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TKL2 transketolase TKL2 [ Saccharomyces cerevisiae S288C ]

Gene ID: 852414, updated on 17-May-2024

Summary

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Gene symbol
TKL2
Gene description
transketolase TKL2
Primary source
FungiDB:YBR117C
Locus tag
YBR117C
See related
SGD:S000000321; AllianceGenome:SGD:S000000321
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Predicted to enable transketolase activity. Involved in pentose-phosphate shunt. Located in cytoplasm and nucleus. Orthologous to several human genes including TKT (transketolase) and TKTL2 (transketolase like 2). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:
chromosome: II
Exon count:
1
Sequence:
Chromosome: II; NC_001134.8 (474392..476437, complement)

Chromosome II - NC_001134.8Genomic Context describing neighboring genes Neighboring gene DNA repair protein RAD16 Neighboring gene L-aminoadipate-semialdehyde dehydrogenase Neighboring gene translation elongation factor EF-1 alpha Neighboring gene Mud1p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Abundances of transcripts, proteins, and metabolites in the cell cycle of budding yeast reveal coordinate control of lipid metabolism. Blank HM, et al. Mol Biol Cell, 2020 May 1. PMID 32129706, Free PMC Article
  2. Analysis of a transketolase gene from Kluyveromyces lactis reveals that the yeast enzymes are more related to transketolases of prokaryotic origins than to those of higher eukaryotes. Jacoby JJ, et al. Curr Genet, 1997 Jan. PMID 9000376
  3. Cooperative binding of substrates to transketolase from Saccharomyces cerevisiae. Sevostyanova IA, et al. Biochemistry (Mosc), 2009 Jul. PMID 19747100
  4. Theoretical model of interactions between ligand-binding sites in a dimeric protein and its application for the analysis of thiamine diphosphate binding to yeast transketolase. Ospanov R, et al. Biophys Chem, 2006 Nov 20. PMID 16837121
  5. Two methods for determination of transketolase activity. Sevostyanova IA, et al. Biochemistry (Mosc), 2006 May. PMID 16732737

See all (41) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. characterization of NQM1 and TKL2, together with TAL1 and TKL1, regarding their roles in xylose utilization and fermentation
    Title: Characterization of non-oxidative transaldolase and transketolase enzymes in the pentose phosphate pathway with regard to xylose utilization by recombinant Saccharomyces cerevisiae.
  2. In the presence of Ca2+, the active sites of TK1 and TK2 exhibit negative cooperativity in binding both xylulose-5-phosphate and ribose-5-phosphate and positive cooperativity in the catalytic transformation of the substrates.
    Title: Cooperative binding of substrates to transketolase from Saccharomyces cerevisiae.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables transketolase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables transketolase activity IEA
Inferred from Electronic Annotation
more info
  
enables transketolase activity ISS
Inferred from Sequence or Structural Similarity
more info
 PubMed 
Process Evidence Code Pubs
involved_in pentose-phosphate shunt IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in pentose-phosphate shunt IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in purine nucleotide metabolic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm HDA PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus HDA PubMed 

General protein information

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Preferred Names
transketolase TKL2
NP_009675.3
  • Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL2 has a paralog, TKL1, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001134.8 Reference assembly

    Range
    474392..476437 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001178465.3 → NP_009675.3  TPA: transketolase TKL2 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_009675.3

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VQB5, P33315
    UniProtKB/TrEMBL
    A0A6A5Q1W7, A6ZL62, B3LN38, B5VE78, C7GUL3, D3UEK9, G2W9C2, N1PAT1
    Conserved Domains (1) summary
    TIGR00232
    Location:9 → 665
    tktlase_bact; transketolase, bacterial and yeast
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P33315.1

Gene LinkOut

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