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ftsI peptidoglycan DD-transpeptidase FtsI [ Escherichia coli str. K-12 substr. MG1655 ]

Gene ID: 944799, updated on 11-Apr-2024

Summary

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Gene symbol
ftsI
Gene description
peptidoglycan DD-transpeptidase FtsI
Primary source
ASAP:ABE-0000309
Locus tag
b0084
See related
ECOCYC:EG10341
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage
Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as
ECK0085; pbpB; sep
Summary
FtsI is recruited by its cognate SEDS protein FtsW. [More information is available at EcoGene: EG10341]. FtsI (penicillin-binding protein 3, PBP3) is an essential cell division protein which is present at low abundance of about 100 molecules per cell . [More information is available at EcoCyc: EG10341].
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Genomic context

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Sequence:
NC_000913.3 (91413..93179)

NC_000913.3Genomic Context describing neighboring genes Neighboring gene DNA-binding transcriptional repressor MraZ Neighboring gene 16S rRNA m(4)C1402 methyltransferase Neighboring gene cell division protein FtsL Neighboring gene ncRNA Neighboring gene UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase Neighboring gene D-alanyl-D-alanine-adding enzyme Neighboring gene phospho-N-acetylmuramoyl-pentapeptide-transferase Neighboring gene UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase Neighboring gene peptidoglycan glycosyltransferase FtsW

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Stationary phase persister formation in Escherichia coli can be suppressed by piperacillin and PBP3 inhibition. Aedo SJ, et al. BMC Microbiol, 2019 Jun 24. PMID 31234796, Free PMC Article
  2. Unusual Escherichia coli PBP 3 Insertion Sequence Identified from a Collection of Carbapenem-Resistant Enterobacteriaceae Tested In Vitro with a Combination of Ceftazidime-, Ceftaroline-, or Aztreonam-Avibactam. Zhang Y, et al. Antimicrob Agents Chemother, 2017 Aug. PMID 28559260, Free PMC Article
  3. Differential functionalities of amphiphilic peptide segments of the cell-septation penicillin-binding protein 3 of Escherichia coli. Marrec-Fairley M, et al. Mol Microbiol, 2000 Sep. PMID 10972821
  4. Visualization of membrane domains in Escherichia coli. Fishov I, et al. Mol Microbiol, 1999 Jun. PMID 10383758
  5. The structure and function of Escherichia coli penicillin-binding protein 3. Nguyen-Distèche M, et al. Cell Mol Life Sci, 1998 Apr. PMID 9614966

See all (96) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Data suggest that piperacillin and more generally PBP3 inhibition during stationary phase produces a phenotypic state characterized by large abundance of essential growth materials that render cells more uniformly susceptible to the bactericidal activities of beta-lactams and fluoroquinolones.
    Title: Stationary phase persister formation in Escherichia coli can be suppressed by piperacillin and PBP3 inhibition.
  2. A beta-lactam MIC of 8 mug/ml was reported for the three combinations against one Escherichia coli isolate with an unusual TIPY insertion following Tyr344 in penicillin-binding protein 3 (PBP 3) as the result of gene duplication.
    Title: Unusual Escherichia coli PBP 3 Insertion Sequence Identified from a Collection of Carbapenem-Resistant Enterobacteriaceae Tested In Vitro with a Combination of Ceftazidime-, Ceftaroline-, or Aztreonam-Avibactam.
  3. In this work the authors show direct interactions between FtsW and PBP3 in vivo and in vitro by FRET (Forster resonance energy transfer) and co-immunoprecipitation experiments.
    Title: The integral membrane FtsW protein and peptidoglycan synthase PBP3 form a subcomplex in Escherichia coli.
  4. Data show that PBP3 interact with MtgA and help MtgA's role in peptidoglycan assembly during the cell cycle.
    Title: The monofunctional glycosyltransferase of Escherichia coli localizes to the cell division site and interacts with penicillin-binding protein 3, FtsW, and FtsN.
  5. FtsI is associated in a hierarchical set of protein complexes and its assembly can occur independently of 'upstream' proteins.
    Title: Premature targeting of cell division proteins to midcell reveals hierarchies of protein interactions involved in divisome assembly.
  6. Alanine-scanning mutagenesis of the Transmembrane helix identified several residues important for septal localization.
    Title: The transmembrane helix of the Escherichia coli division protein FtsI localizes to the septal ring.
  7. direct interaction between a class B PBP (PBP3) and a class A PBP (PBP1B) in vitro and in vivo, indicating that different murein synthases might act in concert to enlarge the murein sacculus during cell division
    Title: Interaction between two murein (peptidoglycan) synthases, PBP3 and PBP1B, in Escherichia coli.
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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Gene Ontology Provided by EcoCyc

Function Evidence Code Pubs
enables carboxypeptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables penicillin binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables penicillin binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables penicillin binding IEA
Inferred from Electronic Annotation
more info
  
enables peptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables peptidoglycan glycosyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables peptidoglycan glycosyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables peptidoglycan glycosyltransferase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables serine-type D-Ala-D-Ala carboxypeptidase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in FtsZ-dependent cytokinesis IEA
Inferred from Electronic Annotation
more info
  
involved_in FtsZ-dependent cytokinesis NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in cell cycle IEA
Inferred from Electronic Annotation
more info
  
involved_in cell division IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within cell division IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in cell division NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in cell wall organization IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in cell wall organization IEA
Inferred from Electronic Annotation
more info
  
involved_in division septum assembly IEA
Inferred from Electronic Annotation
more info
  
involved_in division septum assembly NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in peptidoglycan biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within peptidoglycan biosynthetic process RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
involved_in proteolysis IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of cell shape IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within response to xenobiotic stimulus IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cell division site IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cell division site NAS
Non-traceable Author Statement
more info
 PubMed 
part_of divisome complex NAS
Non-traceable Author Statement
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane NAS
Non-traceable Author Statement
more info
 PubMed 

General protein information

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Preferred Names
peptidoglycan DD-transpeptidase FtsI

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_000913.3 Reference assembly

    Range
    91413..93179
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NP_414626.1 peptidoglycan DD-transpeptidase FtsI [Escherichia coli str. K-12 substr. MG1655]

    See identical proteins and their annotated locations for NP_414626.1

    Status: PROVISIONAL

    UniProtKB/TrEMBL
    A0A3G1CH64
    Conserved Domains (1) summary
    PRK15105
    Location:2 → 579
    PRK15105; peptidoglycan synthase FtsI; Provisional
Nucleotide Protein
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