FOL1 trifunctional dihydropteroate synthetase/dihydrohydroxymethylpterin pyrophosphokinase/dihydroneopterin aldolase FOL1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: FOL1
Gene description: trifunctional dihydropteroate synthetase/dihydrohydroxymethylpterin pyrophosphokinase/dihydroneopterin aldolase FOL1
Primary source: SGD:S000005200
Locus tag: YNL256W
See related: AllianceGenome:SGD:S000005200
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity; dihydroneopterin aldolase activity; and dihydropteroate synthase activity. Involved in tetrahydrofolate biosynthetic process. Located in mitochondrial envelope. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (164623..167097)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

FOL1 is an essential gene and the nongrowth phenotype of the fol1 deletion leads to a recessive auxotrophy for folinic acid (5'-formyltetrahydrofolate).
Title: Characterization of the Saccharomyces cerevisiae Fol1 protein: starvation for C1 carrier induces pseudohyphal growth.
Results describe the crystal structure of 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase/dihydropteroate synthase of Saccharomyces cerevisiae at 2.3 angstrom resolution.
Title: The three-dimensional structure of the bifunctional 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase/dihydropteroate synthase of Saccharomyces cerevisiae.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity	IDA Inferred from Direct Assay more info	PubMed
enables 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity	IEA Inferred from Electronic Annotation more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables dihydroneopterin aldolase activity	IDA Inferred from Direct Assay more info	PubMed
enables dihydroneopterin aldolase activity	IEA Inferred from Electronic Annotation more info
enables dihydropteroate synthase activity	IBA Inferred from Biological aspect of Ancestor more info
enables dihydropteroate synthase activity	IDA Inferred from Direct Assay more info	PubMed
enables dihydropteroate synthase activity	IEA Inferred from Electronic Annotation more info
enables dihydropteroate synthase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables lyase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in folic acid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in folic acid-containing compound biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in tetrahydrofolate biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in tetrahydrofolate biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in tetrahydrofolate biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in mitochondrial envelope	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrial envelope	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: trifunctional dihydropteroate synthetase/dihydrohydroxymethylpterin pyrophosphokinase/dihydroneopterin aldolase FOL1

NP_014143.2

EC 2.5.1.15

EC 2.7.6.3

EC 4.1.2.25

Multifunctional enzyme of the folic acid biosynthesis pathway; has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

164623..167097

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183094.1 → NP_014143.2 TPA: trifunctional dihydropteroate synthetase/dihydrohydroxymethylpterin pyrophosphokinase/dihydroneopterin aldolase FOL1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014143.2

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W0T7, P53848, Q2VQX0

UniProtKB/TrEMBL

A0A8H4BVH4, B3LP99, C7GPA5

Conserved Domains (4) summary

COG0801
Location:297 → 460

FolK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase [Coenzyme transport and metabolism]

cd00739
Location:504 → 817

DHPS; DHPS subgroup of Pterin binding enzymes. DHPS (dihydropteroate synthase), a functional homodimer, catalyzes the condensation of p-aminobenzoic acid (pABA) in the de novo biosynthesis of folate, which is an essential cofactor in both nucleic acid and ...

TIGR00526
Location:21 → 138

folB_dom; FolB domain

cl00263
Location:146 → 259

TFold; Tunnelling fold (T-fold). The five known T-folds are found in five different enzymes with different functions: dihydroneopterin-triphosphate epimerase (DHNTPE), dihydroneopterin aldolase (DHNA) , GTP cyclohydrolase I (GTPCH-1), 6-pyrovoyl ...