MST1 threonine--tRNA ligase MST1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: MST1
Gene description: threonine--tRNA ligase MST1
Primary source: FungiDB:YKL194C
Locus tag: YKL194C
See related: SGD:S000001677; AllianceGenome:SGD:S000001677
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables threonine-tRNA ligase activity. Involved in mitochondrial threonyl-tRNA aminoacylation. Located in mitochondrion. Orthologous to human MRPL39 (mitochondrial ribosomal protein L39). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: XI

Exon count:: 1

Sequence:: Chromosome: XI; NC_001143.9 (77258..78646, complement)

Chromosome XI - NC_001143.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001143.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data suggest that structural features of the anticodon-binding domain in threonine--tRNA ligase MST1 permit binding of the enlarged anticodon loop of tRNA1(Thr).
Title: The crystal structure of yeast mitochondrial ThrRS in complex with the canonical threonine tRNA.
the plasticity of the aminoacylation site in MST1 allows binding of Ser-AMP and the appropriate positioning of the hydrolytic water molecule.
Title: The mechanism of pre-transfer editing in yeast mitochondrial threonyl-tRNA synthetase.
MST1 recognizes and aminoacylates two natural tRNAs that contain anticodon loops of different size and sequence
Title: Yeast mitochondrial threonyl-tRNA synthetase recognizes tRNA isoacceptors by distinct mechanisms and promotes CUN codon reassignment.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables aminoacyl-tRNA ligase activity	IEA Inferred from Electronic Annotation more info
enables catalytic activity, acting on a tRNA	IEA Inferred from Electronic Annotation more info
enables threonine-tRNA ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables threonine-tRNA ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables threonine-tRNA ligase activity	IEA Inferred from Electronic Annotation more info
enables threonine-tRNA ligase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in mitochondrial threonyl-tRNA aminoacylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitochondrial threonyl-tRNA aminoacylation	IDA Inferred from Direct Assay more info	PubMed
involved_in tRNA aminoacylation for protein translation	IEA Inferred from Electronic Annotation more info
involved_in threonyl-tRNA aminoacylation	IEA Inferred from Electronic Annotation more info
involved_in translation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in mitochondrial matrix	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: threonine--tRNA ligase MST1

NP_012727.1

EC 6.1.1.3

Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(UAG)Q2 in vitro; lacks a typical editing domain, but has pre-transfer editing activity stimulated by the unusual tRNA-Thr

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001143.9 Reference assembly

Range

77258..78646 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179760.1 → NP_012727.1 TPA: threonine--tRNA ligase MST1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012727.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VX06, P07236

UniProtKB/TrEMBL

A0A8H4FBU7

Conserved Domains (2) summary

cd00771
Location:44 → 347

ThrRS_core; Threonyl-tRNA synthetase (ThrRS) class II core catalytic domain. ThrRS is a homodimer. It is responsible for the attachment of threonine to the 3' OH group of ribose of the appropriate tRNA. This domain is primarily responsible for ATP-dependent ...

cd00860
Location:347 → 459

ThrRS_anticodon; ThrRS Threonyl-anticodon binding domain. ThrRS belongs to class II aminoacyl-tRNA synthetases (aaRS). This alignment contains the anticodon binding domain, which is responsible for specificity in tRNA-binding, so that the activated amino acid is ...