GAP1 amino acid permease GAP1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: GAP1
Gene description: amino acid permease GAP1
Primary source: SGD:S000001747
Locus tag: YKR039W
See related: AllianceGenome:SGD:S000001747
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables L-proline transmembrane transporter activity; beta-alanine transmembrane transporter activity; and polyamine transmembrane transporter activity. Involved in amino acid transport and polyamine transport. Located in cytoplasmic vesicle; fungal-type vacuole lumen; and plasma membrane. Is integral component of plasma membrane. Orthologous to several human genes including SLC7A1 (solute carrier family 7 member 1); SLC7A2 (solute carrier family 7 member 2); and SLC7A3 (solute carrier family 7 member 3). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: XI

Exon count:: 1

Sequence:: Chromosome: XI; NC_001143.9 (515063..516871)

Chromosome XI - NC_001143.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001143.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Nutrient transceptors physically interact with the yeast S6/protein kinase B homolog, Sch9, a TOR kinase target.
Title: Nutrient transceptors physically interact with the yeast S6/protein kinase B homolog, Sch9, a TOR kinase target.
Using in silico analyses and mutational studies we found that the C-terminal sequences of Gap1, Bap2, Hip1, Tat1, Tat2, Mmp1, Sam3, Agp1, and Gnp1 are about 50 residues long, associate with the Plasma Membrane , and have features that discriminate them from the termini of organellar amino acid transporters
Title: A Plasma Membrane Association Module in Yeast Amino Acid Transporters.
The results presented here suggest some possible synergistic effects of phosphorylated Alp1, Gap1 and Can1 amino acid permeases on regulation of amino acid utilization and metabolism in Saccharomyces cerevisiae.
Title: Effects of three permeases on arginine utilization in Saccharomyces cerevisiae.
results support the concept that different substrates bind to partially overlapping binding sites in the same general substrate-binding pocket of Gap1, triggering divergent conformations, resulting in different conformation-induced downstream processes
Title: Specific analogues uncouple transport, signalling, oligo-ubiquitination and endocytosis in the yeast Gap1 amino acid transceptor.
Although the Bul proteins mediate Gap1 ubiquitylation of two possible lysines, Lys-9 and Lys-16, the Aly proteins promote ubiquitylation of the Lys-16 residue only. This stress-induced pathway of Gap1 down-regulation targets other permeases as well.
Title: Stress conditions promote yeast Gap1 permease ubiquitylation and down-regulation via the arrestin-like Bul and Aly proteins.
study show that Gap1 and Can1 permeases undergo ubiquitin-dependent endocytosis in response to their substrates and that this endocytosis is due to transport catalysis rather than intracellular substrate accumulation.
Title: Substrate-induced ubiquitylation and endocytosis of yeast amino acid permeases.
a possible mechanism involved in the regulation of Rsp5 activity for Gap1 down-regulation via the phosphorylation of a conserved Thr357 in the Nedd4 family is proposed
Title: Phosphorylation of a conserved Thr357 in yeast Nedd4-like ubiquitin ligase Rsp5 is involved in down-regulation of the general amino acid permease Gap1.
Internal amino acids promote Gap1 permease ubiquitylation via TORC1/Npr1/14-3-3-dependent control of the Bul arrestin-like adaptors.
Title: Internal amino acids promote Gap1 permease ubiquitylation via TORC1/Npr1/14-3-3-dependent control of the Bul arrestin-like adaptors.
Transport activity-dependent intracellular sorting of the yeast general amino acid permease
Title: Transport activity-dependent intracellular sorting of the yeast general amino acid permease.
identified a self-propagating extrachromosomal circular DNA molecule that results from intrachromosomal recombination between long terminal repeats flanking GAP1
Title: Adaptation to diverse nitrogen-limited environments by deletion or extrachromosomal element formation of the GAP1 locus.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables L-proline transmembrane transporter activity	IGI Inferred from Genetic Interaction more info	PubMed
enables amino acid transmembrane transporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables amino acid transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables beta-alanine transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables polyamine transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables transmembrane transporter activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in amino acid transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in amino acid transport	IEA Inferred from Electronic Annotation more info
involved_in amino acid transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in beta-alanine transport	IEA Inferred from Electronic Annotation more info
involved_in nitrogen compound transport	IEA Inferred from Electronic Annotation more info
involved_in organic substance transport	IEA Inferred from Electronic Annotation more info
involved_in phenylalanine transport	IEA Inferred from Electronic Annotation more info
involved_in polyamine transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in polyamine transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proline transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in transmembrane transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in COPII-coated ER to Golgi transport vesicle	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	HDA more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in endosome	IDA Inferred from Direct Assay more info	PubMed
located_in fungal-type vacuole lumen	IDA Inferred from Direct Assay more info	PubMed
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in multivesicular body	IMP Inferred from Mutant Phenotype more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: amino acid permease GAP1

NP_012965.3

General amino acid permease; involved in uptake of all L-amino acids, some D-amino acids, related compounds, toxic analogs and polyamines; regulated by nitrogen source; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway; essential for invasive growth

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001143.9 Reference assembly

Range

515063..516871

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179829.3 → NP_012965.3 TPA: amino acid permease GAP1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012965.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VXA2, P19145

UniProtKB/TrEMBL

A0A1B1SPG0, A0A8H4C0L6, A7A001, B3LRC0, N1NZH2

Conserved Domains (1) summary

COG0833
Location:43 → 601

LysP; Amino acid permease [Amino acid transport and metabolism]