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carB carbamoyl-phosphate synthetase large subunit [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 944775, updated on 11-Apr-2024

Summary

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Gene symbol
carB
Gene description
carbamoyl-phosphate synthetase large subunit
Primary source
ASAP:ABE-0000121
Locus tag
b0033
See related
ECOCYC:EG10135
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0034; pyrA
Summary
The large subunit is the synthetase component of the enzyme. [More information is available at EcoCyc: EG10135].
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Genomic context

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Sequence:
NC_000913.3 (30817..34038)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene 4-hydroxy-tetrahydrodipicolinate reductase Neighboring gene carbamoyl-phosphate synthetase small subunit Neighboring gene DNA-binding transcriptional activator CaiF Neighboring gene putative transferase CaiE

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Site-directed mutagenesis of the regulatory domain of Escherichia coli carbamoyl phosphate synthetase identifies crucial residues for allosteric regulation and for transduction of the regulatory signals. Fresquet V, et al. J Mol Biol, 2000 Jun 16. PMID 10843852
  2. Regulatory changes in the control of carbamoyl phosphate synthetase induced by truncation and mutagenesis of the allosteric binding domain. Czerwinski RM, et al. Biochemistry, 1995 Oct 24. PMID 7577987
  3. Transcription start site sequence and spacing between the -10 region and the start site affect reiterative transcription-mediated regulation of gene expression in Escherichia coli. Han X, et al. J Bacteriol, 2014 Aug 15. PMID 24891446, Free PMC Article
  4. A combined theoretical and experimental study of the ammonia tunnel in carbamoyl phosphate synthetase. Fan Y, et al. J Am Chem Soc, 2009 Jul 29. PMID 19569682, Free PMC Article
  5. Mutation analysis of carbamoyl phosphate synthetase: does the structurally conserved glutamine amidotransferase triad act as a functional dyad? Hart EJ, et al. Protein Sci, 2008 Jul. PMID 18458150, Free PMC Article

See all (62) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. These results indicate that transcription start site sequence and spacing between the -10 region and the start site are important determinants in establishing the extent of reiterative transcription and levels of productive transcription.
    Title: Transcription start site sequence and spacing between the -10 region and the start site affect reiterative transcription-mediated regulation of gene expression in Escherichia coli.
  2. N-terminus verified by Edman degradation on mature peptide
    Title: The carB gene of Escherichia coli: a duplicated gene coding for the large subunit of carbamoyl-phosphate synthetase.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables ATP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables amino acid binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables carbamoyl-phosphate synthase (ammonia) activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables carbamoyl-phosphate synthase (ammonia) activity IEA
Inferred from Electronic Annotation
more info
  
contributes_to carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity IBA
Inferred from Biological aspect of Ancestor
more info
  
contributes_to carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity IEA
Inferred from Electronic Annotation
more info
  
contributes_to carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity IGI
Inferred from Genetic Interaction
more info
 PubMed 
contributes_to carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables ligase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in 'de novo' UMP biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in amino acid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within amino acid biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in arginine biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in arginine biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in arginine biosynthetic process NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in glutamine metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within pyrimidine nucleobase biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in pyrimidine nucleobase biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in pyrimidine nucleotide biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of carbamoyl-phosphate synthase complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of carbamoyl-phosphate synthase complex IPI
Inferred from Physical Interaction
more info
 PubMed 
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
carbamoyl-phosphate synthetase large subunit
NP_414574.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    30817..34038
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414574.1 carbamoyl-phosphate synthetase large subunit [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_414574.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A5B9AAI1
    Conserved Domains (1) summary
    PRK05294
    Location:1 → 1069
    carB; carbamoyl-phosphate synthase large subunit
Nucleotide Protein
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