153
  4. Yeast AEP3p is an accessory factor in initiation of mitochondrial translation. Lee C, et al. J Biol Chem, 2009 Dec 4. PMID 19843529, Free PMC Article
  5. Autophagy facilitates adaptation of budding yeast to respiratory growth by recycling serine for one-carbon metabolism. May AI, et al. Nat Commun, 2020 Oct 7. PMID 33028817, Free PMC Article

See all (65) citations in PubMed

GeneRIFs: Gene References Into Functions

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables formate-tetrahydrofolate ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables formate-tetrahydrofolate ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables methenyltetrahydrofolate cyclohydrolase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables methenyltetrahydrofolate cyclohydrolase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables methenyltetrahydrofolate cyclohydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables methylenetetrahydrofolate dehydrogenase (NADP+) activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables methylenetetrahydrofolate dehydrogenase (NADP+) activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables methylenetetrahydrofolate dehydrogenase (NADP+) activity IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in folic acid biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in nucleobase-containing compound metabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in one-carbon metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in tetrahydrofolate interconversion IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in tetrahydrofolate interconversion IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in mitochondrion HDA PubMed 
is_active_in mitochondrion IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in mitochondrion IDA
Inferred from Direct Assay
more info
 PubMed 
located_in mitochondrion IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase MIS1
NP_009640.1
  • Mitochondrial C1-tetrahydrofolate synthase; involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001134.8 Reference assembly

    Range
    411054..413981
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001178432.1 → NP_009640.1  TPA: trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase MIS1 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_009640.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VQ83, P09440
    UniProtKB/TrEMBL
    A0A8H4BTF3, A6ZL30, G2W990, N1P8D0
    Conserved Domains (2) summary
    COG0190
    Location:37 → 327
    FolD; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase [Coenzyme transport and metabolism]
    pfam01268
    Location:345 → 975
    FTHFS; Formate--tetrahydrofolate ligase
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P09440.1

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