TOR1 phosphatidylinositol kinase-related protein kinase TOR1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: TOR1
Gene description: phosphatidylinositol kinase-related protein kinase TOR1
Primary source: FungiDB:YJR066W
Locus tag: YJR066W
See related: SGD:S000003827; AllianceGenome:SGD:S000003827
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: DRR1
Summary: Enables protein serine/threonine kinase activity. Involved in several processes, including cellular macromolecule biosynthetic process; mitochondria-nucleus signaling pathway; and negative regulation of protein kinase activity by regulation of protein phosphorylation. Located in bounding membrane of organelle; nucleus; and plasma membrane. Is extrinsic component of cytoplasmic side of plasma membrane. Part of TORC1 complex. Human ortholog(s) of this gene implicated in autosomal dominant polycystic kidney disease; kidney angiomyolipoma; lung disease (multiple); prostate cancer; and type 2 diabetes mellitus. Orthologous to human MTOR (mechanistic target of rapamycin kinase). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (559416..566828)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

N- and C-terminal Gln3-Tor1 interaction sites: one acting negatively and the other positively to regulate nuclear Gln3 localization.
Title: N- and C-terminal Gln3-Tor1 interaction sites: one acting negatively and the other positively to regulate nuclear Gln3 localization.
Sorting nexin Mdm1/SNX14 regulates nucleolar dynamics at the NVJ after TORC1 inactivation.
Title: Sorting nexin Mdm1/SNX14 regulates nucleolar dynamics at the NVJ after TORC1 inactivation.
Study found that TORC1 signaling is attenuated during ER stress and constitutive activation of TORC1 increases sensitivity to ER stressors independently of the unfolded protein response. TORC1 hyperactivation results in cell wall remodeling. Conversely, hyperactive TORC1 sensitizes cells to cell wall stressors, including the antifungal caspofungin.
Title: Hyperactive TORC1 sensitizes yeast cells to endoplasmic reticulum stress by compromising cell wall integrity.
Major Assembly Specificity domain from Tor1 is sufficient to confer stable association with TORC1-specific components.
Title: Redesigning TOR Kinase to Explore the Structural Basis for TORC1 and TORC2 Assembly.
Results showed that the 33-residue long FATC domain of yeast TOR1 or shorter fragments of it encompassing only the C-terminal 11-17 residue may be generally employed as C-terminal membrane anchoring unit for other proteins.
Title: Target of rapamycin FATC domain as a general membrane anchor: The FKBP-12 like domain of FKBP38 as a case study.
Data suggest that Pib2 functioning as a putative glutamine sensor in the regulation of TORC1.
Title: Gtr/Ego-independent TORC1 activation is achieved through a glutamine-sensitive interaction with Pib2 on the vacuolar membrane.
These results show that rapid activation of the TOR pathway by amino acids is not involved in rapid activation of the PKA pathway and that effects of Gcn2 inactivation as well as leu2 auxotrophy all act independently of the TOR pathway.
Title: The nutrient transceptor/PKA pathway functions independently of TOR and responds to leucine and Gcn2 in a TOR-independent manner.
We also show that Tor1p vacuolar localization and TORC1 anabolic functions, including growth promotion and phosphorylation of its direct substrate Sch9, are compromised in s-hhy mutants. Thus, an intact trans-Golgi and late endosome interface is a requisite for efficient Tor1 vacuolar localization and TORC1 function.
Title: Hygromycin B hypersensitive (hhy) mutants implicate an intact trans-Golgi and late endosome interface in efficient Tor1 vacuolar localization and TORC1 function.
Autophagy maintenance at homeostatic levels promoted by CR or TOR1 abrogation in syn-expressing cells was achieved by decreasing Sir2 levels and activity. Furthermore, the opposite function of Tor1 and Sir2 in autophagy is probably associated with the maintenance of autophagy activity at homeostatic levels
Title: Caloric restriction alleviates alpha-synuclein toxicity in aged yeast cells by controlling the opposite roles of Tor1 and Sir2 on autophagy.
DNA/RNA helicase Sen1 mutant causes modulation of protein-serine-threonine kinase TOR pathway and autophagy.
Title: Sen1, the homolog of human Senataxin, is critical for cell survival through regulation of redox homeostasis, mitochondrial function, and the TOR pathway in Saccharomyces cerevisiae.

Submit: New GeneRIF Correction See all GeneRIFs (71)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables phosphotransferase activity, alcohol group as acceptor	IEA Inferred from Electronic Annotation more info
enables protein kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein-containing complex binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in DNA damage response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in TOR signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in TORC1 signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to heat	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to nitrogen starvation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to oxidative stress	IGI Inferred from Genetic Interaction more info	PubMed
involved_in fungal-type cell wall organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondria-nucleus signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of autophagy	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of macroautophagy	IBA Inferred from Biological aspect of Ancestor more info
involved_in nucleolar large rRNA transcription by RNA polymerase I	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in peptidyl-serine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of snRNA pseudouridine synthesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of sphingolipid biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to endoplasmic reticulum stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ribosome biogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in translational initiation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Golgi membrane	IDA Inferred from Direct Assay more info	PubMed
part_of TORC1 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of TORC1 complex	IPI Inferred from Physical Interaction more info	PubMed
part_of TORC2 complex	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in endosome membrane	IDA Inferred from Direct Assay more info	PubMed
located_in fungal-type vacuole membrane	HDA more info	PubMed
located_in fungal-type vacuole membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in vacuolar membrane	IEA Inferred from Electronic Annotation more info
located_in vacuole	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: phosphatidylinositol kinase-related protein kinase TOR1

NP_012600.1

EC 2.7.11.1

PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; indole-3-acetic acid, an ATP-competitive TORC1 inhibitor, accumulates upon entry into stationary phase; malonyl-CoA is a direct ATP-competitive inhibitor of TORC1; TOR1 has a paralog, TOR2, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

559416..566828

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181724.1 → NP_012600.1 TPA: phosphatidylinositol kinase-related protein kinase TOR1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012600.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VWN7, P35169

UniProtKB/TrEMBL

C8ZBL4

Conserved Domains (7) summary

COG5032
Location:279 → 2470

TEL1; Phosphatidylinositol kinase or protein kinase, PI-3 family [Signal transduction mechanisms]

cd05169
Location:2090 → 2366

PIKKc_TOR; Catalytic domain of Target of Rapamycin

pfam02260
Location:2439 → 2470

FATC; FATC domain

pfam08771
Location:1952 → 2049

Rapamycin_bind; Rapamycin binding domain

pfam11865
Location:827 → 986

DUF3385; Domain of unknown function (DUF3385)

pfam13513
Location:771 → 820

HEAT_EZ; HEAT-like repeat

sd00044
Location:588 → 620

HEAT; HEAT repeat [structural motif]