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PUS1 pseudouridine synthase PUS1 [ Saccharomyces cerevisiae S288C ]

Gene ID: 855889, updated on 11-Apr-2024

Summary

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Gene symbol
PUS1
Gene description
pseudouridine synthase PUS1
Primary source
SGD:S000006133
Locus tag
YPL212C
See related
AllianceGenome:SGD:S000006133
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables pseudouridine synthase activity. Involved in mRNA pseudouridine synthesis; snRNA pseudouridine synthesis; and tRNA pseudouridine synthesis. Located in nucleus. Human ortholog(s) of this gene implicated in myopathy, lactic acidosis, and sideroblastic anemia 1. Orthologous to human PUS1 (pseudouridine synthase 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:
chromosome: XVI
Exon count:
1
Sequence:
Chromosome: XVI; NC_001148.4 (151515..153149, complement)

Chromosome XVI - NC_001148.4Genomic Context describing neighboring genes Neighboring gene bifunctional hydroxyethylthiazole kinase/thiamine-phosphate diphosphorylase Neighboring gene U2 snRNP complex subunit LEA1 Neighboring gene ribosome biosynthesis protein NIP7 Neighboring gene signal recognition particle subunit SRP72

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. A previously unidentified activity of yeast and mouse RNA:pseudouridine synthases 1 (Pus1p) on tRNAs. Behm-Ansmant I, et al. RNA, 2006 Aug. PMID 16804160, Free PMC Article
  2. RNA:pseudouridine synthetase Pus1 from Saccharomyces cerevisiae: oligomerization property and stoichiometry of the complex with yeast tRNA(Phe). Arluison V, et al. Biochimie, 1999 Jul. PMID 10492022
  3. Pseudouridine synthetase Pus1 of Saccharomyces cerevisiae: kinetic characterisation, tRNA structural requirement and real-time analysis of its complex with tRNA. Arluison V, et al. J Mol Biol, 1999 Jun 11. PMID 10356324
  4. Pseudouridine mapping in the Saccharomyces cerevisiae spliceosomal U small nuclear RNAs (snRNAs) reveals that pseudouridine synthase pus1p exhibits a dual substrate specificity for U2 snRNA and tRNA. Massenet S, et al. Mol Cell Biol, 1999 Mar. PMID 10022901, Free PMC Article
  5. The yeast tRNA:pseudouridine synthase Pus1p displays a multisite substrate specificity. Motorin Y, et al. RNA, 1998 Jul. PMID 9671058, Free PMC Article

See all (64) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Impact of Pus1 Pseudouridine Synthase on Specific Decoding Events in Saccharomyces cerevisiae.
    Title: Impact of Pus1 Pseudouridine Synthase on Specific Decoding Events in Saccharomyces cerevisiae.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables isomerase activity IEA
Inferred from Electronic Annotation
more info
  
enables mRNA binding HDA PubMed 
enables pseudouridine synthase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables pseudouridine synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables pseudouridine synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables snRNA pseudouridine synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables tRNA pseudouridine synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
Process Evidence Code Pubs
involved_in mRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in mRNA pseudouridine synthesis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in mRNA pseudouridine synthesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in snRNA pseudouridine synthesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in tRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in tRNA pseudouridine synthesis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in tRNA pseudouridine synthesis IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in tRNA pseudouridine synthesis IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in intracellular membrane-bounded organelle IEA
Inferred from Electronic Annotation
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
pseudouridine synthase PUS1
NP_015112.1
  • tRNA:pseudouridine synthase; introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; also acts on U2 snRNA; also pseudouridylates some mRNAs, and pseudouridylation level varies with growth phase; nuclear protein that appears to be involved in tRNA export; PUS1 has a paralog, PUS2, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001148.4 Reference assembly

    Range
    151515..153149 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001184026.1 → NP_015112.1  TPA: pseudouridine synthase PUS1 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_015112.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6W3F8, Q12211
    UniProtKB/TrEMBL
    A0A8H4BUF4, A6ZW43, B3LKL8, C7GN39, C8ZIG7, N1NWM6
    Conserved Domains (1) summary
    cd02568
    Location:79 → 407
    PseudoU_synth_PUS1_PUS2; Pseudouridine synthase, PUS1/ PUS2 like
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q12211.1

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