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ispH 4-hydroxy-3-methylbut-2-enyl diphosphate reductase [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 944777, updated on 2-May-2024

Summary

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Gene symbol
ispH
Gene description
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Primary source
ASAP:ABE-0000103
Locus tag
b0029
See related
ECOCYC:EG11081
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0030; lytB; yaaE
Summary
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (IspH) catalyzes what is effectively the final step in the methylerythritol phosphate pathway, generating the two pathway products isopentyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP). [More information is available at EcoCyc: EG11081].
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Genomic context

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Sequence:
NC_000913.3 (26277..27227)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene isoleucine--tRNA ligase Neighboring gene lipoprotein signal peptidase Neighboring gene peptidyl-prolyl cis-trans isomerase FkpB Neighboring gene ribonucleoside hydrolase RihC Neighboring gene 4-hydroxy-tetrahydrodipicolinate reductase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The Reductive Dehydroxylation Catalyzed by IspH, a Source of Inspiration for the Development of Novel Anti-Infectives. Jobelius H, et al. Molecules, 2022 Jan 21. PMID 35163971, Free PMC Article
  2. Discovery of acetylene hydratase activity of the iron-sulphur protein IspH. Span I, et al. Nat Commun, 2012. PMID 22948824, Free PMC Article
  3. Isoprenoid biosynthesis via the MEP pathway: in vivo Mössbauer spectroscopy identifies a [4Fe-4S]2+ center with unusual coordination sphere in the LytB protein. Seemann M, et al. J Am Chem Soc, 2009 Sep 23. PMID 19708647
  4. Revisiting the IspH catalytic system in the deoxyxylulose phosphate pathway: achieving high activity. Xiao Y, et al. J Am Chem Soc, 2009 Jul 29. PMID 19583210
  5. Structure of active IspH enzyme from Escherichia coli provides mechanistic insights into substrate reduction. Gräwert T, et al. Angew Chem Int Ed Engl, 2009. PMID 19569147

See all (44) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The Reductive Dehydroxylation Catalyzed by IspH, a Source of Inspiration for the Development of Novel Anti-Infectives.
    Title: The Reductive Dehydroxylation Catalyzed by IspH, a Source of Inspiration for the Development of Novel Anti-Infectives.
  2. work for the first time identified HMBPP to be a cytotoxic intermediate in MEP pathway and demonstrated that balanced activation of IspG and IspH could eliminate accumulation of HMBPP and MECPP and improve isoprenoids production.
    Title: Balanced activation of IspG and IspH to eliminate MEP intermediate accumulation and improve isoprenoids production in Escherichia coli.
  3. IspH can hydrate acetylenes to aldehydes and ketones via anti-Markovnikov/Markovnikov addition.
    Title: Discovery of acetylene hydratase activity of the iron-sulphur protein IspH.
  4. In LytB an unusual [4Fe-4S](2+) cluster is attached to the protein by three conserved cysteines and contains a hexacoordinated iron linked to three sulfurs of the cluster and three additional oxygen or nitrogen ligands.
    Title: Isoprenoid biosynthesis via the MEP pathway: in vivo Mössbauer spectroscopy identifies a [4Fe-4S]2+ center with unusual coordination sphere in the LytB protein.
  5. a structure-based rationale of the substrate binding mode of ispH, which in combination with mutational studies and theoretical calculation supports a reaction mechanism representing a biological counterpart of the Birch reduction of allylic alcohols.
    Title: Structure of active IspH enzyme from Escherichia coli provides mechanistic insights into substrate reduction.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables 3 iron, 4 sulfur cluster binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables 4 iron, 4 sulfur cluster binding IEA
Inferred from Electronic Annotation
more info
  
enables 4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydroxymethylbutenyl pyrophosphate reductase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables oxidoreductase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in dimethylallyl diphosphate biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in isoprenoid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in terpenoid biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
NP_414570.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    26277..27227
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414570.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_414570.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A4C7B5J3, A0A5B9ABS9
    Conserved Domains (1) summary
    PRK01045
    Location:1 → 301
    ispH; 4-hydroxy-3-methylbut-2-enyl diphosphate reductase; Reviewed
Nucleotide Protein
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