U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

nhaA Na(+):H(+) antiporter NhaA [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 944758, updated on 11-Apr-2024

Summary

Go to the top of the page Help
Gene symbol
nhaA
Gene description
Na(+):H(+) antiporter NhaA
Primary source
ASAP:ABE-0000068
Locus tag
b0019
See related
ECOCYC:EG10652
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ant; antA; ECK0020
Summary
nhaA P2 promoter is RpoS-regulated, but H-NS, Na(+)- and NhaR-independent. [More information is available at EcoGene: EG10652]. NhaA is a sodium ion/proton antiporter that uses the proton electrochemical gradient to expel sodium ions from the cytoplasm and functions primarily in the adaptation to high salinity at alkaline pH. [More information is available at EcoCyc: EG10652].
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
Sequence:
NC_000913.3 (17489..18655)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene protein HokC Neighboring gene regulatory protein MokC Neighboring gene ncRNA Neighboring gene DNA-binding transcriptional activator NhaR Neighboring gene IS1 family protein InsB Neighboring gene IS1 family protein InsA

Genomic regions, transcripts, and products

Go to the top of the page Help
Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Towards Molecular Understanding of the pH Dependence Characterizing NhaA of Which Structural Fold is Shared by Other Transporters. Mondal R, et al. J Mol Biol, 2021 Sep 17. PMID 34273399
  2. An angular motion of a conserved four-helix bundle facilitates alternating access transport in the TtNapA and EcNhaA transporters. Masrati G, et al. Proc Natl Acad Sci U S A, 2020 Dec 15. PMID 33257549, Free PMC Article
  3. Alternative proton-binding site and long-distance coupling in Escherichia coli sodium-proton antiporter NhaA. Henderson JA, et al. Proc Natl Acad Sci U S A, 2020 Oct 13. PMID 32973095, Free PMC Article
  4. Replacement of Lys-300 with a glutamine in the NhaA Na(+)/H(+) antiporter of Escherichia coli yields a functional electrogenic transporter. Patiño-Ruiz M, et al. J Biol Chem, 2019 Jan 4. PMID 30409911, Free PMC Article
  5. Lysine 300 is essential for stability but not for electrogenic transport of the Escherichia coli NhaA Na(+)/H(+) antiporter. Călinescu O, et al. J Biol Chem, 2017 May 12. PMID 28330875, Free PMC Article

See all (118) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Towards Molecular Understanding of the pH Dependence Characterizing NhaA of Which Structural Fold is Shared by Other Transporters.
    Title: Towards Molecular Understanding of the pH Dependence Characterizing NhaA of Which Structural Fold is Shared by Other Transporters.
  2. An angular motion of a conserved four-helix bundle facilitates alternating access transport in the TtNapA and EcNhaA transporters.
    Title: An angular motion of a conserved four-helix bundle facilitates alternating access transport in the TtNapA and EcNhaA transporters.
  3. Alternative proton-binding site and long-distance coupling in Escherichia coli sodium-proton antiporter NhaA.
    Title: Alternative proton-binding site and long-distance coupling in Escherichia coli sodium-proton antiporter NhaA.
  4. findings indicate that alternative mechanisms sustain EcNhaA activity in the absence of canonical ion-binding residues and that the conserved lysines confer structural stability.
    Title: Replacement of Lys-300 with a glutamine in the NhaA Na(+)/H(+) antiporter of Escherichia coli yields a functional electrogenic transporter.
  5. analysis of NhaA pH-dependent activation by molecular dynamics simulation of atomic details of proton-coupled transport across membrane
    Title: Mechanism of pH-dependent activation of the sodium-proton antiporter NhaA.
  6. Lysine 300 has an essential role in stability but not electrogenic transport of Escherichia coli NhaA
    Title: Lysine 300 is essential for stability but not for electrogenic transport of the Escherichia coli NhaA Na(+)/H(+) antiporter.
  7. Data show that the Li(+) binding, H(+) release and antiporter activity were all affected to the same extent by Na(+), Li(+)/H(+) antiporter (NhaA) mutations in the Li(+) binding site (D163E, D163N, D164N, D164E).
    Title: The Ec-NhaA antiporter switches from antagonistic to synergistic antiport upon a single point mutation.
  8. Data show that the two aspartic acid residues of Na+/H+ antiporter NhaA, D163 and D164, act as Na+ trap and energetic barrier for the transport of Na+.
    Title: Simulating the function of sodium/proton antiporters.
  9. NhaA antiporter functions using 10 helices, and an additional 2 contribute to assembly/stability.
    Title: NhaA antiporter functions using 10 helices, and an additional 2 contribute to assembly/stability.
  10. As it has been previously suggested that Asp163 is one of the two residues through which proton transport occurs, these results have clear implications to the current mechanistic models of sodium-proton antiport in NhaA.
    Title: Crystal structure of the sodium-proton antiporter NhaA dimer and new mechanistic insights.
Submit: New GeneRIF Correction See all GeneRIFs (26)

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables antiporter activity IEA
Inferred from Electronic Annotation
more info
  
enables cardiolipin binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables sodium ion transmembrane transporter activity IEA
Inferred from Electronic Annotation
more info
  
enables sodium:proton antiporter activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables sodium:proton antiporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in monoatomic ion transport IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within regulation of intracellular pH TAS
Traceable Author Statement
more info
 PubMed 
involved_in regulation of pH IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within response to alkaline pH IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within response to alkaline pH IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within response to salt stress IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in sodium ion transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in sodium ion transport IEA
Inferred from Electronic Annotation
more info
  
involved_in transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane ISM
Inferred from Sequence Model
more info
 PubMed 

General protein information

Go to the top of the page Help
Preferred Names
Na(+):H(+) antiporter NhaA

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    17489..18655
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414560.1 Na(+):H(+) antiporter NhaA [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_414560.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A6N8NMK2, A0A8S7CVL3, A0A8S7I7R4
    Conserved Domains (1) summary
    PRK09561
    Location:1 → 388
    nhaA; sodium/proton antiporter NhaA
Nucleotide Protein
Heading Accession and Version

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.